Abstract
Soil is a large sink for organic carbon within the terrestrial biosphere. Practices which cause a decline in soil organic matter cause CO2 release, in addition to damaging soil resilience and, often, agricultural productivity. The soil micro-organisms (collectively the soil microbial biomass) are the agents of transformation of soil organic matter, nutrients and of most key soil processes. Their activities are much influenced by soil physico-chemical and ecological interactions. This paper addresses two key issues. Firstly, ways of managing, and the extent to which it is possible to manage, soil biological functions. Secondly, the methodologies currently available for studying soil micro-organisms, and the functions they mediate, are discussed. It is concluded that, as the world population develops in this new millennium, there will be an increased dependence upon biological processes in soil to provide adequate crop nutrition for the majority of the world’s farmers. Although a major increase in the use of artificial fertilisers will be necessary on a global scale, this will not be an option for large numbers of farmers due to their poverty. Instead they will rely on recycling of nutrients from animal and vegetable composts and urban wastes, and biological cycling from nitrogen fixation and mycorrhizae. The challenge is to select the most appropriate topics for further research. Not all aspects are likely to lead to significantly improved agricultural productivity, or sustainability within the foreseeable future.
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References
Alef K and Nannipieri P (eds) (1995) Methods in Applied Soil Microbiology and Biochemistry. Academic Press London
Amann RI, Ludwig L and Schleifer K-H (1995) Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 59: 143–169
Anderson JM (1988) The role of soil fauna in agricultural systems. In: Wilson JR (ed) Advances in Nitrogen Cycling in Agricultural Ecosystems, pp 89–112. CAB International, Wallingford
Anderson JPE and Domsch KH (1978) A physiological method for the quantitative measurement of microbial biomass in soils. Soil Biol Biochem 10: 215–221
Balba MT (1993) Microorganisms and detoxification of industrial waste. In: Jones DG (ed) Exploitation of Microorganisms. Chapman and Hall, London
Barraclough D and Smith MJ (1987) The estimation of mineralization, immobilization and nitrification in nitrogen-15 field experiments using computer simulation. J Soil Sci 38: 519–530
Bashan Y and Holguin G (1997) Azospirillum-plant relationships: Environmental and physiological advances. Can J Microbiol 43: 103–121
Bloem J, Bolhuis PR, Veninga MR and Wieringa J (1995) Microscopic methods for counting bacteria and fungi in soil. In: Alef K and Nannipieri P (eds) Methods in Applied Soil Microbiology and Biochemistry. Academic Press, London
Borneman J, Skroch PW, O’Sully KM, Palus JA, Rumjanek NG, Jansen JL, Nienhuis J and Triplett EW (1996) Molecular microbial diversity of an agricultural soil in Wisconsin. Appl Environ Microbiol 62: 1935–1943
Bouwman LA, Bloem J, Boogert van den PHJF, Bremer F, Hoenderboom GHJ and Ruiter de PC (1994) Short-term and long-term effects on bacteriavorous nematodes and nematophagus fungi on carbon and nitrogen mineralization in microcosms. Biol Fertil Soils 17: 249–256
Braux A-S, Minet J, Tamani-Shacoori Z, Riou G and Cornier M (1997) Direct enumeration of injured Escherichia coil cells harvested onto membrane filters. J Microb Methods 31: 1–8
Brock TD (ed) (1988) Thermophiles. John Wiley, London
Brookes PC (1994) The use of microbial parameters in monitoring soil pollution by heavy metals. Biol Fertil Soils 19: 269–279
Brookes PC and McGrath SP (1984) Effects of metal toxicity on the size of the soil microbial biomass. J Soil Sci 35: 341–346
Burns, RG (ed) (1978) Soil Enzymes. Academic Press, London
Cai Y, Ohmomo S, Ogawa M and Kumai S (1997) Effect of NaC1- tolerantlactic acid bacteria and NaC1 on the fermentation characteristics and aerobic stability of silage. J Appl Microbiol 83: 307–313
Cassman KG, De Datta SK, Olk DC, Alcantara J, Samson M, Descalsota J and Dizon M (1995) Yield decline and the nitrogen economy of long-term experiments on continuous irrigated rice systems in the tropics. In: Lal R and Stewart BA (eds) Soil Management: Experimental Basis for Sustainability and Environmental Quality, pp 181–222. CRC/Lewis Publishers, Boca Raton, FL, USA
