Abstract
DNA Restriction patterns of eighteen strains of Azospirillum were obtained with SDS-PAGE and silver staining. Each strain was shown to have a unique, stable pattern that enables its sure recognition. Strains were clustered with the UPGMA method into two main groups corresponding to the species level.
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References
Chowdhury, S. I., Hammerschmidt, W., Ludwig, H., Thein, P. and Buhk, H. J. (1986) ‘Rapid method for the identification and screening of Herpesviruses by DNA fingerprinting combined with blot hybridization’, J. Virol. Meth. 14, 285–291.
Degli-Innocenti, F., Ferdani, E., Pesenti-Barili, B., Dani, M., Giovannetti, L. and Ventura, S. (1990) ‘Identification of microbial isolates by DNA fingerprinting: analysis of ATCC Zymomonas strains’, J. Biotechnol. 13, 335–346.
Döbereiner, J. and Pedrosa, F. O. (1987) Nitrogen-fixing Bacteria in Nonleguminous Crop Plants, Science Tech Publishers, Madison.
Giovannetti, L., Ventura, S., Bazzicalupo, M., Fani, R. and Materassi, R. (1990) ‘DNA restriction fingerprint analysis of the soil bacterium Azospirillum’, J. Gen. Microbiol. 136, 1161–1166.
Krieg, N. R. and Döbereiner, J. (1984) ‘Genus Azospirillum Tarrand Krieg and Döbereiner 1979, 79AL (effective publication: Tarrand, Krieg and Döbereiner 1978, 978)’, in N. R. Krieg and J. G. Holt (eds.), Bergey’s Manual of Systematic Bacteriology, Willliams and Wilkins, Baltimore, vol. 1, pp. 94–104.
Okon, Y. (1985) ‘Azospirillum as a potential inoculant for agriculture’, Trends Biotechnol. 3, 223–229.
Sneath, P. H. A. and Sokal, R. R. (1973) Numerical Taxonomy. The Principles and Practices of Numerical Classification, W. H. Freeman, San Francisco.
Sorensen, B., Falk, E. S., Wisloff-Nilsen, E., Bjorvatn, B. and Kristiansen, B.E. (1985) ‘Multivariate analysis of Neisseria DNA restriction endonuclease patterns’, J. Gen. Microbiol. 131, 3099–3104.
Tarrand, J. J., Krieg, N. R. and Döbereiner, J. (1978) ‘A taxonomic study of the Spirillun lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov.’, Can. J. Microbiol. 24, 967–980.
Trüper, H. G. and Krämer, J. (1981) ‘Principles of characterization and identification of prokaryotes’, in M. P. Starr, H. Stolp, H. G. Triiper, A. Balows and H. G. Schlegel (eds.), The Prokaryotes, Springer-Verlag, Berlin, Heidelberg, New York, pp. 176–193.
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© 1991 Springer Science+Business Media Dordrecht
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Ventura, S., Giovannetti, L., Gori, A., Maruca, S., Montaini, P. (1991). DNA Restriction Fingerprint for the Identification of Azospirilla. In: Polsinelli, M., Materassi, R., Vincenzini, M. (eds) Nitrogen Fixation. Developments in Plant and Soil Sciences, vol 48. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3486-6_30
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DOI: https://doi.org/10.1007/978-94-011-3486-6_30
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