Skip to main content
SpringerLink
Log in

Tumor Associated Antigens and Oncogene Products Defined by Monoclonal Antibodies

  • Chapter
Application of Monoclonal Antibodies in Tumor Pathology

Part of the book series: Developments in Oncology ((DION,volume 50))

  • 52 Accesses

Abstract

The advent of hybridoma/monoclonal antibody technology in 1975 (1) and gene, cloning procedures have drastically influenced both the understanding and practice of pathology. Using these methodologies and reagents, applications have been developed in the research laboratory, distributed to the clinical setting, and now often impact on the diagnosis and management of human disease. It is the authors’ intent to focus this chapter on some recently developed monoclonal antibody applications in human pathology using immunohistochemical techniques. The text will be roughly divided into three parts: background information and general principles, practical applications, and monoclonal antibodies as probes to investigate tumor biology and oncogene expression.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Kohler M, Milstein C: Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495, 1975.

    Article  PubMed  CAS  Google Scholar 

  2. Sell S: Cancer Markers, Diagnostic and Developmental Significance. Clifton, NJ, Human Press, 1980.

    Google Scholar 

  3. Stefarti M: Enzymes, isoenzymes, and enzyme variants in the diagnosis of cancer. Cancer 55: 1391, 1985.

    Google Scholar 

  4. Van Lente F, Shamberger RJ: Defining a role for enzymes in cancer diagnosis. Diag Med 47, June 1983.

    Google Scholar 

  5. Wu JT: Applications of enzymes: tumor markers, new enzymes, and atypical isoenzymes. J Med Technol 7: 438, 1985.

    Google Scholar 

  6. Mirecki DM, Jordan VC: Steroid hormone receptors and breast cancer. Lab Med 16: 287, 1985.

    CAS  Google Scholar 

  7. Hakomori S: Tumor-associated carbohydrate antigens. Ann Rev Immunol 2: 103, 1984.

    Article  CAS  Google Scholar 

  8. Ohosh BC (ed): Tumor Associated and Other Related Markers: McGraw Hill Publishers (in press).

    Google Scholar 

  9. Colcher D, Horan Hand P, Nuti M et al.: A spectrum of monoclonal antibodies reactive with mammary tumor cells. Proc Natl Acad Sci USA 78: 3199, 1981.

    Article  PubMed  CAS  Google Scholar 

  10. Nuti M, Teramoto YA, Mariani-Costantini R et al.: A monoclonal antibody (B72.3) defines patterns of distribution of a novel tumor-associated antigen in human mammary carcinoma cell populations. Int J Cancer 29: 539, 1982.

    Article  PubMed  CAS  Google Scholar 

  11. Stragmignoni D, Bowen R, Atkinson B et al.: Differential reactivity of monoclonal antibodies with human colon adenocarcinomas and adenomas. Int J Cancer 31: 543, 1983.

    Article  Google Scholar 

  12. Horan Hand P, Colcher D, Salomon D, Ridge J, Noguchi P, Schlom J: Influence of spatial configuration of carcinoma cell populations on the expression of fa tumor-associated glycoprotein. Cancer Res, 45: 833–840, 1985.

    PubMed  CAS  Google Scholar 

  13. Johnson VG, Schlom J, Paterson AJ et al.: Analysis of a human tumor- associated glycoprotein (TAG-72) identified by monoclonal antibody B72.3. Cancer Res, 46: 850, 1986.

    PubMed  CAS  Google Scholar 

  14. Friedman E, Thor A, Horan Hand P, Schlom J: Surface expression of tumor-associated antigens in primary cultured human colonic epithelial cells from carcinomas, benign tumors and normal tissues. Cancer Res, 45: 5648, 1985.

    PubMed  CAS  Google Scholar 

  15. Greiner J, Horan Hand P, Pestka S, Noguchi P, Fisher P, Colcher D, Schlom J: Selection and enhancement (by recombinant interferon) of carcinoma cell surface antigens using monoclonal antibodies. In: Cancer Cells 1: The Transformed Phenotype, pp. 285–292, Cold Spring Harbor Lab., 1984.

    Google Scholar 

  16. Colcher D, Zalutsky M, Kaplan W et al.: Radiolocalization of human mammary tumors in athymic mice by a monoclonal antibody. Cancer Res, 43: 736–742, 1983.

