Skip to main content

Enzymatic Aqueous Extraction (EAE)

  • Chapter
  • First Online:
Alternative Solvents for Natural Products Extraction

Abstract

Aqueous enzymatic extraction is employed for fractionation of plant raw material and for extraction of molecules of interest in a safe manner. For many years, the improvement of industrial enzymes lead to new potentialities and new products and implies today an entire rethinking of green extraction and its economic prospects.

This chapter deals with enzymatic aqueous extraction as an alternative method for green extraction. The interests of the use of enzymatic mixtures during green extraction processes of natural molecules are detailed through successful and recent improvements. A focus is done on vegetable products. Advantages and drawbacks of enzymatic-based technologies are described: implementation, availability of enzymes, diversity of activities, development of new enzymatic activities, cost, safety, efficiency, etc.

From lab to industrial scale, examples illustrate the state of the art in enzymatic aqueous extraction.

These technologies are also considered through economical and environmental considerations dealing with actual knowledge. This allows us to envisage future industrial development of enzymatic aqueous extraction processes and to position them as green processes.

Finally, some elements are taken into account in assessing the potential benefits of combining various green technologies to promote synergies with green extraction technologies and improve efficiency, improve the economic balance, and reduce environmental impact.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 54.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Chemat F (2011) Les six principes de l’éco-extraction. In: Eco-extraction du végétal. Procédés innovants et solvants alternatifs. Technique et ingénierie. Dunod Ed, pp 3–24

    Google Scholar 

  2. Johnson L, Lusas E (1983) Comparison of alternative solvents for oils extraction. J Am Oil Chem Soc 60(2):229–242

    CAS  Google Scholar 

  3. Rosenthal A, Pyle DL, Niranjan K (1996) Aqueous and enzymatic processes for edible oil extraction Enzyme. Microb Technol 19(6):402–420

    CAS  Google Scholar 

  4. Yamada T, Sakaguchi K (1982) Comparative studies on chlorella cell walls: induction of protoplast formation. Arch Microbiol 132(1):10–13

    Google Scholar 

  5. Vladimirescu A (2010) Isolation of permeaplasts and spheroplasts from spirulina platensis. Rom Biotechnol Lett 15(3):5361–5368

    Google Scholar 

  6. Blanc G, Duncan G, Agarkova I, Borodovsky M, Gurnon J, Kuo A, Lindquist E, Lucas S, Pangilinan J, Polle J, Salamov A, Terry A, Yamada T, Dunigan D, Grigoriev I, Claverie JM, Van Etten J (2010) The chlorella variabilis NC64A genome reveals adaptation to photosymbiosis, coevolution with viruses, and cryptic sex. Plant Cell 22(9):2943–2955

    CAS  Google Scholar 

  7. Kodner R, Summons R, Knoll A (2009) Phylogenetic investigation of the aliphatic, non-hydrolyzable biopolymer algaenan, with a focus on green algae. Org Geochem 40(8):854–862

    CAS  Google Scholar 

  8. Allard B, Templier J (2001) High molecular weight lipids from the trilaminar outer wall (tls)-containing microalgae Chlorella emersonii, Scenedesmus communis and Tetraedron minimum. Phytochemistry 57(3):459–467

    CAS  Google Scholar 

  9. Domozych D, Ciancia M, Fangel J, Mikkelsen M, Ulvskov P, Willats W (2012) The cell walls of green algae: a journey through evolution and diversity. Front Plant Sci 3(82):1–7

    Google Scholar 

  10. Popper Z, Michel G, Herve C, Domozych D, Willats W, Tuohy M, Kloareg B, Stengel D (2011) Evolution and diversity of plant cell walls: from algae to flowering plants. Annu Rev Plant Biol 62:567–588

    CAS  Google Scholar 

  11. Michel G, Tonon T, Scornet D, Cock J, Kloareg B (2010) The cell wall polysaccharide metabolism of the brown alga Ectocarpus siliculosus. Insights into the evolution of extra-cellular matrix polysaccharides in Eukaryotes. New Phytol 188(1):82–97

    CAS  Google Scholar 

  12. Pasquet V, Cherouvrier JR, Farhat F, Thiery V, Piot JM, Berard JB, Kaas R, Serive B, Patrice T, Cadoret JP, Picot L (2011) Study on the microalgal pigments extraction process: performance of microwave assisted extraction. Process Biochem 46(1):59–67

