Abstract
The wealth of sequence data generated in the last 20 years has revealed the existence of many multigene families (MacIntyre 1994; Ohta 1991). Genes within a family typically share short stretches of conserved primary sequence, usually as part of conserved functional domains. Genes within a family may also play similar or overlapping roles in diverse tissues and processes. In some cases, individual genes within a family are differentially expressed, with up-regulation coinciding with a requirement for the gene product in a specific biological process. Examples include the differential expression of homeobox containing genes during development (Lawrence and Morata 1994; Scott and Weiner 1984) and altered abundance of members of the cyclin gene family during various phases of the cell cycle (Pines 1995; Hunt 1991). Thus, for processes in which specific gene families are suspected to play a role, an assay that allows for the rapid detection and cloning of differentially expressed family members would be of great value. Targeted RNA fingerprinting (TRF) was developed to respond to such a need.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Chomczynski P, Sacchi N (1987) Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction. Anal Biochem 162:156–159
Hunt T (1991) Cyclins and their partners: from a simple idea to complicated reality. Seminars in Cell Biology 2:213–222
Lathe R (1985) Synthetic Oligonucleotide Probes Deduced from Amino Acid Sequence Data. J Mol Biol 183:1–12
Lawrence P A, Morata G (1994) Homeobox Genes: Their Function in Drosophila Segmentation and Pattern Formation. Cell 78:181–189
Liang P, Pardee A B (1992) Differential Display of Eukaryotic Messenger RNA by Means of the Polymerase Chain Reaction. Science 257:967–971
MacIntyre R J (1994) Molecular evolution: codes, clocks, genes and genomes. BioEssays 16(9):699–703
Ohta T (1991) Multigene families and the evolution of complexity. J Mol Evol 33(1):34–41
Pines J (1995) Cyclins and cyclin-dependent kinases: theme and variations. Advances in Cancer Research 66:181–212
Sambrook J, Fritsch E F, Maniatis T (1989) Molecular Cloning: A Laboratory Manual Cold Spring Harbor Laboratory Press, Cold Spring Harbor
Scott M P, Weiner A J (1984) Structural relationships among genes that control development: Sequence homology between the Antennapedia, Ultrahithorax, and fushi tarazu loci of Drosophila. Proc Natl Acad Sci USA 81:4115–4119
Stone B, Wharton W (1994) Targeted RNA fingerprinting: the cloning of differentially-expressed cDNA fragments enriched for members of the zinc finger gene family. Nucleic Acids Res 22:2612–2618
Welsh J, Chada K, Dalai S S, Cheng R, Ralph D, McClelland M (1992) Arbitrarily primed PCR fingerprinting of RNA. Nucleic Acids Res 20:4965–4970
Yoshikawa T, Xing G, Detera-Wadleigh S D (1995) Detection, simultaneous display and direct sequencing of multiple nuclear hormone receptor genes using bilaterally targeted RNA fingerprinting. Biochim Biophys Acta 1264:63–71
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Stone, B. (1997). Targeted RNA Fingerprinting. In: Micheli, M.R., Bova, R. (eds) Fingerprinting Methods Based on Arbitrarily Primed PCR. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60441-6_38
Download citation
DOI: https://doi.org/10.1007/978-3-642-60441-6_38
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-47812-3
Online ISBN: 978-3-642-60441-6
eBook Packages: Springer Book Archive