Skip to main content
SpringerLink
Log in

Factor VII clotting activity

  • Chapter
ECAT Assay Procedures A Manual of Laboratory Techniques

  • 86 Accesses

Abstract

Factor VII is a single-chain, vitamin K-dependent glycoprotein of molecular weight 47 000–50 0001–5. Its plasma concentration is about 250–700ng/ml (5–15nmol/L). The complex of the zymogen factor VII with tissue thromboplastin and Ca2+ has been proposed as an efficient activator of factor X7,8 Factor VII can be converted to the two-chain α-VIIa by hydrolysis of an Arg-Ile bond by factors Xa, IXa, XIIa or thrombin1,2,6,10 The coagulant activity of α-VIIa is 100-fold increased compared to single-chain factor VII8. Further cleavage of α-VIIa at the heavy chain destroys coagulant activity9. The complex of factor VII/VIIa with tissue factor also activates factor IX to IXa11,12. Some authors13,14 virtually deny any physiologically relevant enzymatic activity to the complex of tissue factor with non-activated factor VII. Their concept of extrinsic activation requires the presence of factor VIIa in trace amounts. Factor Xa will then be generated by the factor VIIa/tissue factor complex (and also by reactions of the intrinsic coagulation pathway) and activate the thromboplastin bound zymogen factor VII to VIIa. This potent amplification loop is subjected to complex regulation mechanisms15.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Broze GJ, Majerus PW Purification and properties of human coagulation factor VII. J Biol Chem 1980; 255: 1242–7.

    PubMed  CAS  Google Scholar 

  2. Bajaj SP, Rapaport SI, Brown SF Isolation and chracterization of human factor VII. Activation of factor VII by factor Xa. J Biol Chem 1981; 256: 253–9.

    PubMed  CAS  Google Scholar 

  3. Radcliffe R.D, Nermerson Y. The activation and control of factor VII by activated factor X and thrombin. J Biol Chem 1975; 250: 338–95.

    Google Scholar 

  4. Broze GJ, Majerus PW Human VII. Meth Enzymol 1982; 80: 228–34.

    Article  Google Scholar 

  5. Fair DS Quantitation of factor VII in the plasma of normal and warfarin-treated individuals by radioimmunoassay. Blood 1983; 62: 784–91.

    PubMed  CAS  Google Scholar 

  6. Masys D.R, Bajaj SP, Rapaport SI Activation of human factor VII by activated factors IX and X. J Biol Chem 1981; 256: 253–9.

    Google Scholar 

  7. Radcliffe R, Nernerson Y Activation and control of factor X and thrombin: Isolation and characterization of a high chain form of factor VII. J Biol Chem 1972; 250:388–95.

    Google Scholar 

  8. Bach R, Oberdick J, Nernerson Y. Immunoaffinity purification of bovine factor VII. Blood 1984; 63: 393–8.

    PubMed  CAS  Google Scholar 

  9. Radcliffe R, Nermerson Y Mechanism of action of bovine factor VII: Products of cleavage by factor Xa. J Biol Chem 1976; 251: 4797–802.

    CAS  Google Scholar 

  10. Radcliffe RD, Bagdasarian A, Colman R, Nemerson Y. Activation of bovine factor VII by Hageman factor fragments. Blood 1977; 50: 611–17.

    PubMed  CAS  Google Scholar 

  11. Jesty J, Morrison SA. The activation of factor IX by tissue factor-factor VII in a bovine plasma system lacking factor X. Thromb Res 1983; 32: 171–81.

    Article  PubMed  CAS  Google Scholar 

  12. Born VJJ, Reinalda-Poot JH, Cupers R, Bertina RM Extrinsic activation of human blood coagulation factors IX and X. Thromb Haemostas 1990; 63: 224–30.

    Google Scholar 

  13. Rao LVM, Rapaport SI, Bajaj SP Activation of human factor VII in the initiation of tissue factor-dependent coagulation. Blood 1986; 68: 685–91.

    PubMed  CAS  Google Scholar 

  14. Rao LVM, Rapaport SI Activation of factor VII bound to tissue factor: a key early step in the tissue factor pathway of blood coagulation. Proc Natl Acad Sci USA 1988; 85: 6687–91.

    Article  PubMed  CAS  Google Scholar 

  15. Sanders NL, Bajaj SP, Zivelin A, Rapaport SI Inhibition of tissue factor/factor VII activity in plasma requires factor X and an additional plasma component. Blood 1985; 66: 204–12.