    CAS  Google Scholar 

  13. Liu C, Lin L (2001) Ultrastructural study and lipid formation of isochrysis sp ccmp1324. Bot Bull Acad Sin 42:207–214

    CAS  Google Scholar 

  14. Zhang Y-HP, Lynd LR (2006) A functionally based model for hydrolysis of cellulose by fungal cellulase. Biotechnol Bioeng 94:888–898

    CAS  Google Scholar 

  15. Howard R, Abotsi E, Jansen van Rensburg EL, Howard S (2003) Lignocellulose biotechnology: issues of bioconversion and enzyme production. Afr J Biotechnol 2(12):602–619

    CAS  Google Scholar 

  16. Shallom D, Shoham Y (2003) Microbial hemicellulases. Curr Opin Microbiol 6(3):219–228

    CAS  Google Scholar 

  17. Silchenko AS, Kusaykin MI, Kurilenko VV, Zakharenko AM, Isakov VV, Zaporozhets TS, Gazha AK, Zvyagintseva TN (2013) Hydrolysis of fucoidan by fucoidanase isolated from the marine bacterium, Formosa algae. Mar Drugs 11(7):2413–2430

    Google Scholar 

  18. Descamps V, Colin S, Lahaye M, Jam M, Richard C, Potin P, Barbeyron T, Yvin JC, Kloareg B (2006) Isolation and culture of a marine bacterium degrading the sulfated fucans from marine brown algae. Marine Biotechnol 8(1):27–39

    CAS  Google Scholar 

  19. Kim EJ, Fathoni A, Jeong GT, Jeong HD, Nam TJ, Kong IS, Kim JK (2013) Microbacterium oxydans, a novel alginate- and laminarin-degrading bacterium for the reutilization of brown-seaweed waste. J Environ Manage 130:153–159

    CAS  Google Scholar 

  20. Suda K, Tanji Y, Hori K, Unno H (1999) Evidence for a novel Chlorella virus- encoded alginate lyase. FEMS Microbiol Lett 180(1):45–53

    CAS  Google Scholar 

  21. Ma S, Tan YL, Yu WG, Han F (2013) Cloning, expression and characterization of a new ι-carrageenase from marine bacterium, Cellulophaga sp. Biotechnol Lett 35(10):1617–1622

    CAS  Google Scholar 

  22. Ma YX, Dong SL, Jiang XL, Li J, Mou HJ (2010) Purification and characterization of carrageenase from marine bacterium mutant strain Pseudoalteromonas Sp. Aj5-13 and its degraded products. J Food Biochem 34:661–678

    CAS  Google Scholar 

  23. Gupta V, Trivedi N, Kumar M, Reddy C, Jha B (2013) Purification and characterization of exo-β-agarase from an endophytic marine bacterium and its catalytic potential in bioconversion of red algal cell wall polysaccharides into galactans. Biomass Bioenerg 49:290–298

    CAS  Google Scholar 

  24. Hatada Y, Ohta Y, Horikoshi K (2006) Hyperproduction and application of alpha-agarase to enzymatic enhancement of antioxidant activity of porphyran. J Agric Food Chem 54(26):9895–9900

    CAS  Google Scholar 

  25. Lahaye M, Brunel M, Bonnin E (1997) Fine chemical structure analysis of oligosaccharides produced by an ulvan-lyase degradation of the water-soluble cell-wall polysaccharides from Ulva sp, (Ulvales, Chlorophyta). Carbohydr Res 304(3–4):325–333

    CAS  Google Scholar 

  26. Sugimoto I, Onimatsu H, Fujie M, Usami S, Yamada T (2004) vAL-1, a novel polysaccharide lyase encoded by chlorovirus CVK2. FEBS Lett 559(1–3):51–56

    CAS  Google Scholar 

  27. Sherba SE, Faith WTJ, Smythe CV, Steigerwalt RB (1972) Soybean fractionation employing protease. Patent US3640725A

    Google Scholar 

  28. Lanzani A, Petrini M, Cozzoli O, Gallavresi P, Carola C, Jacini G (1975) On the use of enzymes for vegetable-oil extraction. A preliminary report. Riv Ital Sostanze Grasse 11:226–229

    Google Scholar 

  29. Fantozzi P, Petruccioli G, Montedoro G (1977) Enzymatic treatment of olive pastes after single pressing extraction. Effect of cultivar, harvesting time, and storage. Rils Ital Sosfanze Grusse 54:381–388

