Synopsis
Female-specific serum proteins (FSSPs) in white-edged rockfish, Sebastes taczanowskii,were identified and partially characterized by immunochemical procedures. Two FSSPs, which clearly reacted with antiserum against egg proteins, were confirmed in the serum of mature females, and estrogen treatment induced similar FSSPs in the serum of mature males. Hence, the FSSPs were considered to be vitellogenin. The vitellogenin concentration in female fish was high during the vitellogenic period and low during gestation, parturition and the recovery period, indicating that vitellogenin is used only for yolk formation in the oocytes and not as a direct nutritional source for developing embryos during gestation. On the other hand, an FSSP (FS3), which was considered not to be vitellogenin, was also identified in the sera of mature females and males after estradiol-17β administration by using an antiserum (a-FS3) that removed the components of the male serum and egg extracts from the anti-mature female serum antiserum. Moreover, immunohistochemical observation with a-FS3 illustrated that FS3 was a major constituent of the ovarian fluid but not of vitellogenic oocytes. The cross-reactivities of these FSSPs among seven viviparous rockfishes demonstrated that vitellogenin existed in the sera of all rockfishes studied belonging to the genera Sebastes and Sebastiscus,whereas FS3 was not present in several species of Sebastes.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Aida, K., P.V. Ngan & T. Hibiya. 1973. Physiological studies on gonadal maturation of fishes-I. Sexual difference in composition of plasma protein of ayu in relation to gonadal maturation.Nippon Suisan Gakkaishi 31: 1091–1106.
deVlaming, V. 1983. Oocyte development patterns and hormonal involvements among teleosts. pp. 176–199. In J.C. Pankin, T.J. Pitcher & R.T. Duggan (ed.) Control Processes in Fish Physiology, Croom Helm, London.
deVlaming, V., D. Baltz, S. Anderson, R. Fitzgerald, G. Delahunty & M. Barkley. 1983. Aspects of embryo nutrition and excretion among viviparous embiotocid teleosts: potential endocrine involvements. Comp. Biochem. Physiol. 76A: 189–198.
Hamazaki, T., I. Iuchi & K. Yamagami. 1984. Chorion glycoprotein-like immunoreactivity in some tissues of adult female medaka. Zool. Sci. 1: 148–150.
Hamazaki, T., I. Iuchi & K. Yamagami. 1985. A spawning female-specific substance reactive to anti-chorion (egg envelope) glycoprotein antibody in the teleost, Oryzias latipes. J. Exp. Zool. 235: 269–279.
Hamazaki, T., I. Iuchi & K. Yamagami. 1987a. Production of a ‘spawning female-specific substance’ in hepatic cells and its accumulation in the ascites of the estrogen-treated adult fish, Oryzias latipes. J. Exp. Zool. 242: 325–332.
Hamazaki, T., I. Iuchi & K. Yamagami. 1987b. Purification and identification of vitellogenin and its immunohistochemical detection in growing oocytes of the teleost, Oryzias latipes. J. Exp. Zool. 242: 333–342.
Hara, A. & H. Hirai. 1978. Comparative studies on immunochemical properties of female-specific serum protein and egg yolk proteins in rainbow trout (Salmo gairdneri). Comp. Biochem. Physiol. 59B: 339–343.
Hara, A., T. Matsubara, M. Saneyoshi & K. Takano. 1984. Vitellogenin and its derivatives in egg yolk proteins of whitespotted char (Salvelinus leucomaenis). Bull. Fac. Fish. Hokkaido.Univ. 35: 144–153. (in Japanese).
Hara, A., K. Takano & H. Hirai. 1983. Immunochemical identification of female-specific serum protein, vitellogenin, in the medaka, Oryzias latipes (teleosts). Comp. Biochem. Physiol. 76A: 135–141.
Hara, A., A. Takemura, T. Matsubara & K. Takano. 1986. Immunochemical identification of female-specific serum proteins in a viviparous fish, the white-edged rockfish (Sebastes taczanowskii), during vitellogenesis and pregnancy, and after estrogen treatment. Bull. Fac. Fish. Hokkaido Univ. 37: 101–110. (in Japanese).
Hara, A., K. Yamauchi & H. Hirai. 1980. Studies on femalespecific serum protein (vitellogenin) and egg yolk protein in Japanese eel (Anguilla japonica). Comp. Biochem. Physiol. 65B: 315–320.
Korsgaard, B. 1982. Changes in activity levels of glutamate dehydrogenase and two transaminases of maternal liver during reproduction and after administration of oestradiol and progesterone in Zoarces viviparus (L.). J. Fish Biol. 20: 445–454.
Korsgaard, B. 1983. The chemical composition of follicular and ovarian fluids of the pregnant blenny (Zoarces viviparus (L.)). Can. J. Zool. 61: 1101–1108.
Korsgaard, B. & I. Petersen. 1979. Vitellogenin, lipid and carbohydrate metabolism during vitellogenesis and pregnancy, and after hormonal induction in the blenny Zoarces viviparus (L.). Comp. Biochem. Physiol. 63B: 245–251.
Le Menn, F. 1979. Some aspects of vitellogenesis in a teleostean fish: Gobius niger L. Comp. Biochem. Physiol. 62A: 495–500.
Mancini, G., A.O. Carbonara & J.F. Heremans. 1965. Immunochemical quantification of antigens by single radial immunodiffusion.Immunochemistry 2: 235–254.
Markert, J.R. & W.E. Vanstone. 1971. Egg proteins of coho salmon (Oncorhynchus kisutch): chromatographic separation and molecular weights of the major proteins in the high density fraction and their presence in salmon plasma. J. Fish. Res. Board Can. 28: 1853–1856.
Nunomura, W., A. Hara, K. Takano & H. Hirai. 1984. Immunohistochemical localization of vitellogenin in hepatic cells of some salmonid fishes. Bull. Fac. Fish. Hokkaido Univ. 34: 79–87. (in Japanese).
Ouchterlony, O. 1953. Antigen-antibody reactions in gels. IV. Types of reactions in coordinated systems of diffusion. Acta Path. Microbiol. Scand. 32: 231–240.
Plack, P.A., D.J. Pritchard & N.M. Fraser. 1971. Egg proteins in cod serum: natural occurrence and induction by injections of oestradiol 3-benzoate. Biochem. J. 121: 847–856.
Takemura, A., K. Takano & H. Takahashi. 1987. Reproductive cycle of a viviparous fish, the white-edged rockfish, Sebastes taczanowskii. Bull. Fac. Fish. Hokkaido Univ. 38: 111–125.
Teranishi, T., A. Hara & H. Takahashi. 1981. Changes of serum vitellogenin levels during the course of annual reproductive cycle of the loach, Misgurnus anguillicaudatus. Bull. Fac. Fish. Hokkaido Univ. 32: 281–292. (in Japanese).
Wallace, R. A. & K. Selman. 1981. Cellular and dynamic aspects of oocyte growth in teleosts. Amer. Zool. 21: 325–343.
Wourms, J.P. 1981. Viviparity: the maternal-fetal relationships in fishes. Amer. Zool. 21: 473–515.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Takemura, A., Hara, A., Takano, K. (1991). Immunochemical identification and partial characterization of female-specific serum proteins in white-edged rockfish, Sebastes taczanowskii . In: Boehlert, G.W., Yamada, J. (eds) Rockfishes of the genus Sebastes: Their reproduction and early life history. Developments in environmental biology of fishes, vol 11. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3792-8_6
Download citation
DOI: https://doi.org/10.1007/978-94-011-3792-8_6
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5688-5
Online ISBN: 978-94-011-3792-8
eBook Packages: Springer Book Archive