Chander K, Brookes PC and Harding SA (1995) Microbial biomass dynamics following addition of metal-enriched sewage sludges to a sandy loam. Soil Biol Biochem 27: 1409–1421
Cooper R (1959) Bacterial fertilizers in the Soviet Union. Soils Fertil 22: 327–333
Davidson EA, Stark MJ and Firestone MK (1990) Microbial production and consumption of nitrate in an annual grassland. Ecology 71: 1968–1975
Davison J (1988) Plant beneficial bacteria. Bio-Technology 6: 282–286
de Freitas JR, Banerjee MR and Germida JJ (1997) Phosphatesolubilizing rhizobacteria enhance the growth and yield but not phosphorus uptake of canola (Brassica napus L.). Biol Fertil Soils 24: 358–364
Degens BP and Harris JA (1997) Development of a physiological approach to measuring the catabolic diversity of soil microbial communities. Soil Biol Biochem 29: 1309–1320
Drury CF, Voroney RP and Beauchamp EG (1991) Availability of NH- -N to microorganisms and the soil internal N cycle. Soil Biol Biochem 23: 165–169
Elliott PW, Knight D and Anderson JM (1990) Denitrification in earthworm casts and soil under different fertilizer and drainage regimes. Soil Biol Biochem 22: 601–605
Fischer G and Heilig GK (1998) Population momentum and the demand on land and water resources. In: Greenland DJ, Gregory PJ and Nye PH (eds) Land Resources: On the Edge of the Malthusian Precipice? Philosophical Transactions of the Royal Society of London B, pp 9–29. CAB International, Wallingford, UK
Foster RC (1988) Microenvironments of soil microorganisms. Biol Fertil Soils 6: 189–203
Fritze H, Niini S, Mikkola K and Mäkinen A (1989) Soil microbial effects of a Cu-Ni smelter in south western Finland. Biol Fertil Soils 8: 87–94
Frostegârd A, Tunlid A and Bâârth E (1993) Phospholipid fatty acid composition, biomass, and activity of microbial communities from two soil types experimentally exposed to different heavy metals. Appl Environ Microbiol 59: 3605–3617
Garland JL and Mills AL (1991) Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole carbon source utilization. Appl Environ Microbiol 57: 2351–2359
Gaunt JL, Neue HU, Cassman KG, Olk DC, Arah JRM, Witt C, Ottow JCG and Grant IFG (1995) Microbial biomass and organic matter turnover in wetland rice soils. Biol Fertil Soils 19: 333342
Goulding KTG, Hütsch BW, Webster CP, Willison TW and Powlson DS (1995) The effect of agriculture on methane oxidation in soil. Philos Trans R Soc Lond A 351: 313–325
Greenland DJ (1997) Soil conditions and plant growth. Soil Use Manage 13: 169–177
Grisi B, Grace C, Brookes PC, Benedetti A and Dell’Abate MT (1998) Temperature effects on organic matter and microbial biomass dynamics in temperate and tropical soils. Soil Biol Biochem 30: 1309–1315
Harley JL and Smith SE (1983) The Mycorrhizal Symbiosis. Academic Press, London
Haron K, Brookes PC, Anderson JM and Zakaria ZZ (1998) Microbial biomass and soil organic matter dynamics in oil palm (Elaeis guineensis Jacq.) plantations, West Malaysia. Soil Biol Biochem 30: 547–552
Hassink J, Bouwman LA, Zwart KB and Brussard L (1993) Relationships between habitable pore space, soil biota and mineralization rates in grassland soils. Soil Biol Biochem 25: 47–55
Hirsch PR (1996) Population dynamics of indigenous and genetic- ally modified rhizobia in the field. New Phytol 133: 159–171
Inubushi K, Brookes PC and Jenkinson DS (1991) Soil microbial biomass C, N and ninhydrin-N in aerobic and anaerobic soils measured by the fumigation-extraction method. Soil Biol Biochem 23: 737–741
Jarvis SC, Stockdale EA, Shepherd MA and Powlson DS (1996) Nitrogen mineralisation in temperate agricultural soils: processes and measurement. Adv Agron 57: 187–235
Jenkinson DS (1966) Studies on the decomposition of plant material in soil. II. Partial sterilisation of soil and the soil biomass. J Soil Sci 17: 280–302
Jenkinson DS and Powlson DS (1976) The effects of biocidal treatments on metabolism in soil. V. A method for measuring soil biomass. Soil Biol Biochem 8: 167–177
Jenkinson DS (1984) The supply of nitrogen from the soil. In: The Nitrogen Requirement of Cereals, Reference Book No. 385, pp 79–93. ADAS, Ministry of Agriculture, Fisheries and Food, London
Jones D Gareth (ed) (1993) Exploitation of Microorganisms. Chapman and Hall, London.
