Summary
A new method for the immobilization of lumbrokinase (LK) on polymer was developed. The method is based on the photochemical reaction of the azidophenyl group, which produces a highly reactive phenylnitrene upon ultraviolet (UV) light irradiation. First, an aminated polymer, partially derivatized with a photoreactive phenyl azido group in its side chains, was coated onto a polyurethane (PU) surface. Subsequently, the surface was exposed to UV light. To induce the carboxyl group on the UV-light-treated surface, poly(methyl vinyl ether-co-maleic acid) (MAcMEC) was reacted. LK was immobilized on the PU surface using l-ethyl-3-(3-dimethylaminopropyl)carbo-diimide (EDC) in aqueous solvent. Electron spectroscopy for chemical analysis (ESCA) and water contact angle measurement before and after sequential surface reactions revealed that LK was immobilized successfully. The LK immobilized on the PU surface had fibrinolytic activity. In conclusion, LK immobilization via this photochemical reaction will be useful in improving the blood compatibility of blood-contacting polymer.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Ryu GH, Han DK, Park S, Kim MR, Min BG (1995) Surface characteristics and properties of lumbrokinaseimmobilized PU. J Biomed Mater Res 29:403–409
Sugawara T, Matsuda T (1994) Novel surface graft copolymerization method with micro-order regional precision. Macromolecules 27:7809–7814
Sano S, Kato K, Ikada Y (1993) Introduction of functional groups onto the surface of polyethylene for protein immobilization. Biomaterials 14(11):817–822
Cai SX, Wybourne MN, Keana JFW (1994) N-hydroxysuccinimide ester functionalized perfluorophenyl azides as novel photoreactive heterobifunctional cross-linking reagents. The covalent immobilization of biomolecules to polymer surfaces. Bioconjug Chem 5(2):151–157
Massia SP, Hubble JA (1990) Covalent surface immobilization of Arg-Gly-Asp and Tyr-IIe-Gly-Ser-Argcontaining peptides to obtain well-defined cell adhesion substrates. Anal Chem 187:292–301
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer-Verlag Tokyo
About this paper
Cite this paper
Kim, H.J., Nakayama, Y., Kim, J., Matsuda, T., Min, B.G. (1998). Surface Fixation of Lumbrokinase via Photochemical Reaction of Azidophenyl Group. In: Akutsu, T., Koyanagi, H. (eds) Heart Replacement. Springer, Tokyo. https://doi.org/10.1007/978-4-431-65921-1_74
Download citation
DOI: https://doi.org/10.1007/978-4-431-65921-1_74
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-65923-5
Online ISBN: 978-4-431-65921-1
eBook Packages: Springer Book Archive