Abstract
This report shows a method to establish a recombinant CHO cell line that grows and secretes human antithrombin-III (AT-III) in a serum-free medium. At first, serum-dependent cell lines were obtained by introducing AT-III cDNA and following MTX-treatments. Then the cells were transferred to a serum-free medium, GCM001, and cultivated for several weeks. Serum- independent cells were obtained through cultivation in GCM001, and some cell lines showed a little greater productivity of AT-III in GCM001 than serum-dependent, parental cells in serum-containing medium. AT-III produced by both serum-independent and serum-dependent cells had the same specific activity, but heterogeneity in glycosylations was observed between them.
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© 1992 Springer Science+Business Media Dordrecht
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Asakura, E. et al. (1992). Production of Human Antithrombin-III in a Serum-Free Culture of CHO Cells. In: Murakami, H., Shirahata, S., Tachibana, H. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2844-5_47
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DOI: https://doi.org/10.1007/978-94-011-2844-5_47
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5263-4
Online ISBN: 978-94-011-2844-5
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