Abstract
When mammalian cells are seeded out in tissue culture they will drift down to the surface and firmly attach. The cells attach to a preadsorbed protein layers and not to the naked surface. Normally the protein layer will be a mixture of protein obtained from serum added to the tissue culture medium, but it is also possible to preadsorb pure protein layers onto the surface. This talk will discuss the many factors that affect the interaction of cells with surfaces including the time course of attachment and spreading and the forces that bind the cells to the protein layers.
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© 2000 Springer Science+Business Media Dordrecht
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Giaever, I., Keese, C.R. (2000). Attachment and Spreading of Mammalian Cells in Vitro. In: Skjeltorp, A.T., Edwards, S.F. (eds) Soft Condensed Matter: Configurations, Dynamics and Functionality. NATO Science Series, vol 552. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4189-5_7
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DOI: https://doi.org/10.1007/978-94-011-4189-5_7
Publisher Name: Springer, Dordrecht
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