    CAS  Google Scholar 

  30. Fullbrook P (1983) The use of enzymes in the processing of oilseeds. J Am Oil Chem Soc 60(2):476–478

    Google Scholar 

  31. Sosulski K, Sosulski F (1993) Enzyme-aided vs. two-stage processing of canola: technology, product quality and cost evaluation. J Am Oil Chem Soc 70(9):825–829

    CAS  Google Scholar 

  32. Dominguez H, Nunez M, Lema J (1994) Enzymatic pretreatment to enhance oil extraction from fruits and oilseeds: a review. Food Chem 49(3):271–286

    CAS  Google Scholar 

  33. Cerda A, Martinez ME, Soto C, Poirrier P, Perez-Correa JR, Vergara-Salinas JR, Zuniga ME (2013) The enhancement of antioxidant compounds extracted from thymus vulgaris using enzymes and effect of extracting effect. Food Chem 139:138–143

    CAS  Google Scholar 

  34. Zheng H, Hwang I, Kim S, Lee S, Chung S (2010) Optimization of carbohydrate-hydrolyzing enzyme aided polyphenol extraction from unripe apples. J Korean Soc Appl Biol 53(3):342–350

    CAS  Google Scholar 

  35. Nagendra Chari KL, Mannasa D, Srinivas P, Sowbhagya HB (2013) Enzyme-assisted extraction of bioactive compounds from ginger (Zingiber officinale Roscoe). Food Chem 139:509–514

    CAS  Google Scholar 

  36. Olsen H (1988) Aqueous extraction of oil from seeds. In: Asian food conference, Bangkok, Thailande, 24–26 Oct 1988

    Google Scholar 

  37. Sosulski K, Sosulski FW (1990) Enzyme pre-treatment to enhance oil extractability in canola. In: Shahidi F (ed) Canola and rapeseed: production, chemistry, nutrition and processing technology. Van Nostrand Reinhold Publishers, New York, pp 277–289

    Google Scholar 

  38. Deng Y, Pyle D, Niranjan K (1992) Studies of aqueous enzymatic extraction of oil from rapeseed. Agric Eng Rural Dev 1 Conf Proc 1

    Google Scholar 

  39. Kim IH, Yoon SH (1990) Effect of extraction solvents on oxidative stability of crude soybean oil. J Am Oil Chem Soc 67(3):165–167

    CAS  Google Scholar 

  40. Bargale PC, Sosulski K, Sosulski FW (2000) Enzymatic hydrolysis of soybean for solvent and mechanical oil extraction. J Food Process Eng 23(4):321–327

    Google Scholar 

  41. Bocevska M, Karlovic D, Turkulov J, Pericin D (1993) Quality of corn germ oil obtained by aqueous enzymatic extraction. J Am Oil Chem Soc 70(12):1273–1277

    CAS  Google Scholar 

  42. Ranalli A, Martinelli N (1994) Extraction of the oil from the olive pastes by biological and not conventional industrial technics. Ind Alimentari 33(331):1073–1083

    CAS  Google Scholar 

  43. Ranalli A, Martinelli N (1995) Integral centrifuges for olive oil extraction, at the third millennium threshold. Transformation yields. Grasas Y Aceites 46(4–5):255–263

    CAS  Google Scholar 

  44. McGlone O, Lopez-Munguia C, Cater J (1986) Coconut oil extraction by a new enzymatic process. J Food Sci 51(3):695–697

    CAS  Google Scholar 

  45. Christensen F (1989) Enzyme technology versus engineering technology in the food industry. Biotechnol Appl Biochem 11:249–265

    CAS  Google Scholar 

  46. Buenrostro M, Lopez-Munguia CA (1986) Enzymatic extraction of avocado oil. Biotechnol Lett 8(7):505–506

    CAS  Google Scholar 

  47. Freitas SP, Lago RCA, Jablonka FH, Hartman L (1993) Aqueous enzymatic extraction of avocado oil from fresh pulp. Revue Francaise des Corps Gras 40:365–371

    CAS  Google Scholar 

  48. Dominguez H, Sineiro J, Nunez M, Lema J (1995) Enzymatic treatment of sunflower kernels before oil extraction. Food Res Int 28(6):537–545

    CAS  Google Scholar 

  49. Cheah SC, Augustin MA, Ooi L (1990) Enzymatic extraction of palm oil. Palm Oil Res Malaysia Bull 20:30–36

    Google Scholar 

  50. Mosier N, Wyman C, Dale B, Elander R, Lee Y, Holtzapple M, LaDisch M (2005) Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresour Technol 96(6):673–686

    CAS  Google Scholar 

  51. Van Eylen D, Indrawati M, Hendrickx M, Van Loey A (2006) Temperature and pressure stability of mustard seed (Sinapis alba L.) myrosinase. Food Chem 97:263–267

    Google Scholar 

  52. Zhang Z, Ober JA, Kliebenstein DJ (2006) The gene controlling the quantitative trait locus EPITHIOSPECIFIER MODIFIER1 alters glucosinolate hydrolysis and insect resistance in Arabidopsis. Plant Cell 18(6):1524–1536

    CAS  Google Scholar 

  53. Carre B (2000) Effets de la taille des particules alimentaires sur les processus digestifs chez les oiseaux d’élevage. INRA Prod Anim 13(2):131–136

    Google Scholar 

  54. Rosenthal A, Pyle D, Niranjan K (1998) Mechanisms in the simultaneous aqueous extraction of oil and protein from soybean. Food Bioprod Process 76:224–230

    CAS  Google Scholar 

  55. Dickey L, Kurantz M, Parris N (2008) Oil separation from wet-milled corn germ dispersions by aqueous oil extraction and aqueous enzymatic oil extraction. Ind Crops Prod 27(3):303–307

    CAS  Google Scholar 

  56. Evon P, Vandenbossche V, Pontalier PY, Rigal L (2007) Direct extraction of oil from sunflower seeds by twin-screw extruder according to an aqueous extraction process: feasibility study and influence of operating conditions. Ind Crops Prod 2:351–359

    Google Scholar 

  57. Weil J, Sarikaya A, Rau SL, Goetz J, Ladisch C, Brewer M, Hendrickson R, Ladisch M (1997) Pretreatment of yellow poplar sawdust by pressure cooking in water. Appl Biochem Biotechnol 68(1–2):21–40

    CAS  Google Scholar 

  58. Shankar D, Agrawal Y, Sarkar B, Singh B (1997) Enzymatic hydrolysis in conjunction with conventional pretreatments to soybean for enhanced oil availability and recovery. J Am Oil Chem Soc 74(12):1543–1547

    CAS  Google Scholar 

  59. Weil J, Brewer M, Hendrickson R, Sarikaya A, Ladisch M (1998) Continuous pH monitoring during pretreatment of yellow poplar wood sawdust by pressure cooking in water. Appl Biochem Biotechnol 70(2):99–111

    Google Scholar 

  60. Lee Y, Wu Z, Torget R (2000) Modeling of countercurrent shrinking-bed reactor in dilute-acid total-hydrolysis of lignocellulosic biomass. Bioresour Technol 71(1):29–39

    CAS  Google Scholar 

  61. Chang V, Holtzapple M (2000) Fundamental factors affecting biomass enzymatic reactivity. Appl Biochem Biotechnol 84(6):5–37

    Google Scholar 

  62. Chang V, Nagwani M, Holtzapple M (1998) Lime pretreatment of crop residues bagasse and wheat straw. Appl Biochem Biotechnol 74(3):135–159

    CAS  Google Scholar 

  63. Chang V, Nagwani M, Kim C, Holtzapple M (2001) Oxidative lime pretreatment of high-lignin biomass – poplar wood and newspaper. Appl Biochem Biotechnol 94(1):1–28

    CAS  Google Scholar 

  64. Jiang L, Hua D, Wang Z, Shiying X (2010) Aqueous enzymatic extraction of peanut oil and proteins hydrolysates. Food Bioprod Process 88(C2–3):233–238

    CAS  Google Scholar 

  65. Mathlouthi N, Saulnier L, Quemener B, Larbier M (2002) Xylanase, b-glucanase, and other side enzymatic activities have greater effects on the viscosity of several feedstuffs than xylanase and b-glucanase used alone or in combination. J Agric Food Chem 50(18):5121–5127

    CAS  Google Scholar 

  66. Ramadan MF, Morsel J-T (2009) Oil extractability from enzymatically-treated goldenberry (Physalis peruviana L.) pomace: range of operational variables. Int J Food Sci Technol 44:435–444

    CAS  Google Scholar 

  67. Kurmudle N, Kagliwal LD, Bankar SB, Singhal RS (2013) Enzyme-assisted extraction for enhanced yields of turmeric oleoresin and its constituents. Food Biosci 3:36–41

    Google Scholar 

  68. Rosenthal A, Pyle DL, Niranjan K, Gilmour S, Trinca L (2001) Combined effect of operational variables and enzyme activity on aqueous enzymatic extraction of oil and protein from soybean. Enzyme Microb Technol 28(6):499–509

    CAS  Google Scholar 

  69. Tabtabaei S, Diosady LL (2013) Aqueous and enzymatic extraction processes for the production of food-grade proteins and industrial oil from dehulled yellow mustard flour. Food Res Int 52(2):547–556

    CAS  Google Scholar 

  70. Hagenmaier FD (1974) Aqueous processing of full-fat sunflower seeds: yields of oil and protein. J Am Oil Chem Soc 51(10):470–471

    CAS  Google Scholar 

  71. Badr FH, Sitohy MZ (1992) Optimizing conditions for enzymatic extraction of sunflower oil. Grasas y Aceites 43(5):281–283

    CAS  Google Scholar 

  72. Raghavendra SN, Raghavarao KSMS (2011) Aqueous extraction and enzymatic destabilization of coconut milk emulsions. J Am Oil Chem Soc 88:481–487

    CAS  Google Scholar 

  73. Najafian L, Ghodsvali A, Haddad Khodaparast M, Diosady L (2009) Aqueous extraction of virgin olive oil using industrial enzymes. Food Res Int 42(1):171–175

    CAS  Google Scholar 

  74. Tano-Debrah K, Ohta Y (1997) Aqueous extraction of coconut oil by an enzyme-assisted process. J Sci Food Agric 74(4):497–502

    CAS  Google Scholar 

  75. Towa LT, Kapchie VN, Hauck C, Murphy PA (2010) Enzyme assisted aqueous-extraction of oil from isolated oleosomes of soybean flour. J Am Oil Chem Soc 87:347–354

    CAS  Google Scholar 

  76. Latif S, Anwar F (2011) Aqueous enzymatic sesame oil and protein extraction. Food Chem 125:679–684

    CAS  Google Scholar 

  77. Zhang S, Wang Z, Xu SY (2007) Optimization of the aqueous enzymatic extraction of rapeseed oil and protein hydrolysates. J Am Oil Chem 84:97–105

    CAS  Google Scholar 

  78. Latif S, Anwar F (2009) Effect of aqueous enzymatic processes on sunflower oil quality. J Am Oil Chem Soc 86(4):393–400

    CAS  Google Scholar 

  79. Soto C, Chamy R, Zuniga ME (2007) Enzymatic hydrolysis and pressing conditions effect on borage oil extraction by cold pressing. Food Chem 102:834–840

    CAS  Google Scholar 

  80. Jung S, Maurer D, Johnson LA (2009) Factors affecting emulsion stability and quality of oil recovered from enzyme-assisted aqueous extraction of soybeans. Bioresour Technol 100(21):5340–5347

    CAS  Google Scholar 

  81. Moure A, Sineiro J, Dominguez H, Parajo JC (2006) Functionnality of oilseed protein products: a review. Food Res Int 39:945–963

    CAS  Google Scholar 

  82. Bagnasco L, Pappalardo VM, Meregaglia A, Kaewmanee T, Ubiali D, Speranza G, Cosulich ME (2013) Use of food grade proteases to recover protein-peptide mixtures from rice middlings. Food Res Int 50:420–427

    CAS  Google Scholar 

  83. Aliakbarian B, De Faveri D, Converti A, Perego P (2008) Optimization of olive oil extraction by means of enzyme processing aids using response surface methodology. Biochem Eng J 42(1):34–40

    CAS  Google Scholar 

  84. Fan Y, Wu X, Zhang M, Zhao T, Zhou Y, Han L, Yang L (2011) Physical characteristics and antioxidant effect of polysaccharides extracted by boiling water and enzymolysis from Grifola frondosa. Int J Biol Macromol 48(5):798–803

    CAS  Google Scholar 

  85. Fu YJ, Liu W, Zu YG, Tong MH, Li SM, Yan MM, Efferth T, Luo H (2008) Enzyme assisted extraction of luteolin and apigenin from pigeonpea [Cajanus cajan (L.) Millsp.] leaves. Food Chem 111(2):508–512

