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Analysis of the Leukemogenic Properties of the bcr-v-abl and GM-CSF Genes Using a New Retroviral Vector

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Mechanisms in Myeloid Tumorigenesis 1988

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 149))

Abstract

The haemopoietic system consists of a hierarchy of cells that are produced throughout life from a small number of self renewing stem cells. Many of the molecules that regulate normal haemopoiesis have been defined and characterized by studying aspects of haemopoiesis that can occur in vitro under controlled conditions. Furthermore, our knowledge of the haemopoietic system has been supplemented by investigations of disease phenomena where haemopoiesis is perturbed. These include naturally occurring leukaemias in humans and those induced experimentally in laboratory animals.

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References

  • Bowtell DDL, Johnson GR, Kelso A, Cory S (1987) Expression of genes transferred to haemopoietic stem cells by recombinant retroviruses. Mol Biol Med 4:229–250

    PubMed  CAS  Google Scholar 

  • Bowtell DDL, Cory S, Johnson GR, Gonda TJ (1988) A comparison of expression in hemopoietic cells by retroviral vectors carrying two genes. J Virol 62:2464–2473

    PubMed  CAS  Google Scholar 

  • Cook WD, Metcalf D, Nicola NA, Burgess AW, Walker F (1985) Malignant transformation of a growth factor-dependent myeloid cell line by Abelson virus without evidence of an autocrine mechanism. Cell 41:677–683.

    Article  PubMed  CAS  Google Scholar 

  • Daley GQ, McLaughlin J, Witte ON, Baltimore D (1987) The CML-specific P210 bcr/abl protein, unlike v-abl does not transform NIH/3T3 fibroblasts. Science 237:532–535

    Article  PubMed  CAS  Google Scholar 

  • Dexter TM, Garland J, Scott D, Scolnick E, Metcalf D (1980) Growth of factor-dependent hemopoietic precursor cell lines. J Exp Med 152:1036–1047.

    Article  PubMed  CAS  Google Scholar 

  • Duhrsen U (1988) In vitro growth patterns and autocrine production of hemopoietic colony stimulating factors: analysis of leukemic populations arising in irradiated mice from cells of an injected factor-dependent continuous cell line. Leukemia 2:334–342

    PubMed  CAS  Google Scholar 

  • Gough NM, Grail D, Gearing DP, Metcalf D (1987) Mutagenesis of murine granulocyte-macrophage colony stimulating factor reveals critical residues near the N-terminus. Eur J Biochem 169:353–358

    Article  PubMed  CAS  Google Scholar 

  • Grosveld G, Verwoerd T, Van Agthoven T, De Klein A, Ramachandran KL, Heisterkamp N, Stam K, Groffen J (1986) The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript. Mol Cell Biol 6:607–616

    PubMed  CAS  Google Scholar 

  • Hapel AJ, Lee JC, Farrar WL, Ihle JN (1981) Different colony stimulating factors are detected by the IL-3 dependent cell lines FDC-P1 and 32Dcl-23. Blood 46:786–790.

    Google Scholar 

  • Hariharan IK, Adams JM (1987) cDNA sequence for human bcr, the gene that translocates to the abl oncogene in chronic myeloid leukaemia EMBO J 6:115–119

    PubMed  CAS  Google Scholar 

  • Hariharan IK, Adams JM, Cory S bcr-v-abl oncogene renders myeloid cell line factor independent: potential autocrine mechanism in chronic myeloid leukaemia. (submitted)

    Google Scholar 

  • Konopka JB, Watanabe SM, Witte ON (1984) An alteration of the human c-abl protein in K562 leukemia cells unmasks associated tyrosine kinase activity. Cell 37:1035–1042

    Article  PubMed  CAS  Google Scholar 

  • Lang RA, Matcalf D, Gough NM, Dunn AR, Gonda TJ (1985) Expression of a hemopoietic growth factor cDNA in a factor-dependent cell line results in autonomous growth and tumourigenicity. Cell 33:153–159

    Google Scholar 

  • Mann R, Mulligan RC, Baltimore D (1983) Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus. Cell 33:153–159

    Article  PubMed  CAS  Google Scholar 

  • Metcalf D (1985) The granulocyte-macrophage colony-stimulating factors. Science 229:16–22

    Article  PubMed  CAS  Google Scholar 

  • Oppi C, Shore SK, Reddy EP (1987) Nucleotide sequence of testis-derived c-abl cDNAs: implications for testis-specific transcription and abl oncogene activation. Proc. Natl. Acad. Sci. USA 84:8200–8204

    Article  PubMed  CAS  Google Scholar 

  • Shaw G, Kamen R (1986) A conserved AU sequence from the 3’ untranslated region of GM-CSF mRNA mediates selective mRNA degradation. Cell 46:659–667.

    Article  PubMed  CAS  Google Scholar 

  • Shtivelman E, Lifshitz B, Gale RP, Canaani E (1985) Fused transcript of abl and bcr genes in chronic myelogenous leukaemia. Nature 315:550–554

    Article  PubMed  CAS  Google Scholar 

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© 1989 Springer-Verlag Berlin · Heidelberg

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Hariharan, I.K., Johnson, G.R., Gonda, T.J., Metcalf, D., Adams, J.M., Cory, S. (1989). Analysis of the Leukemogenic Properties of the bcr-v-abl and GM-CSF Genes Using a New Retroviral Vector. In: Shen-Ong, G.L.C., Potter, M., Copeland, N.G. (eds) Mechanisms in Myeloid Tumorigenesis 1988. Current Topics in Microbiology and Immunology, vol 149. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74623-9_9

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  • DOI: https://doi.org/10.1007/978-3-642-74623-9_9

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-74625-3

  • Online ISBN: 978-3-642-74623-9

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