Abstract
The dependence of somatic hypermutation on transcription was studied in three mutant immunoglobulin heavy chain (IgH) insertion mice in which a targeted nonfunctional VHB1-8 passenger transgene was either placed under the transcriptional control of a truncated DQ52 promoter (pΔ), its own RNA polymerase II dependent IgH promoter (pII) or a RNA polymerase I dependent promoter (pI). The relative mutation-frequency of the VhBI-8 passenger transgene in memory B cells of pΔ, pi and pII mice (7%, 60% and 100%) correlated with the relative levels of transgene-specific pre-mRNA expressed in germinal center B cells isolated from the mutant mice (8%, 72% and 100%, respectively). These data indicate that the mutation load of rearranged Ig genes can be tuned by transcription. The question, whether somatic hypermutation requires transcription per se or a specific component of the RNA polymerase II complex, is under investigation.
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Jacobs, H., Puglisi, A., Rajewsky, K., Fukita, Y. (1999). Tuning Somatic Hypermutation by Transcription. In: Melchers, F., Potter, M. (eds) Mechanisms of B Cell Neoplasia 1998. Current Topics in Microbiology and Immunology, vol 246. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60162-0_19
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DOI: https://doi.org/10.1007/978-3-642-60162-0_19
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