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Proteomic profiling of cellular stresses in Bacillus subtilis reveals cellular networks and assists in elucidating antibiotic mechanisms of action

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Systems Biological Approaches in Infectious Diseases

Part of the book series: Progress in Drug Research ((PDR,volume 64))

Abstract

Proteomic profiling provides a global view of the protein composition of the cell. In contrast to the static nature of the genome sequence, which provides the blueprint for all protein-based cellular building blocks, the proteome is highly dynamic. The protein composition is constantly adjusting to facilitate survival, growth, and reproduction in an ever-changing environment. In a quest to understand the regulation of cellular networks in bacteria and the role of individual proteins in the adaptation process, the proteomic response to stress and starvation was analyzed in wild-type and mutant strains. The knowledge derived from these proteomic studies was applied to investigating the bacterial response to antibiotics. It was found that proteomics presents a powerful tool for hypothesis generation regarding antibiotic mechanism of action.

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Author information

Authors and Affiliations

  1. Pfizer Global Research and Development, Pfizer Inc., Ann Arbor, Michigan, USA

    Julia E. Bandow

  2. Institute for Microbiology, Ernst-Moritz-Arndt Universität Greifswald, Greifswald, Germany

    Michael Hecker

Authors
  1. Julia E. Bandow
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  2. Michael Hecker
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Editor information

Editors and Affiliations

  1. Tuberculosis Research Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, Rockville, MD, 20852, USA

    Helena I. Boshoff  & Clifton E. Barry III  & 

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© 2007 Birkhäuser Verlag

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Bandow, J.E., Hecker, M. (2007). Proteomic profiling of cellular stresses in Bacillus subtilis reveals cellular networks and assists in elucidating antibiotic mechanisms of action. In: Boshoff, H.I., Barry, C.E. (eds) Systems Biological Approaches in Infectious Diseases. Progress in Drug Research, vol 64. Birkhäuser Basel. https://doi.org/10.1007/978-3-7643-7567-6_4

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