Abstract
Gliomas are the most common primary malignant brain tumors and have a very poor prognosis. Urine, which is not controlled by homeostatic mechanisms, can accumulate changes in the body and therefore is an ideal source for biomarker discovery. In this chapter, urine samples of animal models and glioma patients were investigated to identify candidate biomarkers of gliomas using proteomics analysis. The animal model was induced by injection of C6 cells in rat brain. Then rat urine samples were collected on days 2, 6, 10, and 13 after C6 cell inoculation. Urine samples from glioma patients were collected before and after tumor resection. Our results suggested that urinary proteins have potential as early sensitive biomarkers for detection and monitoring gliomas.
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References
An M, Gao Y. Urinary biomarkers of brain diseases. Genomics Proteomics Bioinformatics. 2015;13(6):345–54. https://doi.org/10.1016/j.gpb.2015.08.005.
Bensalma S, Chadeneau C, Legigan T, Renoux B, Gaillard A, de Boisvilliers M, Pinet-Charvet C, Papot S, Muller JM. Evaluation of cytotoxic properties of a cyclopamine glucuronide prodrug in rat glioblastoma cells and tumors. J Mol Neurosci. 2015;55(1):51–61. https://doi.org/10.1007/s12031-014-0395-3.
Cheng YX, Li F, Lu JY, Li M, Du P, Xu GL, Feng H. Growth of G422 glioma implanted in the mouse brain was affected by the immune ability of the host. Chin Med J. 2011;124(13):1994–8.
Doan NB, Nguyen HS, Montoure A, Al-Gizawiy MM, Mueller WM, Kurpad S, Rand SD, Connelly JM, Chitambar CR, Schmainda KM, Mirza SP. Acid ceramidase is a novel drug target for pediatric brain tumors. Oncotarget. 2017;8(15):24753–61. https://doi.org/10.18632/oncotarget.15800.
Gao Y. Urine-an untapped goldmine for biomarker discovery? Sci China Life Sci. 2013;56(12):1145–6. https://doi.org/10.1007/s11427-013-4574-1.
Gao H, Yu B, Yan Y, Shen J, Zhao S, Zhu J, Qin W, Gao Y. Correlation of expression levels of ANXA2, PGAM1, and CALR with glioma grade and prognosis. J Neurosurg. 2013;118(4):846–53. https://doi.org/10.3171/2012.9.JNS112134.
Giatromanolaki A, Sivridis E, Mitrakas A, Kalamida D, Zois CE, Haider S, Piperidou C, Pappa A, Gatter KC, Harris AL, Koukourakis MI. Autophagy and lysosomal related protein expression patterns in human glioblastoma. