Abstract
In nature growth of microorganisms is generally a batch phenomenon. Individual, microbial cells are completely self-sufficient and proliferate to the extent permitted by nutrients and other conditions in their environment. This is mimicked by batch fermentation methods in which a culture of microorganisms is inoculated into a rich nutrient medium and allowed to grow to its maximum density limited by nutrient depletion and waste product accumulation. From one point of view, microorganisms have evolved specifically to meet the changing conditions that are found in the batch fermentation process. Conversely, cells of higher animals have evolved as integral components of a multicellular organism such that each cell of that organism exists in a highly regulated environment that provides constant levels of nutrients, metabolic products and complex hormonal and biochemical control mechanisms to allow optimum differentiated cellular functions.
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References
Patterson, M.K., Jr., TCA Manual, 1, 243 (1975)
Himmelfarb, P., Thayer, P.S., and Martin, H.E., Science, 164, 555 (1969).
Knazek, R.A., Gullino, P.M., Kohler, P.O., and Dedrick, R.L., Science, 178, 65 (1972).
Tolbert, W.R. and Feder, J., in Annual Reports on Fermentation Processes, Vol. 6, (G.T. Tsao and M.C. Flickinger, eds.), Academic Press, New York, pp. 35–74 (1983).
Feder, J. and Tolbert, W.R., eds. Large-Scale Mammalian Cell Culture, Academic Press, New York, p. 159 (1985).
Reuveney, S., Velez, D., Miller, L. and MacMillan, J.D., J. Immunol. Methods, 86, 61–69 (1986).
Feder, J., in Advances in Biotechnological Processes, Vol. 7, Upstream Processes — Equipment and Technique, (A. Mizrahi, ed.), Alan R. Liss, Inc. (in press).
Ratafia, M., Bio/Technology, 5, 692–693, (1987).
Lydersen, J. of Tissue Culture Meth, 4, 147–154 (1983).
Hopkinson, J., in “Immobilized Cells and Organells”, Vol I, (B. Mattiasson, ed.), CRC Press, Boca Raton, FL, pp. 89–99 (1983).
Van Wezel, A.L., in Research Needs in Non-Conventional Bioprocesses, (D.J. Fink, L.M. Curran, B.R. Allen, eds.) Bettelle Press, Columbus, OH, pp. 86–90 (1985).
Sato, S., Kawamura, K. and Fujiyoshi, N., J. of Tissue Culture Meth., 4, 167–171 (1983).
Fujiyoshi, N. personal communication.
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© 1989 Kluwer Academic Publishers
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Tolbert, W.R., Prior, C.P. (1989). Perfusion Culture. In: Miller, A.O.A. (eds) Advanced Research on Animal Cell Technology. NATO ASI Series, vol 156. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0875-8_9
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DOI: https://doi.org/10.1007/978-94-009-0875-8_9
Publisher Name: Springer, Dordrecht
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