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Carbon nutrition in vitro — regulation and manipulation of carbon assimilation in micropropagated systems

  • Chapter
Automation and environmental control in plant tissue culture

Abstract

Micropropagation is one of the first biotechnologies to be extensively used by industry. This technique allows efficient and rapid multiplication of many fruit, vegetable and ornamental species and is now widely used in the trade. However, despite its extraordinary potential, it is still confronted with many problems. The stimulation of natural propagation capacity, which is desirable in terms of production efficiency, induces several anatomical, morphological and physiological changes that affect the growth of plantlets very seriously upon transfer to ex vitro conditions. A complete understanding of the conditions leading to the appearance of an aberrant morphology and physiology in vitro must be achieved in order to optimize growth of the plantlets and their survival upon transfer to acclimatization. This knowledge is necessary to increase the profitability of tissue culture laboratories and to extend the technique to industrial, agronomic and forestry crops.

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Abbreviations

IRGA:

Infrared gas analyzer

Kcat:

catabolic site turnover rate

PEPC:

Phosphoenolpyruvate carboxylase

PGA:

Phosphoglyceraldehyde

Pi:

Inorganic orthophosphate

Rubisco:

Ribulose-1, 5-bisphosphate carboxylase/ oxygenase

RuBP:

Ribulose-1, 5-bisphosphate

SPS:

Sucrose Phosphate Synthase

TCA:

Tricarboxylic acid cycle

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Authors and Affiliations

  1. Horticultural Research Center, Department of Plant Science, Faculty of Agriculture and Food, Laval University, Québec, G1K 7P4, Canada

    Yves Desjardins & Chafik Hdider

  2. Laboratory of Horticulture, Rijksuniversiteit Gent, Coupure Links 653, B-9000, Gent, Belgium

    Jan de Riek

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  1. Yves Desjardins
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  2. Chafik Hdider
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  3. Jan de Riek
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  1. Forest Biotechnology Centre, Carter Holt Harvey Forestry Group, Rotorua, New Zealand

    Jenny Aitken-Christie

  2. Faculty of Horticulture, Chiba University, Matsudo, Chiba, Japan

    Toyoki Kozai

  3. Plant Sciences Lab, University of Illinois, Urbana, Illinois, USA

    Mary Ann Lila Smith

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Desjardins, Y., Hdider, C., de Riek, J. (1995). Carbon nutrition in vitro — regulation and manipulation of carbon assimilation in micropropagated systems. In: Aitken-Christie, J., Kozai, T., Smith, M.A.L. (eds) Automation and environmental control in plant tissue culture. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-8461-6_18

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  • DOI: https://doi.org/10.1007/978-94-015-8461-6_18

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