Abstract
Various equations for the determination of total chlorophyll and individual amounts of chlorophylls a and b in extracts from plant tissues exist (see Holden 1976) and some of them (e.g. Arnon 1949) have been widely used. Additional modifications to the equations have also been developed so as to permit an estimate of total carotenoids to be made from the spectrum of the same mixture in di-ethyl ether (Ziegler, Egle 1965; Gaudillere 1974).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Arnon DI (1949) Copper enzymes in chloroplasts. Phenol oxidase in Beta vulgaris, Plant Physiol. 24, 1–15.
Gaudillère JP (1974) Amelioration du dosage spectrophotometrique des chlorophylla a et b et des caroténoides totaux dans des extraits foliares, Physiol. Veg. 12, 585–599.
Holden M (1976) Chlorophylls. In Goodwin TO ed. Chemistry and Biochemistry of Plant Pigments, 2nd edn. pp. 462–488, London:Academic Press.
Lichtenthaler HK and Pfister K (1978) Practium der Photosynthese, Heidelberg:Quelle Meyer Verlag. Mackinney G (1941) Absorption of light by chlorophyll solutions, J. biol. Chem. 140, 315–322.
Vishniac W (1957) Methods for study of the Hill reaction. In Colowick SP and Kaplan NO, eds. Methods in Enzymology, Vol. 4, pp. 342–355. New York: Academic Press.
Smith JHC and Benitez A (1956) Chlorophylls:Analysis in plant materials. In Paech K and Tracey MV, eds. Modern methods of plant analysis, vol. 4, pp. 142–196, Berlin:Springer-Verlag.
Wintermans JFGM and de Mots A (1965) Spectrophotometric characteristics of chlorophyll a and b and pheophytins in ethanol, Biochim. Biophys. Acta 109, 448–453.
Ziegler R and Egle K (1965) Zur qualitativen Analyse der Chloroplasten- pigmente, Beitr. Biol. Pflanzen 41, 39–63.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Wellburn, A.R., Lichtenthaler, H. (1984). Formulae and Program to Determine Total Carotenoids and Chlorophylls A and B of Leaf Extracts in Different Solvents. In: Sybesma, C. (eds) Advances in Photosynthesis Research. Advances in Agricultural Biotechnology, vol 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-6368-4_3
Download citation
DOI: https://doi.org/10.1007/978-94-017-6368-4_3
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-247-2943-2
Online ISBN: 978-94-017-6368-4
eBook Packages: Springer Book Archive