Dialysis Perfusion Culture of Animal Cells for the Production of Biologicals

Yamaji, H.; Amos, B.; Emery, A. N.; Al-Rubeai, M.

doi:10.1007/978-94-017-0728-2_21

H. Yamaji²,
B. Amos²,
A. N. Emery² &
…
M. Al-Rubeai²

Part of the book series: Animal Cell Technology: Basic & Applied Aspects ((ANICELLTECH,volume 12))

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Abstract

Recombinant CHO and NS0 cells were grown in a stirred laboratory bioreactor equipped with an integral tubular dialysis membrane with either 12 or 100 kDa molecular weight cut-off. The use of the dialysis membrane, which allowed perfusion of nutrients through its pores, enabled considerable increases in cell and product concentrations over those obtained in batch culture, with substantial improvements in growth and product yields on serum.

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References

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Authors and Affiliations

School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
H. Yamaji, B. Amos, A. N. Emery & M. Al-Rubeai

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H. Yamaji
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B. Amos
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A. N. Emery
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M. Al-Rubeai
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Editors and Affiliations

Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan
Sanetaka Shirahata , Kiichiro Teruya & Yoshinori Katakura , &

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Yamaji, H., Amos, B., Emery, A.N., Al-Rubeai, M. (2002). Dialysis Perfusion Culture of Animal Cells for the Production of Biologicals. In: Shirahata, S., Teruya, K., Katakura, Y. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 12. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0728-2_21

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DOI: https://doi.org/10.1007/978-94-017-0728-2_21
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5934-5
Online ISBN: 978-94-017-0728-2
eBook Packages: Springer Book Archive

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