Skip to main content
SpringerLink
Log in

Structural Studies on a Ribonucleoprotein Organelle: The Ribosome

  • Chapter
Nucleic Acids and Molecular Biology

Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 6))

  • 222 Accesses

Abstract

One of the most abundant cellular assemblies of RNA and proteins is the ribosome, the organelle which operates as a multi-functional enzyme translating the genetic code into polypeptide chains. Ribosomes are built of two structurally independent subunits of unequal size, which associate upon initiation of protein biosynthesis. A typical bacterial ribosome contains about a quarter of a million atoms and is of a molecular weight of approximately 2.3 million daltons. About two-thirds of its mass is comprised of three chains of rRNA, the rest includes some 57 different proteins.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Abdul-Meguid SS, Moore PB, Steitz TA (1983) Crystallization of a ribonuclease-resistant fragment of E. coli 5S ribosomal RNA and its complex with protein 125. J Mol Biol 171: 207–215

    Article  Google Scholar 

  • Arad T, Leonard KR, Wittmann HG, Yonath A (1984) Two-dimensional crystalline sheets of B. stearothermophilus ribosomal particles. EMBO J 3: 127–131

    PubMed  CAS  Google Scholar 

  • Arad T, Piefke J, Gewitz HS, Hennemann B, Glotz C, Müssig J, Yonath A, Wittmann HG (1987a) The growth of ordered two-dimensional sheets of ribosomal particles from salt-alcohol mixtures. J Anal Biochem 167: 113–117

    Article  CAS  Google Scholar 

  • Arad T, Piefke J, Weinstein S, Gewitz HS, Yonath A, Wittmann HG (1987b) Three-dimensional image reconstruction from ordered arrays of 70S ribosomes. Biochimie 69: 1001–1005

    Article  PubMed  CAS  Google Scholar 

  • Barnebeu C, Lake JA (1982) Nascent polypeptide chains emerge from the exit domain of the large ribosomal subunit: immune mapping of the nascent chain. Proc Nati Acad Sci USA 79: 3111–3115

    Article  Google Scholar 

  • Bergman U, Arndt E (1990) Evidence for an additional archaebacterial gene cluster in Halobacterium marismortui encoding ribosomal proteins HL46e and HL30. Biochem Biophys Acta 1050: 56–60

    Google Scholar 

  • Berkovitch-Yellin Z, Wittmann HG, Yonath A (1990) Low resolution models for ribosomal particles reconstructed from electron micrographs of tilted two-dimensional sheets: tentative assignments of functional sites. Acta Crystallogr B46: 637–643

    Article  Google Scholar 

  • Berkovitch-Yellin Z, Hansen H, Bennett WS, Sharon R, von Böhlen K, Volkmann N, Piefke J, Yonath A, Wittmann HG (1991a) Crystals of 70S ribosomes from thermophilic bacteria are suitable for X-ray analysis at low resolution, J Crystal Growth 110: 208–213

    Article  CAS  Google Scholar 

  • Berkovitch-Yellin Z, Bennett WS, Yonath A (1991b) Aspects in structural studies on ribosomes. CRC Rev Bioch (in press)

    Google Scholar 

  • Blobel G, Sabatini DD (1970) Controlled proteolysis of nascent polypeptides in rat liver cell fractions. J Cell Biol 45: 130–145

    Article  PubMed  CAS  Google Scholar 

  • Boeckh T, Wittmann HG (1991) Synthesis of a radioactive labeled undecagold cluster for application in X-ray structure analysis of ribosomes. Biochem Biophys Acta 1075: 50–55

    Article  PubMed  CAS  Google Scholar 

  • Brimacombe R, Amadja J, Stiege W, Schueler D (1988) A detailed model of the three-dimensional structure of E. coli 16S ribosomal RNA in situ in the 30S subunit. J Mol Biol 199: 115–136

    Article  PubMed  CAS  Google Scholar 

  • Brimacombe R, Greuer B, Mitcell P, Osswald M, Rinke-Appel J, Schueler, D. Stade K (1990) Three-dimensional structure and function of E. coli 16S and 23S rRNA as studied by cross-linking techniques. In: Hill EW, Dahlberg A, Garrett RA, Moore PB, Schlesinger D, Warner JR (eds) The ribosomes, structure, function and evolution. Am Soc Microbiol, Washington, pp 93–106

    Google Scholar 

  • Clark W, Leonard KR, Lak J (1982) Ribosomal crystalline arrays of large subunits from E. coli. Science 216: 999–1000

    Article  PubMed  CAS  Google Scholar 

  • Cunnigham PR, Weizmann CJ, Nrgre D, Sinning JG, Frick V, Nurse K, Ofengand J (1990) In vitro analysis of the role of rRNA in protein biosynthesis: site specific mutation and methylation. In: Hill EW, Dahlberg A, Garrett RA, Moore PB, Schlesinger D, Warner JR (eds) The ribosomes, structure, function and evolution. Am Soc Microbiol, Washington, pp 243–252

    Google Scholar 

  • Egebjerg J, Larsen N, Garrett RA (1990) Structural map of 23S rRNA. In: Hill EW, Dahlberg A, Garrett RA, Moore PB, Schlesinger D, Warner JR (eds) The ribosomes, structure, function and evolution. Am Soc Microbiol, Washington, pp 168–179

    Google Scholar 

  • Ehreshmann B, Ehreshmann C, Romby P, Mougel M, Baudin F, Westhof E, Ebel JP (1990) Detailed structure of rRNA: new approaches. In: Hill EW, Dahlberg A, Garrett RA, Moore PB, Schlesinger D, Warner JR (eds) The ribosomes, structure, function and evolution. Am Soc Microbiol, Washington, pp 148–159

    Google Scholar 

  • Eisenstein M, Sharon R, Berkovitch-Yellin Z, Gewitz HS, Weinstein S, Pebay-Peyroula E, Roth M, Yonath A (1991) The interplay between X-ray crystallography, neutron diffraction, image reconstruction, organo-metallic chemistry and biochemistry in structural studies of ribosomes. Biochemie 73: 879–886

    Article  CAS  Google Scholar 

  • Evers U, Gewitz HS (1989) Studies on the accessibility of nascent non-helical peptide chains on the ribosomes. Biochem Int 19: 1031–1038

    PubMed  CAS  Google Scholar 

  • Frank J, Verschoor A, Radamaacher M, Wagenknecht T (1990) Morphologies of eubacterial and eukaryotic ribosomes as determined by three dimensional electron microscopy. In: Hill EW, Dahlberg A, Garrett RA, Moore PB, Schlesinger D, Warner JR (eds) The ribosomes, structure, function and evolution. Am Soc Microbiol, Washington, pp 107–113

    Google Scholar 

  • Gewitz HS, Glotz C, Piefke J, Yonath A, Wittmann HG (1988) Two-dimensional crystalline sheets of the large ribosomal subunits containing the nascent protein chain. Biochimie 70: 645–648

    Article  PubMed  CAS  Google Scholar 

  • Gilbert W (1963) Protein synthesis in E. coli. Cold Spring Harbor Symp Quant Biol 28: 287–294

    CAS  Google Scholar 

  • Glotz C, Müssig J, Gewitz HS, Makowski I, Arad T, Yonath A, Wittmann HG (1987) Three-dimensional crystals of ribosomes and their subunits from eu-and archaebacteria. Biochem Int 15: 953–960

    PubMed  CAS  Google Scholar 

  • Hansen H, Volkmann N, Piefke J, Glotz C, Weinstein S, Makowski I, Meyer S, Wittmann HG, Yonath A (1990) Crystals of complexes mimicking protein biosynthesis are suitable for crystallographic studies. Biochim Biophys Acta 1050: 1–8

    PubMed  CAS  Google Scholar 

  • Hardesty B, Kramer G (1986) Structure,’function and genetics of ribosomes. Springer, Berlin Heidelberg New York

    Book  Google Scholar 

  • Hardesty B, Picking WD, Odom OW (1990) The extension of polyphenyl alanynine and polylysine peptides on E. coli ribosomes. Biochim Biophys Acta 1050: 197–202

    PubMed  CAS  Google Scholar 

  • Hill WE, Trappich BE, Tassanakajohn B (1986) Probing ribosomal structure and function. In: Hardesty B, Kramer G (eds) Structure, function and genetics of ribosomes. Springer, Berlin Heidelberg New York, pp 233–256

    Chapter  Google Scholar 

  • Hill EW, Dahlberg A, Garrett RA, Moore PB, Schlesinger D, Warner JR (eds) (1990a) The ribosomes: structure, function and evolution. Am Soc Microbiol, Washington

    Google Scholar 

  • Hill WE, Weller J, Gluick T, Merryman C, Marconi RT, Tassanakajohn A, Tappich WE (1990b) Probing ribosome structure and function by using short complementary DNA oligomers. In: Hill EW, Dahlberg A, Garrett RA, Moore PB, Schlesinger D, Warner JR (eds) The ribosomes, structure, function and evolution. Am Soc Microbiol, Washington, pp 253–264

    Google Scholar 

  • Hope H, Frolow F, von Böhlen K, Makowski I, Kratky C, Halfon Y, Danz H, Webster P, Bartels K, Wittmann HG, Yonath A (1989) Cryocrystallography of ribosomal particles. Acta Crystallogr B45 (345): 190–199

    Google Scholar 

  • Jahn W (1989a) Synthesis of water soluble undecagold cluster for specific labeling of proteins. Z Naturforsch 44b: 1313–1322

    CAS  Google Scholar 

  • Jahn W (1989b) Synthesis of water soluble tetrairidium clusters suitable for heavy atom labeling of proteins. Z Naturforsch 44b: 79–82

    CAS  Google Scholar 

  • Jurnak FA, McPherson A (eds) (1984) Biological macromolecules and assemblies I. Virus structures. John Wiley & Sons, New York

    Google Scholar 

  • Kang C, Cantor CR (1985) Structure of ribosome bound mRNA as revealed by enzymatic accessibility studies. J Mol Biol 210: 659–663

    Google Scholar 

  • Klug A, Holmes KC, Finch JT (1961) X-ray diffraction studies on ribosomes from different sources. J Mol Biol 3: 87–100

    Article  PubMed  CAS  Google Scholar 

  • Koepke AKE, Paulke C, Gewitz HS (1990) Overexpression of methanococcal ribosomal protein L12 in E. colt and its incorporation into halobacterial 505 subunits yielding active ribosomes, J Biol Chem 265: 6436–64

    CAS  Google Scholar 

  • Kolb VA, Kommer A, Spirin AS (1990) Nascent peptide and the ribosomal tunnel. Worksh translation, Leiden, p 84a

    Google Scholar 

  • Kress Y, Wittner M, Rosenbaum RM (1971) Sites of cytoplasmic ribonucleoproteinfilament assembly in relation to helical body formation in axenic trophozoites of Entamoeba histolytica. J Cell Biol 49: 773–784

    Article  PubMed  Google Scholar 

  • Kühlbrandt W, Unwin PNT (1982) Distribution of RNA and proteins in crystalline eukaryotic ribosomes. J Mol Biol 156: 431–488

    Article  PubMed  Google Scholar 

  • Kurzchalia SV, Wiedmann M, Breter H, Zimmermann W, Bauschke E, Rapoport TA (1988) tRNA-mediated labeling of proteins with biotin, a nonradioactive method for the detection of cell-free translation products. Eur J Biochem 172: 663–668

    Google Scholar 

  • Lake JA (1979) Ribosome structural ad functional sites. In: Chambliss G, Craven GR, Davies J, Davis K, Kahan L, Nomura M (eds) Ribosomes structure, function and genetics. University Park Press, Baltimore, pp 207–236

    Google Scholar 

  • Lake JA, Slayter H (1972) Three-dimensional structure of the chromatoid body helix of entamoeba invades. J Mol Biol 66: 271–282

