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The Oxidative Burst System in Plants: A Strategic Signal Transduction System for Triggering Active Defense and for Parasites to Overcome

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Molecular Genetics of Host-Specific Toxins in Plant Disease

Part of the book series: Developments in Plant Pathology ((DIPP,volume 13))

Abstract

The oxidative burst, a rapid Superoxide generation (OXB), was demonstrated in intact and wounded tissues, suspension cultured cells, protoplasts and isolated plasma membrane vesicles of Solanaceae plants at a very early stage after infection with incompatible but not compatible, Phytophthora spp. or treatment with their hyphal wall (HWC) elicitors. The OXB was based on a rapid activation of O2 - generating NAD(P)H oxidase in plasma membrane at the beginning of induced resistance. This enzyme system may be activated through a primary step of signal transduction involving Ca2+ influx through Ca2+ channels, calmodulin, protein kinase, and GTP binding protein.

Infection with compatible Phytophthora species caused no or little OXB or other defense-related responses, regardless of whether they expressed the HWC-elicitors. P. infestons released water soluble glucans during cystospore-germination and in the process of formation of infection vesicles through the host cell wall. Only glucans derived from compatible races suppressed the HWC or infection vesicle elicitor-induced OXB and hypersensitive cell death in sliced tissues, suspension cell cultures and protoplasts. The suppression of OXB was evident only when protoplasts were treated with glucans 10 sec before, but not after, elicitation by infection vesicles.

These observations form the basis of a concept that the OXB system may be a strategic signal transduction system for induction of active defense in Solanaceae plants as well as for Phytophthora spp. to overcome in successful parasitism.

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Authors and Affiliations

  1. Plant Pathology Field, Division of Bioresources and Functions, Department of Biological Mechanism and Function Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-Ku, Nagoya, 464-01, Japan

    N. Doke, L. M. Sanchez, H. Yoshioka, K. Kawakita, Y. Miura & H.-J. Park

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  1. N. Doke
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  2. L. M. Sanchez
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  3. H. Yoshioka
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  4. K. Kawakita
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  5. Y. Miura
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  6. H.-J. Park
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Editors and Affiliations

  1. Faculty of Agriculture, Tottori University, Koyama, Tottori, Japan

    Keisuke Kohmoto

  2. Department of Plant Pathology, Cornell University, Ithaca, New York, USA

    Olen C. Yoder

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© 1998 Springer Science+Business Media Dordrecht

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Doke, N., Sanchez, L.M., Yoshioka, H., Kawakita, K., Miura, Y., Park, HJ. (1998). The Oxidative Burst System in Plants: A Strategic Signal Transduction System for Triggering Active Defense and for Parasites to Overcome. In: Kohmoto, K., Yoder, O.C. (eds) Molecular Genetics of Host-Specific Toxins in Plant Disease. Developments in Plant Pathology, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5218-1_37

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  • DOI: https://doi.org/10.1007/978-94-011-5218-1_37

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6197-1

  • Online ISBN: 978-94-011-5218-1

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