Joos H, Lambert B, Leyns F, de Roek A and Swings J (1988) Inventory of natural rhizobacterial populations from different crop plants. In: Sussman M, Collins CH, Skinner FA, Stewart-Tull DE (eds) Release of Genetically-Engineered Microorganisms. Academic Press, London
Killham K (1985) A physiological determination of the impact of environmental stress on the activity of microbial biomass. Environ Poll (Series A) 38: 204–283
Kirkham D and Bartholomew WV (1954) Equations for following nutrient transformations in soil utilizing tracer data. Soil Sci Soc Am Proc 18: 33–34
Kirkham D and Bartholomew WV (1955) Equations for following nutrient transformations in soil utilizing tracer data. II. Soil Sci Soc Am Proc 19: 189–192
Kuikman PJ and van Veen JA (1989) The impact of protozoa on the availability of bacterial nitrogen to plants. Biol Fertil Soils 8: 13–18
Lal R (1998) Degradation and resilience of soils. In: Greenland DJ, Gregory PJ and Nye PH (eds) Land Resources: On the Edge of the Malthusian Precipice? Philos Trans R Soc Lond B, pp 137–150 CAB International, Wallingford, UK.
Lin Q and Brookes PC (1996) Comparison of methods to measure microbial biomass in unamended, ryegrass-amended and fumigated soils. Soil Biol Biochem 28: 933–939
Macdonald R McL (1986) Nitrification in soil: an introductory history. In: Prosser J (ed) Nitrification. IRL Press, Oxford
Marstorp H and Kirchmann H (1991) Carbon and qnitrogen mineralization and crop uptake of nitrogen from six green manure legumes decomposing in soil. Acta Agric Scand 41: 243–252
Mendum TA, Sockett RE and Hirsch PR (1999) Use of molecular and isotopic techniques to monitor the response of autotrophic ammonia-oxidizing populations of the ß subdivision of the class Proteobacteria in arable soils to nitrogen fertilizer. Appl Environ Microbiol 84: 551–558
Murphy DV, Bhogal A, Shepherd MA, Goulding KWT, Jarvis SC, Barraclough D and Gaunt JL (1999) Comparison of 15N labelling methods to measure gross nitrogen mineralisation. Soil Biol Biochem 31: 2015–2024
Myrold DD and Tiedje JM (1986) Simultaneous estimation of several nitrogen cycle rates using 15N: Theory and application. Soil Biol Biochem 18: 559–569
Nicholson PS and Hirsch PR (1998) The effects of pesticides on the diversity of culturable soil bacteria. J Appl Microbiol 84: 551–558
Ocio JA and Brookes PC (1990) An evaluation of methods for measuring the microbial biomass in soils following recent additions of wheat straw and the characterisation of the biomass that develops. Soil Biol Biochem 22: 685–694
Olk DC and Senesi N (2000) Properties of chemically extracted soil organic matter in intensively cropped lowland rice soils. In: Kirk GJD and Olk DC (eds) Olk DC and Senesi N, pp 65–87. International Rice Research Institute.