    PubMed  CAS  Google Scholar 

  16. Bloom AL Inherited disorders of blood coagulation. In: Bloom A.L, Thomas DP, eds. Haemostasis and Thrombosis, 2nd ed. Edinburgh: Churchill Livingstone, 1987; 393–441.

    Google Scholar 

  17. Roberts HR, Foster PA Inherited disorders of prothrombin conversion. In: Colman RW, Hirsh J, Marder VJ, Salzman EW, eds. Hemostasis and thrombosis, 2nd ed. Philadelphia: Lippincott, 1987; 162–81.

    Google Scholar 

  18. Meade TW, North WRS, Chakrabarti R, Stirling Y, Haines AP, Thompson SG Haemostatic function and cardiovascular death: early results of a prospective study. Lancet 1980; 1: 1050–4.

    Article  PubMed  CAS  Google Scholar 

  19. Meade TW Factor VII and ischaemic heart disease: epidemiological evidence. Haemostasis 1983; 13: 178–85.

    PubMed  CAS  Google Scholar 

  20. Meade TW, Mellows S, Brozovic M, Miller SG, Chakrabarti RR, North WRS, Haines AP, Stirling Y, Imeson ID, Thomson SG Haemostatic function and ischaemic heart disease: Principal results of the Northwick Park Heart Study. Lancet 1986; 2: 533–7.

    Article  PubMed  CAS  Google Scholar 

  21. Meade TW The epidemiology of haemostatic and other variables in coronary artery disease. In: Verstraete M, Vermylen J, Lijnen HR, Amout J, eds. Thrombosis and haernostasis. Leuven University Press 1987; 37–60.

    Google Scholar 

  22. Miller GJ, Seghatchian MJ, Walter SJ, Howarth DJ, Thompson SG, Esnouf MP, Meade TW An association between the factor VII coagulant activity and thrombin activity induced by surface/cold exposure of normal human plasma. Br J Haematol 1986; 62: 379–84.

    Article  PubMed  CAS  Google Scholar 

  23. Scarabin PY, Bonithon-Kopp C, Bara L, Malmejac A, Guize L, Samama M Factor VII activation and menopausal status. Thromb Res 1990; 57: 227–34.

    Article  PubMed  CAS  Google Scholar 

  24. Dalaker K, Hjermann I, Prydz H A novel form of factor VII in plasma from men at risk for cardiovascular disease. Br J Haematol 1985; 61: 315–22.

    Article  PubMed  CAS  Google Scholar 

  25. Scarabin PY, Van Dreden P, Bonithon-Kop C, Orssaud G, Bara L, Conard J, Samama M Age related changes in factor VII activation in healthy women. Clin Sci 1988; 75: 341–3.

    PubMed  CAS  Google Scholar 

  26. Hoffman C, Shak A, Sodums M, Hultin MB Factor VII activity state in coronary artery disease. J Lab Clin Med 1988; 111: 475–81.

    PubMed  CAS  Google Scholar 

  27. Miller GJ, Walter SJ, Stirling Y, Thompson SG, Esnouf MP, Meade TW Assay of factor VII activity by two techniques: evidence for increased conversion of VII to alpha Vila in hyperlipaemia, with possible implications for ischaemic heart disease. Br J Haematol 1985;59: 249–58.

    Article  PubMed  CAS  Google Scholar 

  28. Czendlik C, Lämmle B, Duckert F. Cold promoted activation and factor XII, prekallikrein and C1-inhibitor. Thromb Haemostas 1985; 53: 242–4.

    CAS  Google Scholar 

  29. Gjnnaess H Cold promoted activation of factor VII. Occurrence and relation to sex hormones and antifertility compounds. Gynaecol Invest 1973; 4: 61–72.

    Article  Google Scholar 

  30. Seligsohn U, Osterud B, Brown SF, Griffin JH, Rapaport SI Activation of human factor VII in plasma and purified systems. Roles of activated factor IX, kallikrein and activated factor XII. J Clin Invest 1979; 64: 1056–65.

    Article  PubMed  CAS  Google Scholar 

  31. Barrowcliffe TW, Curtis AD Principles of bioassay. In: Bloom AL, Thomas DP, eds. Haemostasis and thrombosis. Edinburgh: Churchill Livingstone, 1987; 996–1004.

    Google Scholar 

  32. Finney DJ Statistical method in biological assay, 3rd ed. London: Charles Griffin, 1978.

    Google Scholar 

  33. Brandt JT, Triplett DA, Fair DS Characterization and comparison of immune-depleted and hereditary factor VII-deficient plasmas as substrate plasma for factor VII assays. Am J Clin Pathol 1986; 85: 583–9.