    PubMed  CAS  Google Scholar 

  17. Schlom J, Greiner J, Horan Hand P, Colcher D, Inghirami G, Weeks M, Pestka S, Fisher PB, Noguchi P, Kufe D: Monoclonal antibodies to breast cancer-associated antigens as potential reagents in the management of breast cancer. Cancer 54: 2777–2794, 1984.

    Article  PubMed  CAS  Google Scholar 

  18. Keenan AM, Colcher D, Larson SM, Schlom J: Radioimmunoscintigraphy of human colon cancer xenografts in mice with radioiodinated monoclonal antibody B72.3. J Nucl Med 25: 1197–1203.

    Google Scholar 

  19. Thor A, Ohuchi N, Szpak CA, Johnston WW, Schlom J: The distribution of oncofetal antigen TAG-72 defined by monoclonal antibody B72.3. Cancer Res (in press).

    Google Scholar 

  20. Thor A, Gorstein F, Ohuchi N, Szpak CA, Johnston WW, Schlom J: Monoclonal antibody B72.3 defines tumor associated antigen (TAG-72) in ovarian carcinoma. JNCI (in press).

    Google Scholar 

  21. Colcher D, Carrasquillo JA, Sugarbaker P, Bryant G, Esteban J, Simpson D, Thor A, Reynolds JR, Horowitz M, Larson SM, Schlom J: Biodistribution of monoclonal antibody B72.3 (I-131) in colon cancer patients. Proc of Society of Nuclear Medicine. P15, 1985.

    Google Scholar 

  22. Paterson AJ, Schlom J, Sears NF, Bennett J, Colcher D: A radioimmunoassay for the detection of a human tumor-associated glycoprotein (TAG- 72) using monoclonal antibody B72.3 Cancer Res (in press).

    Google Scholar 

  23. Johnston WW: The malignant pleural effusion: a review of cytopathological diagnoses on 584 specimens from 472 consecutive patients. Cancer 56: 905, 1984.

    Article  Google Scholar 

  24. Kufe D, Inghirami G, Abe M et al.: Differential reactivity of a novel monoclonal antibody (DF3) with human malignant versus benign breast tumors. Hybridoma, 3: 223, 1984.

    Article  PubMed  CAS  Google Scholar 

  25. Sekine H, Ohno T, Kufe DW: Purification and characterization of high molecular weight glycoprotein detectable in human milk and breast carcinoma. J Immunol, 135, 1985.

    Google Scholar 

  26. Lundy J, Thor A, Maenza R et al.: Monoclonal antibody DF3 correlates with tumor differentiation and hormone receptor status in breast cancer patients. Br Cancer Res and Treat, 5: 269, 1985.

    Article  CAS  Google Scholar 

  27. Szpak CA, Johnston WW, Lottich SC, Kufe D, Thor A, Schlom J: Patterns of reactivity of four novel monoclonal antibodies (B72.3, DF3, B1.1 and B6.2) with cells in human malignant and benign effusions. Acta Cytol, 28: 356, 1984.

    PubMed  CAS  Google Scholar 

  28. Johnston WW, Szpak CA, Lottich SC, Thor A, Schlom J: Use of a monoclonal antibody (B72.3) as an immunocytochemical adjunct to diagnosis of adenocarcinoma in human effusions. Cancer Res, 45: 1894–1900, 1985.

    PubMed  CAS  Google Scholar 

  29. Martin SE, Moshiri S, Thor A, Vilasi V, Chu EW, Schlom J: Identification of adenocarcinoma in cytospin preparations of effusions using monoclonal antibody B72.3. Am J Clin Path (in press).

    Google Scholar 

  30. Johnst on WW, Szpak CA, Thor A, Schlom J: The use of monoclonal antibody (B72.3) as a novel immunocytochemical adjunct for the diagnosis of carcinomas in fine needle aspiration biopsies. Human Path (in press).

    Google Scholar 

  31. Lundy J, Lozowski M, Mishriki Y: Monoclonal antibody B72.3 as a diagnostic adjunct in fine needle aspirates of breast masses. Ann Surg (in press).

    Google Scholar 

  32. Nuti M, Mottolese M, Viora M, Donnorso RP, Schlom J, Natali PG: Use of monoclonal antibodies to human breast tumor associated antigens in fine needle aspirate cytology. Int J Cancer (in press).