    CAS  Google Scholar 

  86. Chabrand RM, Glatz CE (2009) Destabilization of the emulsion formed during the enzyme-assisted aqueous extraction of oil from soybean flour. Enzym Microb 45:28–35

    CAS  Google Scholar 

  87. Li Y, Sui X, Qi B, Zhang Y, Feng H, Zhang Y, Jiang L, Wang T (2014) Optimization of ethanol-ultrasound-assisted destabilization of a cream recovered from enzymatic extraction of soybean oil. J Am Oil Chem Soc 91(1):159–168

    CAS  Google Scholar 

  88. Chen S, Xing XH, Huang JJ, Xu MS (2011) Enzyme-assisted extraction of flavonoids from Ginkgo biloba leaves: improvement effect of flavonol transglycosylation catalyzed by Penicillium decumbens cellulase. Enzyme Microb Technol 48(1):100–105

    CAS  Google Scholar 

  89. Xu MS, Chen S, Wang WQ, Liu SQ (2013) Employing bifunctional enzymes for enhanced extraction of bioactives from plants: flavonoids as an example. J Agric Food Chem 61(33):7941–7948

    CAS  Google Scholar 

  90. Li J, Zu YG, Luo M, Gu CB, Zhao CJ, Efferth T, Fu YJ (2013) Aqueous enzymatic process assisted by microwave extraction of oil from yellow horn (Xanthoceras sorbifolia Bunge.) seed kernels and its quality evaluation. Food Chem 138(4):2152–2158

    CAS  Google Scholar 

  91. Jiao J, Li ZG, Gai QY, Li XJ, Wei FY, Fu YJ, Ma W (2014) Microwave-assisted aqueous enzymatic extraction of oil from pumpkin seeds and evaluation of its physicochemical properties, fatty acid compositions and antioxidant activities. Food Chem 147:17–24

    CAS  Google Scholar 

  92. Gai QY, Jiao J, Wei FY, Luo M, Wang W, Zu YG, Fu YJ (2013) Enzyme-assisted aqueous extraction of oil from Forsythia suspense seed and its physicochemical property and antioxidant activity. Ind Crops Prod 51:274–278

    CAS  Google Scholar 

  93. Liu S, Jiang L, Li Y (2011) Research of aqueous enzymatic extraction of watermelon seed oil of ultrasonic pretreatment assisted. Proced Eng 15:4949–4955

    CAS  Google Scholar 

  94. Konwarh R, Pramanik S, Kalita D, Mahanta CL, Karak N (2012) Ultrasonication, a complementary “green chemistry” tool to biocatalysis: a laboratory-scale study of lycopene extraction. Ultrason Sonochem 19(2):292–299

    CAS  Google Scholar 

  95. Chen R, Li S, Liu C, Yang S, Li X (2012) Ultrasound complex enzymes assisted extraction and biochemical activities of polysaccharides from Epimedium leaves. Process Biochem 47(12):2040–2050

    Google Scholar 

  96. Da Porto C, Decorti D, Natolino A (2013) Effect of commercial enzymatic preparation with pectolytic activities on conventional extraction and ultrasound-assisted extraction of oil from grape seed (Vitis vinifera L.). Int J Food Sci Technol 48:2127–2132

    Google Scholar 

  97. Adulkar TV, Rathod VK (2014) Ultrasound assisted enzymatic pre-treatment of high fat content dairy wastewater. Ultrason Sonochem 21(3):1083–1089

    CAS  Google Scholar 

  98. Hosni K, Hassen I, Chaâbane H, Jemli M, Dallali S, Sebei H, Casabianca H (2013) Enzyme-assisted extraction of essential oils from thyme (Thymus capitatus L.) and rosemary (Rosmarinus officinalis L.): impact on yield, chemical composition and antimicrobial activity. Ind Crops Prod 47:291–299

    CAS  Google Scholar 

  99. Kapturowska AU, Stolarzewicz IA, Krzyczkowska J, Białecka-Florjańczyk E (2012) Studies on the lipolytic activity of sonicated enzymes from Yarrowia lipolytica. Ultrason Sonochem 19(1):186–191

    CAS  Google Scholar 

  100. Kapchie VN, Wei D, Hauck C, Murphy PA (2008) Enzyme-assisted aqueous extraction of oleosomes from soybeans (Glycine max). J Agric Food Chem 56(5):1766–1771

