Abstract
Small heat shock protein (hsp) genes of Drosophila are not only activated by heat but also by the steroid hormone ecdysterone. Promoter deletion analysis has revealed that the two stimuli regulate gene expression by distinct mechanisms. Sequence elements that are critically important for ecdysterone activation of the hsp27 and hsp23 genes have been identified and have been used to purify ecdysterone receptor by specific DNA affinity chromatography. Purified receptor enhances transcription in vitro from promoters containing the above sequence elements. Thus, ecdysterone receptor is a bona fide transcription factor and is essential for the hormonal activation of small hsp genes. Recent experiments suggest that an additional factor modifies this regulation.
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References
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© 1991 Springer-Verlag Berlin Heidelberg
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Voellmy, R., Luo, Y., Mestril, R., Amin, J., Ananthan, J. (1991). Mechanisms of Regulation of Small Heat Shock Protein Genes in Drosophila . In: Maresca, B., Lindquist, S. (eds) Heat Shock. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76679-4_4
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DOI: https://doi.org/10.1007/978-3-642-76679-4_4
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