Abstract
The susceptibility of the frog embryo to infective transmission of the renal adenocarcinoma was shown following inoculation of R. pipiens embryos with subcellular fractions and filtrates from inclusion body tumors (1). The cytoplasmic fractions injected were rich in the herpes-like virus also seen in the source tumor. Induction of renal carcinomas, identical to spontaneous actively growing primary adenocarcinomas occurred in 66% to over 90% of the hosts. Tumors were found in both pronephric and mesonephric kidneys and were palpable prior to or during metamorphosis. While the success of the injections depended upon the embryonic stage, all recipients from the blastula through hatching stage were inducible. Subsequently, Mulcare (2, 3) has found that similar fractions will transmit renal tumors through the embryos of a closely related species Rana palustris.
This research was supported by Grant CA-07849 from the National Cancer Institute, NIH, Bethesda, Md.
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References
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Tweedell, K.S. (1969). Simulated Transmission of Renal Tumors in Oocytes and Embryos of Rana pipiens . In: Mizell, M. (eds) Biology of Amphibian Tumors. Recent Results in Cancer Research, vol 1969. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-85791-1_20
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