    CAS  Google Scholar 

  101. Sharma A, Khare SK, Gupta MN (2002) Enzyme-assisted aqueous extraction of peanut oil. J Am Oil Chem Soc 79(3):215–218

    CAS  Google Scholar 

  102. Schmid A, Dordick JS, Hauer B, Kiener A, Wubbolts M, Witholt B (2001) Industrial biocatalysis today and tomorrow. Nature 409(6817):258–268

    CAS  Google Scholar 

  103. Schmid A, Hollmann F, Park JB, Bühler B (2002) The use of enzymes in the chemical industry in Europe. Curr Opin Biotechnol 13(4):359–366

    CAS  Google Scholar 

  104. Rolle RS (1998) Review: enzyme applications for agro-processing in developing countries: an inventory of current and potential applications. World J Microbiol Biotechnol 14:611–619

    CAS  Google Scholar 

  105. Ellis RJ (1979) Reviews the most abundant protein in the world. Trends Biochem Sci 4:241–244

    CAS  Google Scholar 

  106. Kapel R, Chabeau A, Lesage J, Riviere G, Ravallecple R, Lecouturier D, Wartelle M, Guillochon D, Dhulster P (2006) Production, in continuous enzymatic membrane reactor, of an anti-hypertensive hydrolysate from an industrial alfalfa white protein concentrate exhibiting ACE inhibitory and opioid activities. Food Chem 98(1):120–126

    CAS  Google Scholar 

  107. Boschetti E, Righetti PG (2012) Breakfast at Tiffany’s? Only with a low-abundance proteomic signature! Electrophoresis 33(15):2228–2239

    CAS  Google Scholar 

  108. Andersson I, Backlund A (2008) Structure and function of Rubisco. Plant Physiol Biochem 46(3):275–291

    CAS  Google Scholar 

  109. Puri M, Sharma D, Barrow CJ (2012) Enzyme-assisted extraction of bioactives from plants. Trends Biotechnol 30(1):37–44

    CAS  Google Scholar 

  110. Alkorta I, Garb C (1998) Industrial applications of pectic enzymes: a review. Process Biochem 33(I):21–28

    CAS  Google Scholar 

  111. De Maria L, Vind J, Oxenbøll KM, Svendsen A, Patkar S (2007) Phospholipases and their industrial applications. Appl Microbiol Biotechnol 74(2):290–300

    CAS  Google Scholar 

  112. Houde A, Kademi A, Leblanc D (2004) Lipases and their industrial applications: an overview. Appl Biochem Biotechnol 118(1–3):155–170

    CAS  Google Scholar 

  113. Zuniga M, Soto C, Mora A, Chamy R, Lema J (2003) Enzymic pre-treatment of Guevina avellana mol oil extraction by pressing. Process Biochem 39:51–57

    CAS  Google Scholar 

  114. Dehghan-Shoar Z, Hardacre AK, Meerdink G, Brennan CS (2011) Lycopene extraction from extruded products containing tomato skin. Int J Food Sci Technol 46(2):365–371

    CAS  Google Scholar 

  115. Kamm B, Kamm M (2004) Principles of biorefineries. Appl Microbiol Biotechnol 64(2):137–145

    CAS  Google Scholar 

  116. Klein-Marcuschamer D, Oleskowicz-Popiel P, Simmons BA, Blanch HW (2012) The challenge of enzyme cost in the production of lignocellulosic biofuels. Biotechnol Bioeng 109(4):1083–1087

    CAS  Google Scholar 

  117. Veit T (2004) Biocatalysis for the production of cosmetic ingredients. Eng Life Sci 4(6):508–511

    CAS  Google Scholar 

  118. Bornscheuer UT (2003) Immobilizing enzymes: how to create more suitable biocatalysts. Angewandte Chemie (International ed. in English) 42(29):3336–3337

    Google Scholar 

  119. Sheldon R (2007) Enzyme immobilization: the quest for optimum performance. Adv Synth Catal 349(8–9):1289–1307

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Lionel Muniglia .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Muniglia, L., Claisse, N., Baudelet, PH., Ricochon, G. (2014). Enzymatic Aqueous Extraction (EAE). In: Chemat, F., Vian, M. (eds) Alternative Solvents for Natural Products Extraction. Green Chemistry and Sustainable Technology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-43628-8_8

Download citation

Publish with us

Policies and ethics