    Article  PubMed  CAS  Google Scholar 

  • Langridge R, Holmes KC (1962) X-ray diffraction studies of concentrated gels of ribosomes from E. coli. J Mol Biol 5: 611–618

    Article  CAS  Google Scholar 

  • Leonard KR, Arad T, Tesche B, Erdmann VA, Wittmann HG, Yonath A (1982) Crystallization, electron microscopy and three-dimensional reconstruction studies of ribosomal subunits. In: Le Poole JB, Zeitler E, Thomas G, Schimmel G, Weichman C, Bassewitz KV (eds) Electron microscopy 1982, vol 3. Hartung, Hamburg, pp 915

    Google Scholar 

  • Lorenz S, Betzel C, Raderschall E, Dauter Z, Wilson KS, Erdmann VA (1991) Crystallization and preliminary diffraction studies of 5S RNA from the thermophilic bacterium Thermus flavus. J Mol Biol 219: 390–402

    Google Scholar 

  • Makowski I, Frolow F, Saper MA, Wittmann HG, Yonath A (1987) Single crystals of large ribosomal particles from Halobacterium marismortui diffract to 6 A. J Mol Biol 193: 819–821

    Article  PubMed  CAS  Google Scholar 

  • Malkin LI, Rich A (1967) Partial resistance of nascent polypeptide chains to proteolytic digestion due to ribosomal shielding. J Mol Biol 26: 329–346

    Article  PubMed  CAS  Google Scholar 

  • Milligan RA, Unwin PNT (1982) In vitro crystallization of ribosomes from chick embryos. J Cell Biol 95: 648–652

    Article  PubMed  CAS  Google Scholar 

  • Milligan RA, Unwin PNT (1986) Location of the exit channel for nascent proteins in 80S ribosomes. Nature (London) 319: 693–696

    Article  CAS  Google Scholar 

  • Moore P (1988) The ribosome returns. Nature (London) 331: 223–229

    Article  CAS  Google Scholar 

  • Moras D (1989) Crystal structure of tRNAs. In: Saenger W (ed) Landolt-Börnstein, new ser lb. Nucleic acids. Springer, Berlin Heidelberg New York, pp 1–30

    Google Scholar 

  • Morikawa K, Kawakami M, Takenura S (1982) Crystallization and Preliminary diffraction studies of 5S RNA from Thermus thermophilus HB8. FEBS Lett 145: 194–196

    Article  PubMed  CAS  Google Scholar 

  • Müssig J, Makowski I, von Böhlen K, Hansen H, Bartels KS, Wittmann HG, Yonath A (1989) Crystals of wild-type, mutated, derivatized and complexed 505 ribosomal subunits from Bacillus stearothermophilus suitable for X-ray analysis. J Mol Biol 205: 619–621

    Article  PubMed  Google Scholar 

  • Noller HF (1991) Ribosomal RNA and translation. Annu Rev Biochem 60: 191–227

    Article  PubMed  CAS  Google Scholar 

  • Noller HF, Moazed D, Stern S, Powers T, Allen PN, Robertson BW, Triman K (1990) In: Hill EW, Dahlberg A, Garrett RA, Moore PB, Schlesinger D, Warner JR (eds) The ribosomes, structure, function and evolution. Am Soc Microbiol, Washington, pp 73–92

    Google Scholar 

  • Oakes M, Henderson E, Scheiman A, Clark M, Lake J (1986a) Ribosome structure, function and evolution: mapping ribosomal RNA, proteins and functional site in three dimensions. In: Hardesty B, Kramer G (eds) Structure, function and genetics of ribosomes. Springer, Berlin Heidelberg New York, pp 47–67

    Chapter  Google Scholar 

  • Oakes M, Clarck M, Henderson E, Lake J (1986b) DNA hybridization electron microscopy: ribosomal RNA nucleotides 1392–1407 are exposed in the cleft of the small subunit. Proc Natl Acad Sci USA 83: 275–918

    Article  PubMed  CAS  Google Scholar 

  • Oakes M, Scheiman A, Atha T, Shakweiler G, Lake J (1990) Ribosome structure: three-dimensional location of rRNA and proteins. In: Hill EW, Dahlberg A, Garrett RA, Moore PB, Schlesinger D, Warner JR (eds) The ribosomes, structure, function and evolution. Am Soc Microbiol, Washington, pp 180–193

    Google Scholar 

  • O’Brien L, Shelley K, Towfighi J, McPherson A (1980) Crystalline ribosomes are present in brain of senile humans. Proc Natl Acad Sci USA 77: 2260–2264

    Article  PubMed  Google Scholar 

  • Raue HA, Munster W, Rutgers CA, Riet JV, Planta R (1990) rRNA: from structure to function. In: Hill EW, Dahlberg A, Garrett RA, Moore PB, Schlesinger D, Warner JR (eds) The ribosomes, structure, function and evolution. Am Soc Microbiol, Washington, pp 217–235

    Google Scholar 

  • Rheinberger HJ, Nierhaus KH (1990) Partial release of AcPhe2-tRNA from ribosome during poly(U) dependent poly(phe) synthesis and the effects of chloramphenicol. Eur J Biochem 193: 643–650

    Article  PubMed  CAS  Google Scholar 

  • Rinke-Apple J, Jünke N, Stade K, Brimacombe R (1991) The path of mRNA through E. coli ribosome, site directed cross-linking of mRNA analogues carrying a photo-reactive label at various points 3’ to the decoding site. EMBO J 10: 2195–2202

    Google Scholar 

  • Rould MA, Perona JJ, Soell D, Steitz TA (1989) Structure of E. coli glutamyl-tRNA synthetase complexed with tRNAg and ATP at 2.8 A resolution: implications for tRNA discrimination Science 246: 1135–1142

    Article  PubMed  CAS  Google Scholar 

  • Rould MA, Steitz TA (1992) Structure of the synthetase-tRNA glu-ATP complex. In: Eckstein F, Hilley, DM (eds) Biology, Vol. 6, Springer, Berlin Heidelberg pp 254

    Google Scholar 

  • Ruff M, Krishnaswamy S, Boeglin M, Poterszman A, Mitchler A, Podjarny A, Moras D (1991) Class II aminoacyl tRNA synthetases: crystal structure of yeast aspartyl-tRNA synthetase complexed with tRNAasP. Science 252: 1682–1689

    Article  PubMed  CAS  Google Scholar 

  • Ryabova LA, Selivanova OM, Baranov VI, Vasiliev VD, Spirin AS (1988) Does the channel for nascent peptide exist inside the ribosome? FEBS Lett 226: 255–260

    Article  PubMed  CAS  Google Scholar 

  • Sanchez ME, Urena D, Amils R, Londei P (1990) In vitro reassembly of active large ribosomal subunits of the halophilic archaebacterium Halogax mediterrane, Biochemistry 29: 9256–9259

    Article  PubMed  CAS  Google Scholar 

  • Schnier J, Gewitz HS, Behrens E, Lee A, Ginther G, Leighton T (1990) Isolation and characterization of Bacillus stearothermophilus 30S and 50S ribosomal protein mutations. J Bacteriol 172: 7306–7309

    PubMed  CAS  Google Scholar 

  • Shevack A, Gewitz HS, Hennemann B, Yonath A, Wittmann HG (1985) Characterization and crystallization of ribosomal particles from Halobacterium marismotoui. FEBS Lett 184: 68–71

    Article  CAS  Google Scholar 

  • Smith WP, Tai PC, Davis BD (1978) Interaction of secreted nascent chains with surrounding membranes in Bacillus subtilis. Proc Natl Acad Sci USA 75: 5922–5925

    Article  PubMed  CAS  Google Scholar 

  • Spirin A (1987) Structural dynamic aspects of protein synthesis on ribosomes. FEBS Lett 69: 949–956

    CAS  Google Scholar 

  • Tappich WE, Goerringer HU, De Stasio E, Prescott C, Dahlenberg AE (1990) Studies on ribosome function by mutagenesis of E. coli rRNA In: Hill EW, Dahlberg A, Garrett RA, Moore PB, Schlesinger D, Warner JR (eds) The ribosomes, structure, function and evolution. Am Soc Microbiol, Washington, pp 236–243

    Google Scholar 

  • Trackhanov SD, Yusupov MM, Agalarov SC, Garber MB, Ryazantsev SN, Tischenko SV, Shirokov VA (1987) Crystallization of 70S ribosomes and 30S ribosomal subunits from Thermus thermophilus. FEBS Lett 220: 319–322

    Article  Google Scholar 

  • Trackhanov SD, Yusupov MM, Shirokov VA, Garber MB, Mitschler A, Ruff M, Thierry JC, Moras D (1989) Preliminary X-ray investigation of 70S ribosome crystals from Thermus thermophilus. J Mol Biol 209: 327–331

    Article  Google Scholar 

  • Unwin PNT (1979) Attachment of ribosome crystals to intracellular membranes. J Mol Biol 132: 69–79

    Article  PubMed  CAS  Google Scholar 

  • Unwin PNT, Taddei C (1977) Packing of ribosomes in crystals from the lizard Lacerta sicula. J Mol Biol 114: 491–499

    Article  PubMed  CAS  Google Scholar 

  • Volkmann N, Hottentrager S, Hansen H, Zayzsev-Bashan A, Sharon R, Berkovitch-Yellin Z, Yonath A, Wittmann HG (1990) Characterization and preliminary crystallographic studies on large ribosomal subunits from Thermus thermophilus. J Mol Biol 216: 239–241

    Article  PubMed  CAS  Google Scholar 

  • Von Böhlen K, Makowski I, Hansen HAS, Bartels H, Berkovitch-Yellin Z, Zaytzev-Bashan A, Meyer S, Paulke C, Franceschi F, Yonath A (1991) Single crystals of large ribosomal particles from Halobacterium marismortui diffract to 3 A. J Mol Biol 222: 11–15

    Article  Google Scholar 

  • Wagenknecht T, Graassucc R, Frank J (1989) Electron microscopy and computer image averaging of ice-embedded large ribosomal subunits from E. coli. J Mol Biol 199: 137–147

    Google Scholar 

  • Weinstein S, Jahn W, Hansen HAS, Wittmann HG, Yonath A (1989) Novel procedures of derivatization of ribosomes for crystallographic studies. J Biol Chem 264: 19138–19142

    PubMed  CAS  Google Scholar 

  • Wittmann HG (1983) Architecture of prokaryotic ribosomes. Annu Rev Biochem 52: 35–65

    Article  PubMed  CAS  Google Scholar 

  • Wittmann HG, Yonath A (1985) Diffraction studies on crystals of ribosomal particles. In: Ts’o POP, Nicollini C (eds) The structure and function of genetic apparatus. Plenum, New York, pp 177–189

    Google Scholar 

  • Wittmann HG, Müssig J, Gewitz HS, Piefke J, Rheinberger HJ, Yonath A (1982) Crystallization of E. coli ribosomes. FEBS Lett 146: 217–220

    Article  PubMed  CAS  Google Scholar 

  • Yen IJ, Macklin PS, Cleavland DW (1988) Autoregulated instability of beta-tubulin mRNAs by recognition of the nascent amino terminus of beta-tubulin. Nature (London) 334: 580–585

    Article  CAS  Google Scholar 

  • Yonath A (1984) Three-dimensional crystals of ribosomal particles. TIBS 9: 227–230

    Google Scholar 

  • Yonath A, Wittmann HG (1988) Approaching the molecular structure of ribosomes. J Biophys Chem 29: 17–29

    Article  CAS  Google Scholar 

  • Yonath A, Wittmann HG (1989a) Challenging the three-dimensional structure of ribosomes. TIBS 14: 329–335

    PubMed  CAS  Google Scholar 

  • Yonath A, Wittmann HG (1989b) Crystallographic and image reconstruction studies on ribosomal particles from bacterial sources. Meth Enzymol 164: 95–117

    Article  Google Scholar 

  • Yonath A, Müssig J, Tesche B, Lorenz S, Erdmann VA, Wittmann HG (1980) Crystallization of the large ribosomal subunit from B. stearothermophilus. Biochem Int 1: 428–435

    CAS  Google Scholar 

  • Yonath A, Müssig J, Wittmann HG (1982a) Parameters for crystal growth of ribosomal subunits. J Cell Biochem 19: 145–155

    Article  PubMed  CAS  Google Scholar 

  • Yonath A, Khavitch G, Tesche B, Müssig J, Lorenz S, Erdmann VA, Wittmann HG (1982b) The nucleation of crystals of the large ribosomal subunits from B. stearothermophilus. Biochem Int 5: 629–639

    CAS  Google Scholar 

  • Yonath A, Saper MA, Makowski I, Müssig J, Piefke J, Bartunik HD, Bartels KS, Wittmann HG (1986a) Characteriation of single crystals of the large ribosomal particles from B. stearothermophilus. J Mol Biol 187: 633–636

    Article  PubMed  CAS  Google Scholar 

  • Yonath A, Saper MA, Wittmann HG (1986b) Structural studies on ribosomal particles. In: Hardesty B, Kramer G (eds) Structure, function and genetics of ribosomes. Springer, Berlin Heidelberg New York, pp 112–129

    Chapter  Google Scholar 

  • Yonath A, Saper MA, Frolow F, Makowski I, Wittmann HG (1986c) Characterization of single crystals of large ribosomal particles from a mutant of B. stearothermophilus. J Mol Biol 192: 161–162

    Article  PubMed  CAS  Google Scholar 

  • Yonath A, Leonard KR, Wittman HG (1987a) A tunnel in the large ribosomal subunit revealed by three-dimensional image reconstruction. Science 236: 813–816

    Article  PubMed  CAS  Google Scholar 

  • Yonath A, Leonard KR, Weinstein S, Wittmann HG (1987b) Approaches to the determination of the three-dimensional architecture of ribosomal particles. Cold Spring Harbor Symp Quant Biol 52: 729–741

    PubMed  CAS  Google Scholar 

  • Yonath A, Glotz C, Gewitz HS, Bartels K, von Böhlen K, Makowski I, Wittmann HG (1988) Characterization of crystals of small ribosomal subunits. J Mol Biol 203: 831–834

    Article  PubMed  CAS  Google Scholar 

  • Yonath A, Bennett W, Weinstein W, Wittmann HG (1990) Crystallography and image reconstruction of ribosomes. In: Hill EW, Dahlberg A, Garrett RA, Moore PB, Schlesinger D, Warner JR (eds) The ribosomes, structure, function and evolution. Am Soc Microbiol, Washington, pp 134–147

    Google Scholar 

  • Yusupov MM, Tischenko SV, Trackhanov SD, Ryazantsev SN, Garber MB (1988) A new crystalline form of 30S ribosomal subunits from Thermus thermophilus. FEBS Lett 238, 113: 115

    Google Scholar 

  • Zamir A (1977) Affinity Labeling of ribosomal functional sites. Meth Enzymol 46: 621–711

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Structural Chemistry, Weizmann Institute, Rehovot, Israel

    A. Yonath

  2. Max-Planck-Research-Unit for Ribosomal Structure, c/o DESY, Nötkestraße 85, W-2000, Hamburg, Germany

    A. Yonath

Authors
  1. A. Yonath
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Max-Planck-Institut für Experimentelle Medizin, Hermann-Rein-Straße 3, W-3400, Göttingen, Germany

    Fritz Eckstein

  2. Biochemistry Department, University of Dundee, DD1 4HN, Dundee, UK

    David M. J. Lilley

Rights and permissions

Reprints and permissions

Copyright information

© 1992 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Yonath, A. (1992). Structural Studies on a Ribonucleoprotein Organelle: The Ribosome. In: Eckstein, F., Lilley, D.M.J. (eds) Nucleic Acids and Molecular Biology. Nucleic Acids and Molecular Biology, vol 6. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77356-3_14

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-77356-3_14

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-77358-7

  • Online ISBN: 978-3-642-77356-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation