Zusammenfassung
Es ist immer eine gute Idee, Antikörper gegen ein gereinigtes Protein herzustellen. Antikörper ermöglichen Affinitätssäulen, Nachweistests, histologische Untersuchungen an Gewebeschnitten und das Screenen von Expressionsbanken. Sie informieren über die Lage eines Transmembranproteins in der Membran und über die Zusammensetzung und Stöchiometrie oligomerer Proteine. Lesen Sie, wie man Antikörper herstellt, bearbeitet, verarbeitet, pflegt und anwendet.
Zum Guten noch was Besseres: In bestimmten Fällen sind Aptamere den Antikörpern überlegen.
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Rehm, H., Letzel, T. (2016). Antikörper und Aptamere. In: Der Experimentator: Proteinbiochemie/Proteomics. Experimentator. Springer Spektrum, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-48851-5_6
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