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Sensitive Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 53–84) to Diagnose Hypoparathyroid Conditions: Guidelines for the Establishment of Highly Sensitive Assays

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Calcium Regulating Hormones, Vitamin D Metabolites, and Cyclic AMP Assays and Their Clinical Application

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Abstract

Several sensitive parathyroid hormone (PTH) assays have been published, but differentiation between hypoparathyroid and healthy subjects remains a problem [7, 15, 16, 17, 18, 22, 23]. The main difficulty of the PTH radioimmunoassay (RIA) is the lack of assay sensitivity. This causes hypoparathyroid sera to be mistakenly placed within the normal range or PTH to remain undetectable in normal subjects. We have developed a procedure which is able to differentiate between normal and hypoparathyroid sera [26]. We used the same antiserum as for the “rapid assay” which has been described previously [25]. Guidelines are given for the establishment of highly sensitive assays.

This study was supported by Deutsche Forschungsgemeinschaft (Schm/7–1). Parts of this chapter have been published in Annals of Clinical Biochemistry.

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Authors
  1. D. Zillikens
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Editors and Affiliations

  1. Im Breitspiel 15, D-6900, Heidelberg, Germany

    Heinrich Schmidt-Gayk

  2. Immundiagnostik GmbH, Wilhelmstraße 7, D-6140, Bensheim, Germany

    Franz Paul Armbruster Dipl.Ing. (chem.)

  3. Laboratorium voor Experimentele Geneeskunde en Endocrinologie (LEGENDO), Katholieke Universiteit Leuven, Gasthuisberg, B-3000, Leuven, Germany

    Roger Bouillon

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© 1990 Springer-Verlag Berlin Heidelberg

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Zillikens, D. (1990). Sensitive Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 53–84) to Diagnose Hypoparathyroid Conditions: Guidelines for the Establishment of Highly Sensitive Assays. In: Schmidt-Gayk, H., Armbruster, F.P., Bouillon, R. (eds) Calcium Regulating Hormones, Vitamin D Metabolites, and Cyclic AMP Assays and Their Clinical Application. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-00406-7_6

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  • DOI: https://doi.org/10.1007/978-3-662-00406-7_6

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-00408-1

  • Online ISBN: 978-3-662-00406-7

  • eBook Packages: Springer Book Archive

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