    Google Scholar 

  33. Szpak CA, Johnston WW, Roggli V et al.: The diagnostic distinction between malignaht mesothelioma of the pleura and adenocarcinoma of the lung defined by a monoclonal antibody (B72.3). Am J Path (in press).

    Google Scholar 

  34. Antman KH, Blum EH, Greenberger JS et al.: Multimodality therapy for malignant mesothelioma based on a study of natural history. Am J Med, 68: 356, 1980.

    Article  PubMed  CAS  Google Scholar 

  35. Legha SS, Muggia, FM: Pleural mesothelioma: clinical features and therapeutic implications. Ann Intern Med, 87: 613, 1977.

    PubMed  CAS  Google Scholar 

  36. Wang N, Huang SN, Gold P: Absence of carinoembryonic antigen-like material in mesothelioma. An immunohistochemical differentiation from other lung cancer. Cancer, 44: 937, 1979.

    Article  PubMed  CAS  Google Scholar 

  37. Corson JM, Pinkus GS: Mesothelioma: Profile of keratin proteins and carcinoembryonic antigen. An immunoperoxidase study of 20 cases and comparison with pulmonary adenocarcinomas. Am J Pathol, 108: 80, 1982.

    PubMed  CAS  Google Scholar 

  38. Said JW, Nash G, Tepper G et al.: Keratin proteins and carcinoembryonic antigen in lung carcinoma: An immunoperoxidase study of 54 cases, with ultrastructural correlations. Hum Pathol, 14: 70–76, 1983.

    Article  PubMed  CAS  Google Scholar 

  39. Holden J, Churg A: Immunohistochemical staining for keratin and carcinoembryonic antigen in the diagnosis of malignant mesothelioma. Am J Surg Pathol, 8: 277, 1984.

    Article  PubMed  CAS  Google Scholar 

  40. Liotta LA: Tumor invasion and metastases: role of the basement membrane. Am J Path, 117: 340, 1984.

    Google Scholar 

  41. Liotta LA, Rao CN, Barsky SH: Tumor invasion and the extracellular matrix. Lab Invest, 49: 636, 1983.

    PubMed  CAS  Google Scholar 

  42. Barsky SH, Rao CN, Hyams D, Liotta LA: Characterization of a laminin receptor from human breast carcinoma tissue. Breast Cancer Res Treat, 4: 181, 1984.

    Article  PubMed  CAS  Google Scholar 

  43. Barsky SH, Rao CN, Williams JE, Liotta LA: Laminin molecular domains which alter metastasis in a murine mode. J Clin Invest, 74: 0001, 1984.

    Article  Google Scholar 

  44. Liotta LA, Horan Hand P, Rao CN, Bryant G, Barsky SH, Schlom J: Monoclonal ntibodies to the human laminin receptor recognize structurally distinct sites. Expt Cell Res, 156: 117, 1985.

    Article  CAS  Google Scholar 

  45. Horan Hand P, Thor A, Schlom J, Rao CN, Liotta LA: Expression of laminin receptor in normal and carcinomatous human tissues as defined by a monoclonal antibody. Cancer Res, 45: 2713, 1985.

    Google Scholar 

  46. Land H, Parada L, Weinberg RA: Cellular oncogenes and multistep carcinogenesis. Science, 222: 771, 1983.

    Article  PubMed  CAS  Google Scholar 

  47. Shih TY, Weeks MO: Oncogenes and cancer: the p21 ras genes. Cancer Invest, 2: 109, 1984.

    Article  PubMed  CAS  Google Scholar 

  48. Pulciani S, Santos E, Lauver AV, Long LK, Aaronson SA, Barbacid M: Oncogenes in solid human tumours. Nature (London), 300:, 539–542, 1982.

    Article  CAS  Google Scholar 

  49. Kraus MH, Yuasa Y, Aaronson SA: A position 12-activated H-ras oncogene in all HS578T mammary carcinoma cells but not normal mammary cells of the same patient. Proc Natl Acad Sci USA, 81: 5384, 1984.

    Article  PubMed  CAS  Google Scholar 

  50. Fujita J, Srivastava SK, Kraus MH, Rhim JS, Tronick SR, Aaronson SA: Frequency of molecular alterations affecting ras protooncogenes in human urinary tract tumors. Proc Natl Acad Sci USA, 82: 3849, 1985.