Palm C (1997) Nutrient management: Combined use of organic and inorganic fertilizers for increasing soil phosphorus availability. In: Swift MJ (ed) The Biology and Fertility of Tropical Soils, Report of the Tropical Soil Biology and Fertility Programme, pp 4–7. TSBF/UNESCO, Nairobi, Kenya
Palm CA and Sanchez PA (1991) Nitrogen release from the leaves of some tropical legumes as affected by their lignin and polyphenolic contents. Soil Biol Biochem 23: 83–88
Parker JH, Smith GA, Fredrickson HL, Vestal JR and White DC (1982) Sensitive assay, based on hydroxy fatty acids from lipopolysaccharide lipid A, for gram-negative bacteria in sediments. Appl Environ Microbiol 44: 1170–1177
Pieri C (1992) Fertility of Soils: A Future for Farming in the West African Savanna. Springer-Verlag, Berlin. Powlson DS (1994) The soil microbial biomass: Before, beyond and back. In: Ritz K, Dighton J and Giller ICE (eds) Beyond the Biomass, pp 3–20. John Wiley and Sons, Chichester
Powlson DS (1997) Integrating agricultural nutrient management with environmental objectives — current state and future prospects. Proceedings No. 402, The Fertiliser Society, 42 pp
Powlson DS and Barraclough D (1993) Mineralization and assimilation in soil-plant systems. In: Knowles R and Blackburn TH (eds) Nitrogen Isotope Techniques, pp 209–242. Academic Press Inc., California
Rahn CR, Vaidyanathan LV and Paterson CD (1992) Nitrogen residues from brassica crops. Aspects Appl Biol 30: 263–270
Rubenchik LI (1960) Azotobacter and its use in Agriculture. Translated from the Russian, Israel Program for Scientific Translations, Jerusalem
Ruz Jerez BE, Ball PR and Tillman RW (1988) Role of earthworms in N release from herbage residues. In: Jenkinson DS and Smith KA (eds) Nitrogen Efficiency in Agricultural Soils, pp 355–370. Elsevier, London
Saffigna PG, Powlson DS, Brookes PC and Thomas GA (1989) Influence of sorghum residues and tillage on soil organic matter and soil microbial biomass in an Australian vertisol. Soil Biol Biochem 21: 759–765
Sanchez PA, Buresh RI and Leakey RRB (1998) Trees, soils, and food security. In: Greenland DJ, Gregory RI and Nye PH (eds) Land Resources: On the Edge of the Malthusian Precipice? Philosophical Transactions of the Royal Society of London B, Vol. 352, pp 949–961. CAB International, Wallingford, UK
Scheu S (1987) Microbial activity and nutrient dynamics in earthworm casts (Lumbricidae). Biol Fertil Soils 5: 230–254
Smith JU, Bradbury NJ and Addiscott TM (1996) SUNDIAL: a PC-based system for simulating nitrogen dynamics in arable land. Agron J 88: 38–43
Smith P, Andrén O, Brussaard L, Dangerfield M, Ekschmitt K, Lavelle P and Tate K (1998) Soil biota and global change at the ecosystem level: describing soil biota in mathematical models. Global Change Biol 4: 773–784
Stotzky G (1997) Soils as an environment for microbial life. In: van Olsas JD, Trevors EM and Wellington H (eds) Modern Soil Microbiology, pp 1–20. Marcel Dekker Inc., New York
Torsvik V, Goksoyr J and Daae FL (1990) High diversity in DNA of soil bacteria. Appl Environ Microbiol 56: 782–787
Tunlid A and White DC (1992) Biochemical analysis of biomass, community structure, nutritional status and metabolic activity of microbial communities in soil. In: Stotzky G and Bollag J-M (eds) Soil Biochemistry Vol. 7, pp 229–262. Marcel Dekker, New York
Underhill SE (1990) Techniques for studying the microbial ecology of nitrification. Methods Microbiol 22: 417–445
Vance ED, Brookes PC and Jenkinson DS (1987) An extraction method for measuring soil microbial biomass C. Soil Biol Biochem 19: 703–707
Wedin DA and Tilman D (1990) Species effects on nitrogen cycling: A test with perennial grasses. Oecologia 84: 433–441
Willison TW, Baker JC, Murphy DV and Goulding KWT (1998) Comparison of a wet and dry 15N isotopic dilution technique as a short-term nitrification assay. Soil Biol Biochem 30: 661–663
Woese CR (1987) Bacterial evolution. Microbiol Rev 51: 221–271
Woods LE, Cole CV, Elliott ET, Anderson RV and Coleman DC (1982) Nitrogen transformations in soil as affected by bacterialmicrofaunal interactions. Soil Biol Biochem 14: 93–98
Wu J, O’Donnell AG, Syers JK, Adey MA and Vityakon P (1998) Modelling soil organic matter changes in ley-arable rotations in sandy soils of Northeast Thailand. European J Soil Sci 49: 463470
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Powlson, D.S., Hirsch, P.R., Brookes, P.C. (2001). The role of soil microorganisms in soil organic matter conservation in the tropics. In: Martius, C., Tiessen, H., Vlek, P.L.G. (eds) Managing Organic Matter in Tropical Soils: Scope and Limitations. Developments in Plant and Soil Sciences, vol 93. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2172-1_5
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DOI: https://doi.org/10.1007/978-94-017-2172-1_5
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