Abstract
Primary cultures of beating rat heart muscle cells were shown to be a good model system for studies of iron uptake and intracellular iron metabolism. Iron uptake from several iron sources was rapid and ferritin was visualized (by electron microscopy) within 48–96 hours after addition of iron. Iron was distributed in various cellular fractions; an iron-containing cytosol component(s) (non-ferritin) that may represent a “pool” of iron was evident. The cultured rat myocytes were used to screen chelators that might become useful in therapy of human iron overload. One chelator, agrobactin, was significantly more effective than the drug deferoxamine in reduction of iron uptake from transferrin-iron by the cells; however, enterobactin increased iron uptake from this source. Deferoxamine inhibited incorporation of iron into ferritin and increased the amount of iron in the non-ferritin cytosol fraction. Therefore, some of these chelators may be useful probes in studies of intracellular iron metabolism.
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© 1982 D. Reidel Publishing Company
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Byers, B.R., Sciortino, C.V., Cox, P., Robinson, P. (1982). Iron Uptake and Intracellular Iron Distribution in Cultured Rat Heart Cells: Effects of Iron Chelators. In: Dunford, H.B., Dolphin, D., Raymond, K.N., Sieker, L. (eds) The Biological Chemistry of Iron. NATO Advanced Study Institutes Series, vol 89. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-7882-9_8
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DOI: https://doi.org/10.1007/978-94-009-7882-9_8
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