Summary
A gene library of total DNA of A. brasilense Sp7 was constructed in the broad host range cosmid vector pVKlOO. A plasmid, designated pAB44, that restored by complementation a Gln+ Nif+ phenotype to the Gln- Nif- A. brasilense mutant 7029, was isolated. Plasmid pAB44 contained two HindIII fragments of 20 and 10 kb. The glnA gene was localized on the 20 kb fragment by Tn5 insertion and DNA hybridization with a K. pneumoniae glnA specific probe. Subclones of the 20 kb fragment were obtained in pBR322. No expression of pAB44 was found in E. coli or K. pneumoniae Gln- mutants. However, plasmid mutants which restored glutamine independent growth were obtained in E. coli strain ET8051, that carries a glnAntrBC deletion. The glutamine synthetase was also characterized in E. coli ET8051 crude extracts.
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Fogher, C., Bozouklian, H., Bandhari, S.K., Elmerich, C. (1985). Construction of a Genomic Library of Azospirillum Brasilense SP7 and Cloning of the Glutamine Synthetase Gene. In: Klingmüller, W. (eds) Azospirillum III. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70791-9_5
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DOI: https://doi.org/10.1007/978-3-642-70791-9_5
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