Abstract
Here we present a microbiology microfluidic device that permits the growth of a bacterium on-chip. Our device was fabricated from Polydimethylsiloxane using an SU-8 photoresist positive master. The device consists of a microfabricated capillary whereby a suspension of bacterial cells are flowed through and incubated. Incubation of an Escherischia coli expressing a green fluorescent protein was successfully monitored optically and by fluorescence measurements over a 4–5 hour period. The system confers several advantages in comparison to conventional bulk growth techniques such as real-time monitoring, reduction in inoculum volume and a considerable reduction of biological waste.
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© 2000 Springer Science+Business Media Dordrecht
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Monaghan, P.B., Manz, A., Nichols, W.W. (2000). Microbiology On-a-Chip. In: van den Berg, A., Olthuis, W., Bergveld, P. (eds) Micro Total Analysis Systems 2000. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2264-3_25
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DOI: https://doi.org/10.1007/978-94-017-2264-3_25
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5496-8
Online ISBN: 978-94-017-2264-3
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