Abstract
The catalytic a subunit of Na+/K+-ATPase (EC 3.6.1.3.) exists in at least three isoforms and contains the binding site for ouabain at the external side of the membrane. Analysis of the primary sequence of this subunit led to the proposal that it presents 4–5 extracellular domains, some implicated in ouabain binding (4, 5). Polyclonal antibodies raised against synthetic peptides in non-homologous regions of the specific a isoform are useful to delineate sequences of importance in ouabain binding, as well as determining the sidedness of the C-terminus (1,3).
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References
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© 1994 Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt
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Antonelli, M.C., Lieste, M.C., Saredi, A., Malchiodi, E., Stahl, W.L. (1994). Polyclonal Antibodies to Extramembrane Domains of Na+/K+-ATPase α1 and α3 Isoforms. In: Bamberg, E., Schoner, W. (eds) The Sodium Pump. Steinkopff. https://doi.org/10.1007/978-3-642-72511-1_38
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DOI: https://doi.org/10.1007/978-3-642-72511-1_38
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