Abstract
Widefield Surface Plasmon Resonance (WSPR) microscope was used to investigate cell surface interactions under two different culture conditions: bone cells cultured on SPR substrate with transforming growth factor β3 (TGF-β3) and without as control. Trypsinisation was carried out in order to investigate its effect on cell detachment, in the presence of TGF-β3, HCl or BSA/HCl solutions. Trypsin was therefore added to four groups of bone cells with addition of TGF-β3, HCl, HCl/BSA solutions and one additional flask as control. These results further confirmed that application of TGF-β3, HCl and HCl/BSA decreased the degree of cell attachment on surface of culture flasks. HCl and BSA/HCl were tested as they are the carriers and solvents for TGF-β3. Cell detachment in control was about 43% after 6 min, which is slow. Bone cells in the presence of BSA/HCl started detaching from the surface about 4–5 min and cell detachment was about 63% after 6 min which was faster as compared to the control. Bone cells in the presence of HCl alone started detaching from the surface about 2 min (after applying trypsin) and cell detachment was about 69% after 6 min which was faster compared to the BSA/HCl and control. Trypsinisation experiments for bone cells cultured with TGF-β3 (50 ng/ml) showed that cells started to detach from the surface about 1 min after application of trypsin and cell detachment was about 85% after 4 min which was faster as compared to the control, HCl and BSA/HCl.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Critchlow, M.A., Blands, Y.S., Ashhurst, D.E.: The effect of exogenous transforming growth factor beta-2 on healing fractures in the rabbit. Bone 6, 521 (1995)
Freshney, R.I.: Culture of Animal Cells, A manual of Basic Technique, 3rd edn. Wiley-Liss, New York (1994)
Erlebacher, A., Derynck, R.: Increased Expression of TGF-Beta2 in osteoblast results in an osteoprosis-like phenotype. J. Cell Biol. 132, 195–210 (1996)
Gordon, K.J., Blobe, B.C.: Role of transforming growth factor-β superfamily signaling pathways in human disease. Biochimica et Biophysica Acta (BBA). Molecul. Basis Disease 1782(4), 197–228 (2008)
Border, W.A., Nobel, A.A.: Transforming growth factor β in tissue fibrosis. New Engl. J. Med. 331, 1286–1292 (1994)
Itoh, S., Itoh, F., Goumans, M.J., Ten, D.P.: Signaling of transforming growth factor-β family members through Smad proteins. FEBS J. 267(24), 6954–6967 (2000)
Lopez-Casillas, F., Payne, H.M., Andres, J.L., Messague, J.: Betaglycan can act as a dual modulator of TGF-beta access to signaling receptors: mapping of ligand binding and GAG attachment sites. J. Cell Biol. 124(4), 557–567 (1994)
Khan, S.N.: Bone growth factors. Orthop. Clin. 31, 375 (2000)
Abdul Jamid, M.M., Denyer, M.C.T., Youseffi, M., Britland, S.T., Liu, S., See, C.W., Somekh, M.G., Marlafeka, S.: Monitoring immunostaining process of proteins by using a widefield surface plasmon microscope. J. Anat. 206, 515 (2005)
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer Science+Business Media B.V.
About this chapter
Cite this chapter
Sefat, F., Youseffi, M., Denyer, M. (2010). Biological Application of Widefield Surface Plasmon Resonance Microscope to Study Cell/Surface Interactions and the Effect of TGF-β3, HCL and BSA/HCL on Cell Detachment Assay of Bone Cells Monolayer. In: Ao, SI., Gelman, L. (eds) Electronic Engineering and Computing Technology. Lecture Notes in Electrical Engineering, vol 60. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-8776-8_49
Download citation
DOI: https://doi.org/10.1007/978-90-481-8776-8_49
Published:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-8775-1
Online ISBN: 978-90-481-8776-8
eBook Packages: EngineeringEngineering (R0)