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Isolation and cultivation of teleost hepatocytes

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The Hepatocyte Review

Abstract

After the original description by Berry and Friend in 1969 of the high yield isolation of rat liver parenchymal cells by collagenase perfusion [1], the use of isolated hepatocytes was soon established as an ideal system for the study of many aspects of hepatic metabolism [2]. In 1976, Birnbaum and colleagues [3] adapted the technique of hepatocyte isolation for studies of hormone-stimulated glycogenolysis in goldfish cells. Since then, isolated fish hepatocytes have been used intensively in fresh suspensions and in primary culture as a powerful, yet simple tool for the investigation of piscine hepatic functions. Cultured hepatocytes have proved to be well suited for applications not only in basic fish physiology, but also in the fields of pharmacology and environmental toxicology [4–7].

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  1. Aquatic Ecology and Ecotoxicology Group, Department of Zoology, University of Heidelberg, Im Neuenheimer Feld 230, D-69120, Heidelberg, Germany

    Thomas Braunbeck

  2. Department of Chemical Ecotoxicology, UFZ Centre for Environmental Research, Permoserstr. 15, D-04318, Leipzig, Germany

    Helmut Segner

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  1. Thomas Braunbeck
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  2. Helmut Segner
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Michael N. Berry Anthony M. Edwards

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© 2000 Springer Science+Business Media Dordrecht

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Braunbeck, T., Segner, H. (2000). Isolation and cultivation of teleost hepatocytes. In: Berry, M.N., Edwards, A.M. (eds) The Hepatocyte Review. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-3345-8_6

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  • DOI: https://doi.org/10.1007/978-94-017-3345-8_6

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-5402-9

  • Online ISBN: 978-94-017-3345-8

  • eBook Packages: Springer Book Archive

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