    PubMed  CAS  Google Scholar 

  34. Takase T, Tuddenham EG, Chand S, Goodall AH Monoclonal antibodies to human factor VII: production ofimmunodepleted plasma for VII:C assays. J Clin Patho11988; 41: 342-5.

    Google Scholar 

  35. Triplett DA, Brandt JT, Batard MA, Dixon JL, Fair DS Hereditary factor VII deficiency, heterogeneity defined by combined functional and immunochemical analysis. Blood 1985; 66: 1284–7.

    PubMed  CAS  Google Scholar 

  36. Bertina RM, Orlando M, Tiedemann-Aderkamp GH Preparation of a human factor VII deficient plasma. Thromb Res 1978; 13: 537–41.

    Article  PubMed  CAS  Google Scholar 

  37. Mustard JF, Secord D, Hoeksema TD, Downie HG, Rowsell HC Canine factor VII deficiency. Br J Haematol 1962; 8: 4347–54.

    Google Scholar 

  38. Gamer R, Conning DM The assay of human factor VII by means of modified factor VII deficient dog plasma. Br J Haematol 1970; 18: 57–66.

    Article  Google Scholar 

  39. Poller L, Thomson JM, Sear CHJ, Thomas W Identification of a congenital defect of factor VII in a colony of beagle dogs: The clinical use of the plasma. J Clin Pathol 1971; 24: 626–31.

    Article  PubMed  CAS  Google Scholar 

  40. Duckert F Analytische Methoden. In: Koller F, Duckert F, eds. Thrombose und Embolie. Stuttgart: Schattauer, 1983; 761–78.

    Google Scholar 

  41. Thomson JM, Poller L Factor VIII assay proficiency assessment: experience in the UK. Scand J Haematol 1984; 33 (Suppl. 41): 101–8.

    Article  Google Scholar 

  42. Maas RL, Triplett DA, Tholen D Factor VIII assay proficiency assessment: experience in the USA. Scand J Haematol 1984; 33 (Suppl. 41); 109–20.

    Google Scholar 

  43. Thompson SG, Duckert F, Haverkate F, Thomson JM The measurement of haemostatic factors in the European laboratories: quality assessment for the multicentre ECAT Angina pectoris study. Thromb Haemostas 1989; 61: 301–6.

    CAS  Google Scholar 

  44. Seligsohn U, Osterud B, Rapaport SL Coupled amidolytic assay for factor VII: its use with a clotting assay to determine the activity state of factor VII. Blood 1978; 52: 978–88.

    PubMed  CAS  Google Scholar 

  45. Boyer C, Wolf M, Rothschild C, Migand M, Arniral J, Mannucci P.M, Meyer D, Larrieu MJ An enzyme immunoassay (ELISA) for the quantitation of human factor VII. Thromb Haemostas 1986; 56: 250–5.

    CAS  Google Scholar 

  46. Takase T, Tuddenham EG, Chand S, Goodall AH Monoclonal antibodies to human factor VII: a one step immunoradiometric assay for VII: Ag. J Clin Pathol 1988; 41: 337–41.

    Article  PubMed  CAS  Google Scholar 

  47. Tirindelli MC, Mariani G, Mazzucconi MG, Iacopino G, Carbonaro M, Ghirardini A, Motta M, Mandelli F Evaluation of factor VII antigen in factor VII congenital deficiencies with a new ELISA assay. Am J Hematol 1987; 26: 313–21.

    Article  PubMed  CAS  Google Scholar 

  48. Carvalho de Sousa J, Azevedo J, Soria C, Barros F, Ribeiro C, Parreira F, Caen JP Factor VII hyperactivity in acute myocardial thrombosis. A relation to the coagulation activation. Thromb Res 1988; 51: 165–73.

    Article  PubMed  CAS  Google Scholar 

Download references

Authors
  1. G. A. Marbet
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Duckert
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Clinical Chemistry, Ribe County Hospital in Esbjerg, Esbjerg, Denmark

    Jørgen Jespersen MD DSc

  2. Haemostasis and Thrombosis Research Unit, University Hospital Leiden, Leiden, The Netherlands

    Rogier M. Bertina PhD

  3. Gaubius Laboratory, IVVO-TNO, Leiden, The Netherlands

    F. Haverkate MD

Rights and permissions

Reprints and permissions

Copyright information

© 1992 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Marbet, G.A., Duckert, F. (1992). Factor VII clotting activity. In: Jespersen, J., Bertina, R.M., Haverkate, F. (eds) ECAT Assay Procedures A Manual of Laboratory Techniques. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2992-3_7

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-2992-3_7

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5330-3

  • Online ISBN: 978-94-011-2992-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation