Abstract
The mechanism of alkane hydroxylation by rat hepatic cytochrome P450 enzymes has been studied with hypersensitive radical probes, precursors to radicals that have lifetimes of only a few picoseconds. The results indicate that some of the previous evidence for a radical intermediate in the hydroxylation reaction arose from production of an unanticipated cationic species and that the hydroxylation occurs by a non-synchronous insertion process with a “radical” lifetime of only ca. 70 femtoseconds.
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Newcomb, M., Le Tadic-Biadatti, MH. (1997). Hypersensitive Probing for Radicals in Cytochrome P450 Hydroxylations. In: Minisci, F. (eds) Free Radicals in Biology and Environment. NATO ASI Series, vol 27. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1607-9_7
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DOI: https://doi.org/10.1007/978-94-017-1607-9_7
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