Abstract
A pulsed, tapered cylindrical plasma jet, several centimeter long and <2 mm in diameter, has been generated by a concentric tubular device for root canal disinfection. This plasma dental probe is typically powered with ∼100 ns, 1 kHz, multi-kilovolt electric pulses and filled with 5 SLPM (standard liter per minute) He/(1%)O2 flow. We report here an in vitro study of the antimicrobial effect of the room temperature plasma jet against monolayer Enterococcus faecalis biofilms on bovine dentins. Resultant colony-forming unit counts were associated with changes in bacterial cell morphology observed using scanning electron microscopy (SEM) following the treatment and control. Treatment of dentin discs cultivated with E. faecalis monolayer biofilms with the plasma (average power ≈ 1 W) for 5 min resulted in 92.4% kill (P < 0.0001). Severe disruption of the cell membranes was observed for the plasma treatment group, while the morphology of the cells remained intact for the negative control group. In addition, a pilot ex vivo test was conducted to examine the bactericidal effect of the plasma against saliva-derived biofilms cultivated in human root canals. Conspicuous biofilm disruption and cleared dentinal surfaces were observed in the canal after the plasma treatment for 5 min. We conclude that this non-thermal pulsed plasma-based technology is a potential alternative or supplement to existing protocols for root canal disinfection.
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References
Lee K, Paek KH, Ju WT, Lee Y (2006) Sterilization of bacteria, yeast, and bacterial endospores by atmospheric-pressure cold plasma using helium and oxygen. J Microbiol 44:269–275
Deng XT, Shi JJ, Chen HL, Kong MG (2007) Protein destruction by atmospheric pressure glow discharges. Appl Phys Lett 90:13903
Brown IG, Bjornstad KA, Blakely EA, Galvin JE, Monteiro OR, Sangyuenyongpipat S (2003) Growth of large patterned arrays of neurons using plasma methods. Plasma Phys Control Fusion 45:547–554
Fridman G, Peddinghaus M, Ayan H, Fridman A, Balasubramanian M, Gutsol A, Brooks A, Friedman G (2006) Blood coagulation and living tissue sterilization by floating-electrode dielectric barrier discharge in air. Plasma Chem Plasma Process 26:425–442
Weltmann KD, Kindel E, von Woedtke T, Hahnel M, Stieber M, Brandenburg R (2010) Atmospheric-pressure plasma sources: prospective tools for plasma medicine. Pure Appl Chem 82:1223–1237
Sladek REJ, Stoffels E, Walraven R, Tielbeek PJA, Koolhoven RA (2004) Plasma treatment of dental cavities: a feasibility study. IEEE Trans Plasma Sci 32:1540–1543
Jiang CQ, Chen MT, Gorur A, Schaudinn C, Jaramillo DE, Costerton JW, Sedghizadeh PP, Vernier PT, Gundersen MA (2009) Nanosecond pulsed plasma dental probe. Plasma Processes Polym 6:479–483
Lee HW, Nam SH, Mohamed AAH, Kim GC, Lee JK (2010) Atmospheric pressure plasma jet composed of three electrodes: application to tooth bleaching. Plasma Processes Polym 7:274–280
Jiang CQ, Chen MT, Schaudinn C, Gorur A, Vernier PT, Costerton JW, Jaramillo DE, Sedghizadeh PP, Gundersen MA (2009) Pulsed atmospheric-pressure cold plasma for endodontic disinfection. IEEE Trans Plasma Sci 37:1190–1195
Heling I, Chandler NP (1998) Antimicrobial effect of irrigant combinations within dentinal tubules. Int Endod J 31:8–14
Menezes MM, Valera MC, Jorge AO, Koga-Ito CY, Camargo CH, Mancini MN (2004) In vitro evaluation of the effectiveness of irrigants and intracanal medicaments on microorganisms within root canals. Int Endod J 37:311–319
Colak M, Evcil S, Bayindir YZ, Yigit N (2005) The effectiveness of three instrumentation techniques on the elimination of enterococcus faecalis from a root canal: an in vitro study. J Contemp Dent Pract 6:94–106
Nair PN, Sjogren U, Krey G, Kahnberg KE, Sundqvist G (1990) Intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy-resistant periapical lesions: a long-term light and electron microscopic follow-up study. J Endod 16:580–588
Sjogren U, Figdor D, Persson S, Sundqvist G (1997) Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J 30:297–306
Costerton JW, Stewart PS, Greenberg EP (1999) Bacterial biofilms: a common cause of persistent infections. Science 284:1318–1322
Estrela C, Sydney GB, Figueiredo JA, Estrela CR (2009) Antibacterial efficacy of intracanal medicaments on bacterial biofilm: a critical review. J Appl Oral Sci 17:1–7
Gomes BP, Souza SF, Ferraz CC, Teixeira FB, Zaia AA, Valdrighi L, Souza-Filho FJ (2003) Effectiveness of 2% chlorhexidine gel and calcium hydroxide against enterococcus faecalis in bovine root dentine in vitro. Int Endod J 36:267–275
Vianna ME, Gomes BP, Berber VB, Zaia AA, Ferraz CC, de Souza-Filho FJ (2004) In vitro evaluation of the antimicrobial activity of chlorhexidine and sodium hypochlorite. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 97:79–84
Jiang C, Puech V, Magne L, Jeanney P (2009) Absolute ozone measurements for a low-energy pulsed plasma needle. In: The 62nd Gaseous Electronics conference, Saratoga Springs, NY, USA
Laroussi M, Alexeff I, Richardson JP, Dyer FF (2002) The resistive barrier discharge. IEEE Trans Plasma Sci 30:158–159
Haapasalo M, Orstavik D (1987) In vitro infection and disinfection of dentinal tubules. J Dent Res 66:1375–1379
Guggenheim B, Giertsen E, Schupbach P, Shapiro S (2001) Validation of an in vitro biofilm model of supragingival plaque. J Dent Res 80:363–370
Murray BE (1990) The life and times of the enterococcus. Clin Microbiol Rev 3:46–65
Jett BD, Huycke MM, Gilmore MS (1994) Virulence of enterococci. Clin Microbiol Rev 7:462–478
Estrela C, Sydney GB, Figueiredo JA, Estrela CR (2009) A model system to study antimicrobial strategies in endodontic biofilms. J Appl Oral Sci 17:87–91
Sundqvist G, Figdor D (2003) Life as an endodontic pathogen. Ecological differences between the untreated and root-filled root canals. Endod Top 6:3–28
Jiang C, Chen MT, Gundersen MA (2009) Polarity-induced asymmetric effects of nanosecond pulsed plasma jets. J Phys D Appl Phys 42:232002
Lu X, Laroussi M (2006) Dynamics of an atmospheric pressure plasma plume generated by submicrosecond voltage pulses. J Appl Phys 100:063302
Sands BL, Ganguly BN, Tachibana K (2008) A streamer-like atmospheric pressure plasma jet. Appl Phys Lett 92:151503
Jiang C, Schaudinn C (2011) A curving bactericidal plasma needle. IEEE Trans Plasma Sci 39:2966–2967
Laroussi M, Leipold F (2004) Evaluation of the roles of reactive species, heat, and uv radiation in the inactivation of bacterial cells by air plasmas at atmospheric pressure. Int J Mass Spectrom 233:81–86
Lu X, Ye T, Cao YG, Sun ZY, Xiong Q, Tang ZY, Xiong ZL, Hu J, Jiang ZH, Pan Y (2008) The roles of the various plasma agents in the inactivation of bacteria. J Appl Phys 104:053309
Acknowledgements
The authors thank Dr. Shawn Anderson for the donation of the tooth specimens for the experiments. This work is supported by the National Institute of Dental and Craniofacial Research (NIDCR), one of the National Institutes of Health (NIH) in the U.S. Department of Health and Human Services.
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Jiang, C., Schaudinn, C., Jaramillo, D.E., Gundersen, M.A., Costerton, J.W. (2012). A Sub-microsecond Pulsed Plasma Jet for Endodontic Biofilm Disinfection. In: Machala, Z., Hensel, K., Akishev, Y. (eds) Plasma for Bio-Decontamination, Medicine and Food Security. NATO Science for Peace and Security Series A: Chemistry and Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-2852-3_14
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DOI: https://doi.org/10.1007/978-94-007-2852-3_14
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