    Article  PubMed  CAS  Google Scholar 

  51. Horan Hand P, Thor A, Wunderlich D et al.: Monoclonal antibodies of predefined specificity detect activated ras gene expression in human mammary and colon carcinomas. Proc Natl Acad Sci, 81: 5227, 1984.

    Article  Google Scholar 

  52. Thor A, Horan Hand P, Wunderlich D et al.: Monoclonal antibodies define differential ras gene expression in malignant and benign colonic disease states. Nature, 311: 562, 1984.

    Article  PubMed  CAS  Google Scholar 

  53. McCormick F, Clark BFC, La Cour TF, Kjeldgaard M, Norskov-Lauritsen L, Nyborg J: A model for ther tertiary structure of p21, the product of the ras oncogene. Science, 230: 78, 1985.

    Article  PubMed  CAS  Google Scholar 

  54. Viola MV, Tromowitz F, Oravez S, Deb S, Schlom J: ras oncogene p21 expression is increased in premalignant lesions and high grade bladder carcinoma. J Expt Med, 161: 1213, 1985.

    Article  CAS  Google Scholar 

  55. Feinberg A, Vogelstein B, Droller M, Baylin S, Nelkin, B: Mutations affecting the 12th amino acid of c-Ha-ras oncogene product occur infrequently in bladder cancer. Science, 220: 1175.

    Google Scholar 

  56. Fujita J, Yoshida O, Yuasa Y, Rhim J, Hatanaka M, Aaronson S: Ha-ras oncogenes are activated by somatic alterations in human urinary tract tumours. Nature, 309: 464, 1984.

    Article  PubMed  CAS  Google Scholar 

  57. Viola MJ, Tromowitz F, Oravez S, Deb S, Sinkel G, Lundy J, Horan Hand P, Thor A, Schlom J: Expression of ras oncogene p21 in prostate cancer. NEJM 314: 133, 1986.

    Article  PubMed  CAS  Google Scholar 

  58. Spandidos DA, Kerr IB: Elevated expression of the human ras oncogene family in premalignant and malignant tumours of the colorectum. Br J Cancer 49: 681, 1984.

    Article  PubMed  CAS  Google Scholar 

  59. Spandidos DA, Agnantis NJ: Human malignant tumors of the breast, as compared to their respective normal tissue have elevated expression of the Harvey ras oncogene. Anticancer Res, 4: 269, 1984.

    PubMed  CAS  Google Scholar 

  60. DeBortoli ME, Abou-Issa H, Haley BE, Cho-Chung YS: Amplified expression of P21 ras protein in hormone-dependent mammary carcinoma of humans and rodents. Biophys Res Commun, 127: 699, 1985.

    Article  CAS  Google Scholar 

  61. Gallick GE, Kurzrock R, Kloetzer WS, Arlinghaus RB, Gutterman JU: Expression of p21 ras in fresh primary and metastatic human colorectal tumors. Proc Natl Acad Sci USA, 82: 1795, 1985.

    Article  PubMed  CAS  Google Scholar 

  62. Horan Hand P, Thor A, Caruso A, Wunderlich D, Muraro R, Weeks MO, Vilasi V, Schlom J: Comparisons of qualitative and quantitative immunoassays for ras p21. In: Monoclonal Antibodies and Cancer Therapy p 23–36, Alan R. Liss, Inc., 1985.

    Google Scholar 

Download references

Authors
  1. A. Thor
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. Ohuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Horan Hand
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. O. Weeks
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. W. W. Johnston
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. C. A. Szpak
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. J. Schlom
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Pathology, University Medical Center, Nijmegen, The Netherlands

    Dirk J. Ruiter

  2. Department of Pathology, University Medical Center, Leiden, The Netherlands

    Gert Jan Fleuren  & Sven O. Warnaar  & 

Rights and permissions

Reprints and permissions

Copyright information

© 1987 Martinus Nijhoff Publishers, Dordrecht

About this chapter

Cite this chapter

Thor, A. et al. (1987). Tumor Associated Antigens and Oncogene Products Defined by Monoclonal Antibodies. In: Ruiter, D.J., Fleuren, G.J., Warnaar, S.O. (eds) Application of Monoclonal Antibodies in Tumor Pathology. Developments in Oncology, vol 50. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3299-9_16

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-3299-9_16

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-7981-5

  • Online ISBN: 978-94-009-3299-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation