Skip to main content
SpringerLink
Log in

Applications of Mass Spectrometry in Biochemical Studies of Nucleosides, Nucleotides and Nucleic Acids

  • Chapter
Selected Topics in Mass Spectrometry in the Biomolecular Sciences

Part of the book series: NATO ASI Series ((ASIC,volume 504))

Abstract

The advent of soft ionization procedures such as fast-atom bombardment, electrospray and thermospray, and, more recently, matrix-assisted laser desorption - time of flight, has greatly extended the utilization of mass spectrometry in analyses of nucleobases, nucleosides, nucleotides and nucleic acids. Here specific examples of the application of MS techniques to studies of nucleic acid base composition, sequence and modifications, of nucleoside and nucleotide antibiotic activity, and of nucleotide cell signalling functions are reviewed and potential future advances discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. S. Hanessian, D.C. DeJongh & J.A. McCloskey, Biochim. Biophys. Acta 117, 480 (1966).

    Article  CAS  Google Scholar 

  2. K. Bieman, S. Tsunakawa, J. Sannen, H. Feldmann, D. Dütting & H.G. Zachau, Agnew. Chem. Int. Ed. Engel. 5, 560 (1966).

    Google Scholar 

  3. A.M. Lawson, R.N. Stillwell, M.M. Tacker, K. Tsuboyama & J.A. McCloskey, J. Amer. Chem. Soc. 93, 1014 (1971).

    Article  CAS  Google Scholar 

  4. Q.M. Porter, Mass Spectrometry of Hererocyclic Compounds, Wiley, New York (1985).

    Google Scholar 

  5. C. Hignite, in G.R. Waller (ed) Biochemical Applications of Mass Spectrometry, Wiley, New York pp 527–566 (1979).

    Google Scholar 

  6. P.B. Farmer, Biomed. Environ. Mass Spectrom. 17, 143 (1988).

    Article  CAS  Google Scholar 

  7. C.H. Hocart & J.P. Schlunegger, Rapid Commun. Mass Spectrom. 3, 143 (1989)

    Google Scholar 

  8. C.C. Nelson & J.A. McCloskey, J. Amer. Soc. Mass Spectrom. 5, 339 (1994).

    Article  CAS  Google Scholar 

  9. C.C. Nelson & J.A. McCloskey, J. Amer. Chem. Soc. 114, 3661 (1992).

    Article  CAS  Google Scholar 

  10. E. Borck, B.S. Baliga & C.W. Gehrke, Cancer Res. 37. 3362 (1977).

    Google Scholar 

  11. T.D. McClure, K.H. Schram, K. Nakano & T. Yasaka, Nucleosides & Nucleotides 8, 1399 (1989).

    Article  CAS  Google Scholar 

  12. J.I. Langridge, T.D. McClure, S. El-Shakawi, A. Fielding, K.H. Schram & R.P. Newton, Rapid Commun. Mass Spectom. 7, 427 (1993).

    Article  CAS  Google Scholar 

  13. J.I. Langridge, T.D. McClure, K.H. Schram. S. El-Shakawi, A. Fielding & R.P. Newton, in E. Constantin (ed) Therapeutic Aspects and Analytical Methods in Cancer Research, Amudes, Strasbourg, pp 96–117 (1994).

    Google Scholar 

  14. A. McCloskey, Methods in Enzymol. 193, 825 (1990).

    Article  CAS  Google Scholar 

  15. H. Pang, K.H. Schram, D.L. Smith, S.P. Gupta, L.B. Townsend & J.A. McCloskey, J. Org. Chem. 47, 3923 (1982).

    Article  CAS  Google Scholar 

  16. P.A. Limbach, P.F. Crain & J.A. McCloskey, Nucleic Acids Res. 22, 2183 (1994).

    Article  CAS  Google Scholar 

  17. P.F. Crain & J.A. McCloskey, Nucleic Acids Res. 24, 98 (1996).

    Article  CAS  Google Scholar 

  18. I.M. Grgson, P.F. Crain, EG. Edmonds, R. Gupta, T. Hashizume, D.W. Phillipson & J.A.McCloskey, J. Biol. Chem. 268, 10076 (1993).

    Google Scholar 

  19. M.W. Kilpatrick & R.T. Waller, Zentrabl. Bakteriol. Mikrobiol. Hyg. I Abt Orig. C. 3, 79 (1982).

    CAS  Google Scholar 

  20. M. Ubukutata, K. Kimura. K. Isono, C. Nelson, J.M. Gregson & I.A. McCloskey, J. Org. Chem. 57. 6392 (1992).

    Article  Google Scholar 

  21. E. Prinsen, P. Redig, W. Van Dongen, E.L. Esmans & H. Van Onckelen, Rapid Commun. Mass Spectrom. 9, 948 (1995).

    Article  CAS  Google Scholar 

  22. H.K. Jajoo, S.M. Bennett & D.M. Kornhauser, J. Chromatog. 577, 299 (1992).

    Article  CAS  Google Scholar 

  23. D.L. Slowikowski & K.H. Schram. Nucleosides & Nucleotides 4, 309 (1985).

    Article  CAS  Google Scholar 

  24. R.P. Newton, in G. Dealty & D. Rickwood (eds) Cell Biology Labfax, Bios Scientific, Oxford. pp 153–166 (1992).

    Google Scholar 

  25. E.E. Kingston, J.H. Beynon & R.P. Newton, Biomed. Mass Spectrom. 11, 367 (1983).

    Article  Google Scholar 

  26. E.E Kingston. J.H. Beynon, R.P. Newton & J.G. Liehr, Biomed. Mars Spectrom. 12, 525 (1985).

    Article  CAS  Google Scholar 

  27. R.P. Newton, N. Gibbs, C.D. Moyse, J.L. Wiebers & E.G. Brown, P hytochem. 19. 1909 (1980).

    Article  CAS  Google Scholar 

  28. R.P. Newton, E.E. Kingston, D.E. Evans, L.M. Younis & E.G. Brown, Phytochem. 23, 1367 1986.

    Article  Google Scholar 

  29. R.P. Newton, S.G. Salih, B.J. Salvage & EE. Kingston, Biochem. J. 221, 665 (1984).

    CAS  Google Scholar 

  30. R.P. Newton, E.E. Kingston, N.A. Hakeem, S.G. Salih, J.H. Beynon & C.D. Moyse, Biochem. J. 236431 (1986).

    CAS  Google Scholar 

  31. R.P. Newton, D. Chiatante, D. Ghosh, A.G. Brenton, T.J. Walton, F.M. Hams & E.G. Brown, Phytochem. 28. 2243 (1989).

    Article  CAS  Google Scholar 

  32. R.P. Newton, T.J. Walton, S.A. Basaif, A.M. Jenkins, A.G. Brenton, D. Ghosh & F.M. Harris, Org Mass Spectrom. 24. 679 (1989).

    Article  CAS  Google Scholar 

  33. R.P. Newton, A.M. Evans, H.G. Hassan, F.M. Harris, A.G. Brenton & T.J. Walton, Org. Mass Sprctrom. 28, 899 (1993).

    Article  CAS  Google Scholar 

  34. R.P. Newton, T.I. Walton, A.G. Brenton, E.E. Kingston & F.M. Harris, Rapid Commun. Mass Spectrom. 3, 178 (1989).

    Article  CAS  Google Scholar 

  35. R.P. Newton, J.A. Khan, D. Ghosh, J.I. Langridge, A.G. Brenton, F.M. Harris & T.J. Walton, Org. Mass Spectrom. 26, 447 (1991).

    Article  CAS  Google Scholar 

  36. R.P. Newton, J.A. Khan, A.G. Brenton, J.I. Langridge, F.M. Harris & T.J. Walton, Rapid Commun. Mass Spectrom. 6, 601 (1992).

    Article  CAS  Google Scholar 

  37. R.P. Newton, A.M. Evans, J.I. Langridge, T.J. Walton, F.M. Harris & A.G. Brenton, Analyt. Biochem. 224, 32 (1995).

    Article  CAS  Google Scholar 

  38. T.J. Walton, D. Ghosh, R.P. Newton, A.G. Brenton & F.M. Harris, Nucleosides & Nucleotides 9, 365 (1990).

    Article  Google Scholar 

  39. D. Ghosh, R.P. Newton, A.G. Brenton, F.M. Harris, M.P. Donovan, E.G. Brown & T.J. Walton, Analyt. Chimica Acta 247, 187 (1991).

    Article  CAS  Google Scholar 

  40. R.P. Newton, M.A. Bayliss, A.C.R. Wilkins, D.E. Games, A.G. Brenton, J.I. Langridge, T.J. Walton & F.M. Harris, J. Mass Spectrom. & Rapid Commun. Mass Spectrom.S107 (1995).

    Google Scholar 

  41. R.P. Newton, E.E. Kingston & A. Overton, Rapid Commun. Mass Spectrom. 9, 305 (1995).

    Article  CAS  Google Scholar 

  42. R.P. Newton, N.A. Hakeem, B.J. Salvage, G. Wassenaar & E.E. Kingston, Rapid Commun. Mass Spectrom. 2, 118 (1988).

    Article  CAS  Google Scholar 

  43. K.H. Schram, Methods in Enzymol. 193, 791 (1990).

    Article  CAS  Google Scholar 

  44. J.I. Langridge, A.M. Evans, D. Ghosh, T.J. Walton, A.G. Brenton, F.M. Harris & R.P. Newton, Analyt. Chimica Acta 247, 177 (1991).

    Article  CAS  Google Scholar 

  45. J.I. Langridge, A.G. Brenton, T.J. Walton, F.M. Harris & R.P. Newton, Rapid Commun. Mass Spectrom. 7, 293 (1993).

    Article  CAS  Google Scholar 

  46. E. Witters, L. Roef, R.P. Newton, W. Van Dongen, E.L. Esmans & H. Van Onckelen, Rapid Commun. Mass Spectrom. 10, 225 (1996).

    Article  CAS  Google Scholar 

  47. D.R. Phillips, M. Uramoto, K. Isono & J.A.McCloskey, J. Org. Chem. 58, 854 (1993).

    Article  CAS  Google Scholar 

  48. S.C. Pomerantz, J.A. Kowalak & J.A. McCloskey, J. Amer. Chem. Soc. 4, 204 (1993).

    CAS  Google Scholar 

  49. P.A. Limbach, P.F. Crain & J.A. McCloskey, J. Amer. Soc. Mass Spectrom. 6, 19 (1995).

    Article  Google Scholar 

  50. T.R. Covey, R.F. Bonner, B.I. Shushan & J. Hellion, Rapid Commun. Mass Spectrom. 2, 249 (1988).

    Article  CAS  Google Scholar 

  51. L. Grothajn, H. Blocher & R. Frank, Biomed. Mass Spectrom. 12, 514 (1985).

    Article  Google Scholar 

  52. J.T. Stults & J.C. Masters, Rapid Commun. Mass Spectrom. 5, 359 (1991).

    Article  CAS  Google Scholar 

  53. J.A. Kowalak, S.C. Pomerantz, P.F. Crain & J.A. McCloskey, Nucleic Acids Res. 21, 4577 (1993).

    Article  CAS  Google Scholar 

  54. J.A. Kowalak, E. Bruenger, & J.A. McCloskey, J. Biol. Chem. 270, 17758 (1995).

    Article  CAS  Google Scholar 

  55. J.A. Kowalak, E. Bruenger, T. Hashizume, J.M. Peltier, J. Ofengand & J.A. McCloskey, Nucleic Acids Res. 24, 688 (1996).

    Article  CAS  Google Scholar 

  56. H.F. Noller, J. Bacteriol. 175, 5297 (1993).

    CAS  Google Scholar 

  57. E. Nordhoff, A. Igendoh, R. Cramer, A. Overburg, B. Stahl, M. Karas, F. Hillencamp & P.F. Crain, Rapid Commun. Mass Spectrom. 6, 771 (1992).

    Article  CAS  Google Scholar 

  58. E. Nordhoff, F. Kirpekar, S. Hahner, F. Hillencamp & P. Roepstorff, in R.P. Newton & T.J. Walton (eds) Applications of Mass Spectrometry in Plant Science Research, Oxford University Press, Oxford, pp 86–101 (1996).

    Google Scholar 

  59. E. Nordhoff, F. Kirpekar, M. Karas, R. Cramer, S. Hairnet., F. Hillencamp, K. Kristiansen, P. Roepstorff & A. Lezius, Nucleic Acids Res. 22, 2460 (1994).

    Article  CAS  Google Scholar 

  60. E. Nordhoff, R. Cramer, M. Karas, F. Hillencamp, F. Kirpekar, K. Kristiansen & P. Roepstorff, Nucleic Acids Res. 21, 3347 (1993).

    Article  CAS  Google Scholar 

  61. K. Schneider & B.T. Chait, Proc. XXXXth ASMS Conf. Mass Spectrom. Washington, 144 (1994).

    Google Scholar 

  62. K.J. Wu, T.A. Shalter & C.H. Becker, Analyt. Chem. 66, 1637 (1994).

    Article  CAS  Google Scholar 

  63. E. Nordhoff, M. Karas, R. Cramer, S. Hahner, F. Hillencamp, F. Kirpekar, A. Lezius, J. Muth, C. Meier & J.W. Engels, J. Mass Spectrom. 30, 99 (1995).

    Article  CAS  Google Scholar 

  64. M.C. Fitzgerald, L. Zhu & L.M. Smith, Rapid Commun. Mass Spectrom. 7, 895 (1993).

    Article  CAS  Google Scholar 

  65. M.C. Fitzgerald & L.M. Smith, Annu. Rev. Biophys. Biomol. Struct. 24, 117 (1995).

    Article  CAS  Google Scholar 

  66. U. Pieles, W. Zurcher, M. Schär & H.E. Moser, Nucleic Acids Res. 21, 3191 (1993).

    Article  CAS  Google Scholar 

  67. K. Schneider & B.T. Chait, Nucleic Acids Res. 23, 1570 (1995).

    Article  CAS  Google Scholar 

  68. F. Kirpekar, E. Nordhoff, K. Kristiansen, P. Roepstorff, S. Hahner & F. Hillencamp, Rapid Commun. Mass Spectrom. 9, 525 (1995).

    Article  CAS  Google Scholar 

  69. S.A. McLuckey, G.J. van Berkel & G.L. Glish, J. Amer. Mass Spectrom. 3, 60 (1992).

    Article  CAS  Google Scholar 

  70. M.J. Doktycz, S. Habibi-Gondarzi & S.A. McLuckey, Analyt. Chem. 66, 3416 (1994).

    Article  CAS  Google Scholar 

  71. S.A. McLuckey & S. Habibi-Gondarzi, J. Amer. Chem. Soc. 5, 740 (1993).

    Google Scholar 

  72. E. Gentil & J. Banoub, J. Mass Spectrom. 31, 83 (1996).

    Article  CAS  Google Scholar 

  73. J.A. McCloskey & P.F. Crain, Int. J. Mass Spectrom. Ion Processes 118, 593 (1992).

    Article  Google Scholar 

  74. M.M Manson, Brit. J. Ind. Med. 37, 317 (1980).

    CAS  Google Scholar 

  75. E.G. Van den Eeckhout, A. De Bmyn, H. Pepermans, E.L. Esmans, I. Vryens, J. Claereboudt, M. Claeys & J.E. Sinsheimer, J. Chromatog. 504, 113 (1990).

    Article  Google Scholar 

  76. E.G. Van den Eeckhout, J. Coene, J. Claereboudt, F. Borremans, M. Claeys, E.L. Esmans & J.E. Sinsheimer, J. Chromatog. 514, 317 (1991).

    Google Scholar 

  77. J. Claereboudt, E.L. Esmans, E.G. Van den Eeckhout & M. Claeys, Nucleosides & Nucleotides 9, 333 (1990).

    Article  CAS  Google Scholar 

  78. F. Lemière, E.L. Esmans, W. Van Dongen, E. Van den Eeckhout & H. Van Onckelen, J. Chromatog. 647, 211 (1993).

    Article  Google Scholar 

  79. K. Vanhoutte, P. Joos, F. Lemière, W. Van Dongen, E.L. Esmans, M. Claeys & E. Van den Eeckhout, J. Mass Spectrom. 30, 1453 (1995).

    Article  CAS  Google Scholar 

  80. K. Vanhoutte, P. Joos, F. Lemière, W. Van Dongen, E.L. Esmans & E. Van den Eeckhout, J. Mass Spectrom. & Rapid Commun. Mass Spectrom.S143 (1995).

    Google Scholar 

  81. O.A. Boryak, M.V. Kosevich, V.S. Shelkovsky and Y.P. Blagoy, Rapid Commun. Mass Spectrom. 10, 197 (1996).

    Article  CAS  Google Scholar 

  82. K. O’Donaghue, T.A. Brown, J.F. Carter & R.P. Evershed, Rapid Commun. Mass Spectrom. 8, 503 (1994).

    Article  Google Scholar 

  83. M.K. Hellerstein & R.A. Neese, Amer. J. Physiol. 263, E988 (1992).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biochemistry Group, School of Biological Sciences, University of Wales Swansea, Singleton Park, Swansea, SA2 8PP, UK

    Russell P. Newton

Authors
  1. Russell P. Newton
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Medical School, University of Texas, Houston, Texas, USA

    Richard M. Caprioli

  2. SESMA-C.N.R., Napoli, Italy

    Antonio Malorni

  3. Dipartimento di Chimica, Arcavacata di Rende, Università della Calabria, Italy

    Giovanni Sindona

Rights and permissions

Reprints and permissions

Copyright information

© 1997 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Newton, R.P. (1997). Applications of Mass Spectrometry in Biochemical Studies of Nucleosides, Nucleotides and Nucleic Acids. In: Caprioli, R.M., Malorni, A., Sindona, G. (eds) Selected Topics in Mass Spectrometry in the Biomolecular Sciences. NATO ASI Series, vol 504. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5165-8_22

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-5165-8_22

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6171-1

  • Online ISBN: 978-94-011-5165-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation