Summary
Human chondrocytes growing in monolayer cultures de-differentiate and produce type I collagen. They re-differentiate and resume their in-vivo characteristics (including the production of type II collagen) when cultured in an agarose-gel. To characterize the modulated cells in more detail, biochemical studies were performed in chondrocytes suspended in agarose for 1 to 3 weeks.
Glycosaminoglycan (GAG) composition, electrophoretic mobility and hydrodynamic size of proteoglycans were identical in tissue and in cultured cells. These findings suggest that cartilage cells fully regain their functional identity in agarose gel cultures.
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Literatur
Benya PD, Shaffer JD (1982) Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gel. Cell 30: 215–224
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© 1989 Springer-Verlag · Heidelberg
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Beck, M., Aulthouse, A., Horton, W.A. (1989). In-vitro Proteoglykansynthese in redifferenzierten Chondrozyten. In: Willert, HG., Heuck, F.H.W. (eds) Neuere Ergebnisse in der Osteologie. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74770-0_23
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DOI: https://doi.org/10.1007/978-3-642-74770-0_23
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-51175-5
Online ISBN: 978-3-642-74770-0
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