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How to Breed Diatoms: Examination of Two Species with Contrasting Reproductive Biology

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The Science of Algal Fuels

Part of the book series: Cellular Origin, Life in Extreme Habitats and Astrobiology ((COLE,volume 25))

Abstract

The diatoms have become a focus of attention as a potential resource for various industrial applications including biofuel production. Initially, progress can be made by screening species held in culture collections or isolated casually from natural samples. To make significant further advances, however, genetic modification (improvement) of promising species will be necessary, by selecting for economically valuable traits. Most diatoms are sexual organisms, and their genetic manipulation via classical plant breeding methods is desirable and possible, alongside modern approaches, for example, transformation. Here we explore the practicalities of diatom breeding by considering experimental control of sex (in the context of the whole life cycle) in two diatom species, Cyclotella meneghiniana and Seminavis robusta. These diatoms exhibit two different kinds of reproductive system, which are largely representative of the groups to which they belong, that is, the centric and pennate diatoms, respectively. We emphasize that efficient exploration of the potential of sexual breeding will also depend largely on progress in understanding many other fundamental aspects of diatom biology, including species-level taxonomy, population studies and biogeography. Unfortunately, the first genomic models, the centric Thalassiosira pseudonana and the pennate Phaeodactylum tricornutum, cannot be involved in investigations of the fundamentals of breeding behaviour in diatoms since, so far at least, there is no indication that these diatoms have a sexual phase in their life cycles.

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 References

  • Alverson AJ, Beszteri B, Julius ML, Theriot EC (2011) The model marine diatom Thalassiosira pseudonana likely descended from a freshwater ancestor in the genus Cyclotella. BMC Evol Biol 11:125. doi:10.1186/1471-2148-11-125

    Article  Google Scholar 

  • Amato A, Orsini L, D’Alelio D, Montresor M (2005) Life cycle, size reduction patterns, and ultrastructure of the pennate planktonic diatom Pseudo-nitzschia delicatissima (Bacillariophyceae). J Phycol 41:542–556

    Article  Google Scholar 

  • Armbrust EV (2009) The life of diatoms in the world’s oceans. Nature 459:185–192

    Article  CAS  Google Scholar 

  • Armbrust EV, Berges JA, Bowler C et al (2004) The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism. Science 306:79–86

    Article  CAS  Google Scholar 

  • Beszteri B, Ács É, Medlin LK (2005) Ribosomal DNA sequence variation among sympatric strains of the Cyclotella meneghiniana complex (Bacillariophyceae) reveals cryptic diversity. Protist 156:317–333

    Article  CAS  Google Scholar 

  • Bowler C, Allen AE, Badger JH et al (2008) The Phaeodactylum genome reveals the evolutionary history of diatom genomes. Nature 456:239–244

    Article  CAS  Google Scholar 

  • Bozarth A, Maier U-G, Zauner S (2009) Diatoms in biotechnology: modern tools and applications. Appl Microbiol Biotechnol 82:195–201

    Article  CAS  Google Scholar 

  • Chepurnov VA, Mann DG (2004) Auxosporulation of Licmophora communis (Bacillariophyta) and a review of mating systems and sexual reproduction in araphid pennate diatoms. Phycol Res 52:1–12

    Article  Google Scholar 

  • Chepurnov VA, Mann DG, Vyverman W, Sabbe K, Danielidis DB (2002) Sexual reproduction, mating system and protoplast dynamics of Seminavis (Bacillariophyceae). J Phycol 38:1004–1019

    Article  Google Scholar 

  • Chepurnov VA, Mann DG, Sabbe K, Vyverman W (2004) Experimental studies on sexual reproduction in diatoms. Int Rev Cytol 237:91–154

    Article  CAS  Google Scholar 

  • Chepurnov VA, Mann DG, von Dassow P, Armbrust EV, Sabbe K, Dasseville R, Vyverman W (2006) Oogamous reproduction, with two-step auxosporulation, in the centric diatom Thalassiosira punctigera (Bacillariophyta). J Phycol 42:845–858

    Article  Google Scholar 

  • Chepurnov VA, Mann DG, von Dassow P, Vanormelingen P, Gillard J, Inzé D, Sabbe K, Vyverman W (2008) In search of new tractable diatoms for experimental biology. Bioessays 30:692–702

    Article  Google Scholar 

  • Chepurnov VA, Chaerle P, Roef L, Van Meirhaeghe A, Vanhoutte K (2011) Classical breeding in diatoms: scientific background and practical perspectives. In: Seckbach J, Kociolek JP (eds) The diatom world. Springer, Dordrecht, pp 167–194

    Chapter  Google Scholar 

  • Davidovich NA, Bates SS (1998) Sexual reproduction in the pennate diatoms Pseudo-nitzschia multiseries and P. pseudodelicatissima (Bacillariophyceae). J Phycol 34:126–137

    Article  Google Scholar 

  • Davidovich NA, Mouget J-L, Gaudin P (2009) Heterothallism in the pennate diatom Haslea ostrearia (Bacillariophyta). Eur J Phycol 44:251–261

    Article  CAS  Google Scholar 

  • Desikachary TV, Rao VNR (1973) Studies on Cyclotella meneghiniana Kütz. III. The frustule. Proc Indian Acad Sci 77:78–92

    Google Scholar 

  • Drebes G (1977) Sexuality. In: Werner D (ed) The biology of diatoms: botanical monographs, vol 13. Blackwell Scientific Publications, Oxford, pp 250–283

    Google Scholar 

  • Dusenbery DB (2000) Selection for high gamete encounter rates explains the success of male and female mating types. J Theor Biol 202:1–10

    Article  CAS  Google Scholar 

  • Frenkel J, Gillard J, Vyverman W, Pohnert G (2012) Investigation of the mating pathway in the diatom Seminavis robusta. In: Sabbe K, Van de Vijver B, Vyverman W (eds) Twentysecond International Diatom Symposium, Aula Academica, Ghent, 26–31. Abstracts VLIZ Special Publication 58:174

    Google Scholar 

  • Geitler L (1932) Der Formwechsel der pennaten Diatomeen. Arch Protistenkd 78:1–226

    Google Scholar 

  • Geitler L (1973) Auxosporenbildung und Systematik bei pennaten Diatomeen und die Cytologie von Cocconeis-Sippen. Österr Bot Z 122:299–321

    Article  Google Scholar 

  • Geitler L (1985) Automixis bei pennaten Diatomeen. Plant Syst Evol 150:303–306

    Article  Google Scholar 

  • Gepts P, Hancock J (2006) The future of plant breeding. Crop Sci 46:1630–1634

    Article  Google Scholar 

  • Gillard J, Devos V, Huysman MJ, De Veylder L, D’Hondt S, Martens C, Vanormelingen P, Vannerum K, Sabbe K, Chepurnov VA, Inzé D, Vuylsteke M, Vyverman W (2008) Physiological and transcriptomic evidence for a close coupling between chloroplast ontogeny and cell cycle progression in the pennate diatom Seminavis robusta. Plant Physiol 148:1394–1411

    Article  CAS  Google Scholar 

  • Goodman MM (2004) Plant breeding requirements for applied molecular biology. Crop Sci 44:1913–1914

    Article  Google Scholar 

  • Griffiths AJF, Gelbart WM, Miller JH, Lewontin RC (1999) Modern genetic analysis. W. H. Freeman and Company, New York

    Google Scholar 

  • Grossman AR (2005) Paths toward algal genomics. Plant Physiol 137:410–427

    Article  CAS  Google Scholar 

  • Grossman AR (2007) In the grip of algal genomics. Adv Exp Med Biol 616:54–76

    Article  Google Scholar 

  • Guillard RRL, Lorenzen CJ (1972) Yellow-green algae with chlorophyllide c. J Phycol 8:10–14

    CAS  Google Scholar 

  • Gurian-Sherman D (2009) Failure to yield, evaluating the performance of genetically engineered crops. Union of Concerned Scientists, UCS Publications. http://www.ucsusa.org

  • Håkansson H, Chepurnov VA (1999) A study of variation in valve morphology of the diatom Cyclotella meneghiniana in monoclonal cultures: effect of auxospore formation and different salinity conditions. Diatom Res 14:251–272

    Article  Google Scholar 

  • Iyengar OP, Subrahmanyan R (1944) On reduction division and auxospore formation in Cyclotella meneghiniana. J Indian Bot Soc 23:125–152

    Google Scholar 

  • Jewson DH (1992) Life cycle of a Stephanodiscus sp. (Bacillariophyta). J Phycol 28:856–866

    Article  Google Scholar 

  • Jewson DH, Granin NG, Zhdanov AA, Gorbunova LA, Bondarenko NA, Gnatovsky RY (2008) Resting stages and ecology of the planktonic diatom Aulacoseira skvortzowii in Lake Baikal. Limnol Oceanogr 53:1125–1136

    Article  Google Scholar 

  • Knight J (2003) Crop improvement: a dying breed. Nature 421:568–570

    Article  CAS  Google Scholar 

  • Kroth P (2007) Molecular biology and the biotechnological potential of diatoms. Adv Exp Med Biol 616:23–33

    Article  Google Scholar 

  • Lebeau T, Robert J-M (2003) Diatom cultivation and biotechnologically relevant products. Appl Microbiol Biotech 60:612–632

    CAS  Google Scholar 

  • Mann DG (1999) The species concept in diatoms (Phycological Reviews 18). Phycologia 38:437–495

    Article  Google Scholar 

  • Mann DG (2011) Size and sex. In: Seckbach J, Kociolek JP (eds) The diatom world. Springer, Dordrecht, pp 147–166

    Google Scholar 

  • Mann DG, Chepurnov VA (2004) What have the Romans ever done for us? The past and future contribution of culture studies to diatom systematics. Nova Hedwigia 79:237–291

    Article  Google Scholar 

  • Mann DG, Chepurnov VA (2005) Auxosporulation, mating system, and reproductive isolation in Neidium (Bacillariophyta). Phycologia 44:335–350

    Article  Google Scholar 

  • Mann DG, Chepurnov VA, Droop SJM (1999) Sexuality, incompatibility, size variation and pre­ferential polyandry in natural populations and clones of Sellaphora pupula (Bacillariophyta). J Phycol 35:152–170

    Article  Google Scholar 

  • Mann DG, Evans KM, Chepurnov VA, Nagai S (2009) Morphology and formal description of Sellaphora bisexualis, sp. nov. (Bacillariophyta). Fottea 9:199–209

    Google Scholar 

  • Mascarelli AL (2009) Gold rush for algae. Nature 461:460–461

    Article  CAS  Google Scholar 

  • Mata TM, Martins AA, Caetano NS (2010) Microalgae for biodiesel production and other applications: a review. Renew Sust Energy Rev 14:217–232

    Article  CAS  Google Scholar 

  • Murphy D (2007) Plant breeding and biotechnology. Cambridge University Press, Cambridge

    Google Scholar 

  • Pickett-Heaps J, Schmid A-M M, Edgar LA (1990) The cell biology of diatom valve formation. Prog Phycol Res 7:1–168

    CAS  Google Scholar 

  • Poulíčková A, Mann DG (2006) Sexual reproduction in Navicula cryptocephala (Bacillariophyceae). J Phycol 42:872–886

    Article  Google Scholar 

  • Radakovits R, Jinkerson RE, Darzins A, Posewitz MC (2010) Genetic engineering of algae for enhanced biofuel production. Eukar Cell 9:486–501

    Article  CAS  Google Scholar 

  • Rao VNR (1970) Studies on Cyclotella meneghiniana Kütz: I. Sexual reproduction and auxospore formation. Proc Indian Acad Sci 72:285–287

    Google Scholar 

  • Rao VNR (1978a) Studies on Cyclotella meneghiniana Kütz. IV. Progressive diminution in cell size. Proc Indian Acad Sci 87B:1–15

    CAS  Google Scholar 

  • Rao VNR (1978b) Size dependent behaviour of the diatom Cyclotella meneghiniana. Curr Sci (India) 47:740–741

    Google Scholar 

  • Roshchin AM (1989) Proyavlenie intsukhta u diatomovoj vodorosli Synedra tabulata. Zh Obshch Biol 50:412–416

    Google Scholar 

  • Roshchin AM (1994) Zhiznennye Tsikly Diatomovykh Vodoroslej [Diatom life cycles]. Naukova Dumka, Kiev

    Google Scholar 

  • Roshchin AM, Chepurnov VA (1994) Allogamnyj polovoj protsess i gaploidnyj partenogenez u dvudomnoj vodorosli Licmophora ehrenbergii (Kütz.) Grun. (Bacillariophyta). Algologia 4(4):3–10

    Google Scholar 

  • Round FE (1972) The problem of reduction of cell size during diatom cell division. Nova Hedwigia 23:291–303

    Google Scholar 

  • Round FE, Crawford RM, Mann DG (1990) The diatoms: biology and morphology of the genera. Cambridge University Press, Cambridge

    Google Scholar 

  • Rozumek KE (1968) Der Einfluss der Umweltfaktoren Licht un Temperatur auf die Ausbildung der Sexualstadien bei der pennaten Diatomee Rhabdonema adriaticum Kütz. Beitr Biol Pfl 44:365–388

    Google Scholar 

  • Sato S, Beakes G, Idei M, Nagumo T, Mann DG (2011) Novel sex cells and evidence for sex pheromones in diatoms. PLoS One 6(10):e26923

    Article  CAS  Google Scholar 

  • Schultz ME, Trainor FR (1968) Production of male gametes and auxospores in the centric diatoms Cyclotella meneghiniana and C. cryptica. J Phycol 4:85–88

    Article  Google Scholar 

  • Sheehan J, Dunahay T, Benemann J, Roessler P (1998). A look back at the U.S. Department of energy’s aquatic species program: biodiesel from algae. TP-580-24190, National Renewable Energy Laboratory, Golden

    Google Scholar 

  • Shirokawa Y, Wakamoto Y, Shimada M (2010) Limited effects of cell size on gamete differentiation and sexual reproduction of Cyclotella meneghiniana. In: Abstracts presented at 21st international diatom symposium, St. Paul, Minnesota, USA, 29 Aug–3 Sept 2010, p 63

    Google Scholar 

  • Sieracki M, Poulton N, Crosbie N (2005) Automated isolation techniques for microalgae. In: Andersen RA (ed) Algal culturing techniques. Elsevier Academic Press, Burlington, pp 101–116

    Google Scholar 

  • Sleper DA, Poehlman JM (2006) Breeding field crops, 5th edn. Blackwell Publishing, Ames

    Google Scholar 

  • Snape JW (2004) Challenges of integrating conventional breeding and biotechnology: a personal view! In: Proceedings of the 4th international crop science Congress, Brisbane, Australia, 26 Sept–1 Oct 2004. http://www.cropscience.org.au/icsc2004/

  • Theriot EC, Ashworth M, Ruck E, Nakov T, Jansen RK (2010) A preliminary multigene phylogeny of the diatoms (Bacillariophyta): challenges for future research. Plant Ecol Evol 143:278–296

    Article  Google Scholar 

  • Trobajo R, Mann DG, Chepurnov VA, Clavero E, Cox EJ (2006) Taxonomy, life cycle and auxosporulation of Nitzschia fonticola (Bacillariophyta). J Phycol 42:1353–1372

    Article  CAS  Google Scholar 

  • Vanormelingen P, Chepurnov VA, Mann DG, Sabbe K, Vyverman W (2008) Genetic divergence and reproductive barriers among morphologically heterogeneous sympatric clones of Eunotia bilunaris sensu lato (Bacillariophyta). Protist 159:73–90

    Article  CAS  Google Scholar 

  • Vaulot D, Chisholm SW (1987) Flow cytometric analysis of spermatogenesis in the diatom Thalassiosira weissflogii (Bacillariophyceae). J Phycol 23:132–137

    CAS  Google Scholar 

  • von Stosch HA (1956) Entwicklungsgeschichtliche Untersuchungen an zentrischen Diatomeen II. Geschlechtszellenreifung, Befruchtung und Auxosporenbildung einiger grundbewohnender Biddulphiaceen der Nordsee. Arch Mikrobiol 23:327–365

    Article  Google Scholar 

  • von Stosch HA (1958) Kann die oogame Araphidee Rhabdonema adriaticum als Bindeglied zwischen den beiden grossen Diatomeen gruppen angesehen werden? Ber Deut Bot Ges 71:241–249

    Google Scholar 

  • von Stosch HA (1965) Manipulierung der Zellgrösse von Diatomeen im experiment. Phycologia 5:21–44

    Article  Google Scholar 

  • Wiese L (1969) Algae. In: Metz CB, Monroy A (eds) Fertilization, comparative morphology, biochemistry and immunology, vol II. Academic, London, pp 135–188

    Google Scholar 

  • Wijffels RH, Barbosa MJ (2010) An outlook on microalgal biofuels. Science 329:796–799

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. SBAE Industries nv, Hooiwege 40, 9940, Sleidinge, Belgium

    Victor A. Chepurnov, Peter Chaerle & Koen Vanhoutte

  2. Royal Botanic Garden, Edinburgh, EH3 5LR, Scotland, UK

    David G. Mann

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  1. Victor A. Chepurnov
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  2. Peter Chaerle
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  3. Koen Vanhoutte
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  4. David G. Mann
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Correspondence to Victor A. Chepurnov .

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Editors and Affiliations

  1. Gulf Specimen Marine Laboratory, Clark Drive 222, Panacea, 32346, Florida, USA

    Richard Gordon

  2. University of Jerusalem, Mevo Hadas 20, Efrat, 90435, Israel

    Joseph Seckbach

Additional information

Dedicated to the memory of Frank E. Round (1927–2010) and Alexei M. Roshchin (1935–2010), two scientists who contributed greatly to our knowledge of diatoms.

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Chepurnov, V.A., Chaerle, P., Vanhoutte, K., Mann, D.G. (2012). How to Breed Diatoms: Examination of Two Species with Contrasting Reproductive Biology. In: Gordon, R., Seckbach, J. (eds) The Science of Algal Fuels. Cellular Origin, Life in Extreme Habitats and Astrobiology, vol 25. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5110-1_18

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