Abstract
Cells reflecting in vivo properties are of high interest for modern life sciences. Existing cellular models are either lacking biological relevance or are not available in sufficient amounts. To generate cells in sufficient numbers, cell expansion in vitro is an attractive strategy. We developed a transcriptionally regulated system for the control of immortalizing genes. This system is based on the tet-system. The robustness of these tet-controlled expression circuits allowed us to establish endothelial cell lines, with strictly controlled expansion, from various cell types of murine and human origin. These novel cell systems retain relevant cellular properties rendering them attractive tools for modern drug development and in the long run for cell therapeutic approaches.
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May, T., Butueva, M., Bantner, S., Weich, H., Hauser, H., Wirth, D. (2012). Human Endothelial Cell Lines with In Vivo Physiology. In: Jenkins, N., Barron, N., Alves, P. (eds) Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009. ESACT Proceedings, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0884-6_34
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DOI: https://doi.org/10.1007/978-94-007-0884-6_34
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