Monitoring of Cell Activity: Online Oxygen Uptake Rates in Pulsed Aerated Cell Culture

Bock, A.; Genzel, Y.; Reichl, U.

doi:10.1007/978-90-481-3419-9_68

A. Bock²,
Y. Genzel³ &
U. Reichl^2,4

Part of the book series: ESACT Proceedings ((ESACT,volume 4))

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Abstract

Oxygen uptake rates of adherently growing mammalian Madin Darby canine kidney cells were determined online based on the decrease of dissolved oxygen in a pulsed aerated 5 L stirred tank bioreactor. From these oxygen uptake rates and the average specific oxygen demand of the cells, viable cell numbers were estimated. The calculated cell numbers correlated well with experimentally determined viable cell numbers during exponential growth. However, differences between estimated and experimental cell numbers were identified during density dependent growth inhibition before confluence and during virus replication. Lower specific oxygen uptake rate of cell cycle arrested cells resulted in inhomogeneous cell populations. During virus replication the estimated cell numbers correlated more with the subpopulation of uninfected cells then with the experimentally determined ‘viable’ cell numbers using trypan blue dye.

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References

Genzel, Y. et al. (2006) Serum-free influenza virus production avoiding washing steps and medium exchange in large-scale microcarrier culture. Vaccine 24(16), 3261–3272.
Article PubMed CAS Google Scholar
Ramirez, O.T. and Mutharasan, R. (1990). Cell cycle- and growth phase-dependent variations in size distribution, antibody productivity, and oxygen demand in hybridoma cultures. Biotechnol. Bioeng. 36, 839–848.
Article PubMed CAS Google Scholar
Schuegerl, K. (1991) Biotechnology Vol. 4: Measuring, Modelling and Control. p 36. Rehm, H.-J. and Reed, G. (eds.), VCH Verlagsgesellschaft.
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Authors and Affiliations

Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106, Magdeburg, Germany
A. Bock & U. Reichl
Bioprocess Engineering group, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106, Magdeburg, Germany
Y. Genzel
Otto-von-Guericke-University, Magdeburg, Germany
U. Reichl

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A. Bock
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Y. Genzel
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U. Reichl
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Correspondence to A. Bock .

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Technische Fak., AG Zellkulturtechnik, Universität Bielefeld, Bielefeld, Germany
Thomas Noll

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Bock, A., Genzel, Y., Reichl, U. (2010). Monitoring of Cell Activity: Online Oxygen Uptake Rates in Pulsed Aerated Cell Culture. In: Noll, T. (eds) Cells and Culture. ESACT Proceedings, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3419-9_68

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DOI: https://doi.org/10.1007/978-90-481-3419-9_68
Published: 02 June 2010
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-3418-2
Online ISBN: 978-90-481-3419-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)

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