Abstract
Because of the complexity and the vast number of metabolic and other physiologic functions provided by the liver, it was felt that in constructing an extracorporeal liver support system, viable, isolated, intact hepatocytes should be utilized rather than specific cell components or enzymes. Previously, Reid and Rojkind [1] demonstrated that isolated hepatocyte viability and expression of differentiated function in vitro were enhanced when the hepatocytes were maintained as a monolayer on a collagen biomatrix; several types of biomatrix have been described for maintaining primary hepatocyte cultures for long periods of time. We hypothesized that “anchored” hepatocytes attached to a collagen matrix would be better able to express differentiated liver functions in an extracorporeal liver support system because of the more physiologic environment which would allow cell-cell and cell-matrix interactions.
Supported by grants from the National Institutes of Health (DK 38763) and the Veterans Administration (Merit Review Award).
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© 1992 Springer-Verlag Berlin Heidelberg
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Demetriou, A.A., Arnaout, W.S., Backfisch, G., Moscioni, A.D. (1992). Immobilized Isolated Liver Cells on a Biomatrix. In: Brunner, G., Mito, M. (eds) Artificial Liver Support. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77359-4_23
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DOI: https://doi.org/10.1007/978-3-642-77359-4_23
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