Abstract
Cloned origin-rich sequences (ors) isolated by strand extrusion from mammalian (monkey and human) cells are capable of transient autonomous semiconservative replication in transfected HeLa cells. Sequence analyses reveal no primary consensus sequence features that can be associated with origin function, but several common structural features have been noted which include A/T-rich stretches of DNA and inverted repeats that can potentially give rise to cruciform structures. Some similarities to the yeast ARS consensus, the consensus for attachment to nuclear scaffold and for other regulatory elements have also been noted. The data suggest that origin activation is most probably dependent on the presence of structural determinants rather than on primary sequence.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Decker RS, Yamaguchi M, Possenti R, DePamphilis ML (1986) Initiation of simian virus 40 DNA replication in vitro: aphidicolin causes accumulation of early-replicating intermediates and allows determination of the initial direction of DNA synthesis. Mol. Cell. Biol. 6:3815–3825
Dierks P, Van Ooyen A, Cochran MD, Dobkin C, Reiser J, Weissmann C (1983) Three regions upstream from the cap site are required for efficient and accurate transcription of the rabbit ß-globin gene in mouse 3T6 cells. Cell 32:695–706
Frappier L (1988) Structural and functional analysis of mammalian origins of DNA replication. Ph.D. Thesis, McGill University
Frappier L, Zannis-Hadjopoulos M (1987) Autonomous replication of plasmids bearing monkey DNA origin-enriched sequences. Proc. Natl. Acad. Sci. USA 84:6668–6672
Galson DL, Housman DE (1988) Detection of two tissue-specific DNA binding proteins with affinity for sites in the mouse ß-globin intervening sequence 2. Mol. Cell. Biol. 8:381–392
Gasser SM, Laemmli UK (1986) Cohabitation of scaffold binding regions with upstream/enhancer elements of three developmen-tally regulated genes of D.melanoqaster. Cell 46:521–530
Guo Z-S, Gutierrez C, Heine U, Sogo JM, DePamphilis ML (1989) Origin auxiliary sequences can facilitate initiation of simian virus 40 DNA replication in vitro as they do in vivo. Mol. Cell. Biol. 9:3593–3602
Kaufmann G, Zannis-Hadjopoulos M, Martin RG (1985) Cloning of nascent monkey DNA synthesized early in the cell cycle. Mol. Cell. Biol. 5:721–727
Landry S, Zannis-Hadjopouos M (1991) Classes of autonomously replicating sequences are found among early-replicating monkey DNA. Biochim. Biophys. Acta in press
Palzkill TG, Newlon CS (1988) A yeast replication origin consists of multiple copies of a small conserved sequence. Cell 53:441–450
Rao BS, Zannis-Hadjopoulos M, Price GB, Reitman M, Martin RG (1990) Sequence similarities among monkey ori-enriched (ors) fragments. Gene 87:233–242
Smith CA, Cooper PK, Hanawalt PC (1981) Measurement of repair replication by equilibrium sedimentation. In: DNA repair, a lab manual of research procedures, vol.1, part B, EC Friedman PC Hanawalt (eds), Marcel Dekker, Inc., New York and Basel
Stillman BW, Gluzman Y (1985) Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells. Mol. Cell. Biol. 5:2051–2060
Zannis-Hadjopoulos M, Persico M, Martin RG (1981) The remarkable instability of replication loops provide a general method for the isolation of origins of DNA replication. Cell 27:155–163
Zannis-Hadjopoulos M, Chepelinsky AB, Martin RG (1983) Mapping of the 3′-end positions of simian virus 40 nascent strands. J. Mol. Biol. 165:599–607
Zannis-Hadjopoulos M, Kaufmann G, Martin RG (1984) Mammalian DNA enriched for replication origins is enriched for snap-back sequences. J. Mol. Biol. 179:577–586
Zannis-Hadjopoulos M, Kaufmann G, Wang S.-S, Lechner RL, Karawya E, Hesse J, Martin RG (1985) Properties of some monkey DNA sequences obtained by a procedure that enriches for DNA replication origins. Mol. Cell. Biol. 5:1621–1629
Zannis-Hadjopoulos M, Frappier L, Khoury M, Price GB (1988) Effect of anti-cruciform DNA monoclonal antibodies on DNA replication. The EMBO J. 7:1837–1844
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Zannis-Hadjopoulos, M., Pearson, C.E., Bell, D., Mah, D., McAlear, M., Price, G.B. (1992). Structural and Functional Characteristics of Autonomously Replicating Mammalian Origin-Enriched Sequences (ORS). In: Hughes, P., Fanning, E., Kohiyama, M. (eds) DNA Replication: The Regulatory Mechanisms. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76988-7_10
Download citation
DOI: https://doi.org/10.1007/978-3-642-76988-7_10
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-76990-0
Online ISBN: 978-3-642-76988-7
eBook Packages: Springer Book Archive