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Calcium Complexities: New Fluorescence Techniques for Probing Mitochondria and Other Subcellular Compartments

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Calcium: The Molecular Basis of Calcium Action in Biology and Medicine

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Abstract

As evident in all of the chapters throughout this book, Ca2+ is an essential regulator of various cellular functions, including muscle contraction and the release of neurotransmitters and hormones. However, the question can be asked: How can a ubiquitous signal like Ca2+ generate so many different physiological responses while maintaining selectivity as a cellular signal? (Berridge, 1997b). Since the advent of Ca2+-sensitive fluorophores and fluorescence microscopy, researchers have been able to visualize Ca2+ signaling in living cells. These studies have revealed complexities in temporal and spatial regulation of Ca2+ signaling, which appear to hold the key to how Ca2+ can act as both a ubiquitous and selective signal (Berridge, 1997b). Furthermore, confocal and electron microscopy studies have shown that the locality of key Ca2+ transporters and the concentration of Ca2+ in sub-cellular compartments is not homogenous, with consequent physiological implications. In this chapter we will discuss the spatial and temporal complexities of Ca2+ signaling, in particular recent studies using confocal, multi-photon and high-speed fluorescence microscopy as well as “caged” regulators of intracellular Ca2+.

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Authors and Affiliations

  1. The School of Pharmacy, The University of Queensland, St. Lucia, Queensland, Australia, 4072

    Gregory R. Monteith

  2. The School of Pharmacy, The University of Sydney, New South Wales, Australia, 2006

    Vadim N. Dedov & Basil D. Roufogalis

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  1. Université Libre de Bruxelles, Belgium

    Roland Pochet

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Monteith, G.R., Dedov, V.N., Roufogalis, B.D. (2000). Calcium Complexities: New Fluorescence Techniques for Probing Mitochondria and Other Subcellular Compartments. In: Pochet, R., Donato, R., Haiech, J., Heizmann, C., Gerke, V. (eds) Calcium: The Molecular Basis of Calcium Action in Biology and Medicine. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0688-0_42

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