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Oligo-[α]-Deoxyribonucleotides Covalently Linked to Intercalating Agents. A New Family of Sequence-Specific Nucleic Acid Reagents

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Nucleic Acids and Molecular Biology

Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 2))

Abstract

During the last few years, there has been a growing interest in using oligo-nucleotides to specifically regulate gene expression (Knorre and Vlassov 1985; Green et al. 1986). An oligonucleotide can be targeted to a messenger RNA. Complex formation via hydrogen bonding interactions (base pair formation) is expected to interfere with mRNA processing or translation and consequently inhibits protein synthesis. When targeted to a viral RNA the oligonucleotide might inhibit different processes depending on the nature of the virus (protein synthesis, RNA replication, etc.). During DNA transcription and replication, the double helix is transiently and locally opened by polymerases. single-stranded regions are made accessible to oligonucleotides which might interfere with the normal course of the enzymatic processes.

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Authors and Affiliations

  1. Laboratoire de Biophysique, Muséum National d’Histoire Naturelle, INSERM U.201 CNRS UA.481, 43 Rue Cuvier, 75005, Paris, France

    C. Helene

  2. Centre de Biophysique Moléculaire, CNRS, 45071, Orléans, Cedex 02, France

    N. T. Thuong

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  1. C. Helene
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Editors and Affiliations

  1. Abteilung Chemie, Max-Planck-Institut für experimentelle Medizin, Hermann-Rein-Straße 3, 3400, Göttingen, Germany

    Fritz Eckstein

  2. Biochemistry Department, University of Dundee, Dundee, DD1 4HN, UK

    David M. J. Lilley

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Helene, C., Thuong, N.T. (1988). Oligo-[α]-Deoxyribonucleotides Covalently Linked to Intercalating Agents. A New Family of Sequence-Specific Nucleic Acid Reagents. In: Eckstein, F., Lilley, D.M.J. (eds) Nucleic Acids and Molecular Biology. Nucleic Acids and Molecular Biology, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-83384-7_6

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  • DOI: https://doi.org/10.1007/978-3-642-83384-7_6

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