Abstract
The “virus-free” renal adenocarcinoma (Lucké tumor) of the frog has been shown by Mizell et al. (1) to develop herpes-type virus (HTV) when the tumor-bearing frogs are maintained at 7–8°C for several months. Tweedell (2) has shown that kidney tumors can be induced in frog embryos by HTV-positive tumor filtrates and subcellular fractions obtained by the differential centrifugation of tumor homogenates. We have fractionated several of these cold-treated tumors utilizing sucrose gradient zonal centrifugation followed by isopycnic banding of the virus zones for further purification. This report presents the results of these fractionation studies.
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References
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Toplin, I., Brandt, P., Sottong, P. (1969). Density Gradient Centrifugation Studies on the Herpes-Type Virus of the Lucké Tumor. In: Mizell, M. (eds) Biology of Amphibian Tumors. Recent Results in Cancer Research, vol 1969. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-85791-1_29
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DOI: https://doi.org/10.1007/978-3-642-85791-1_29
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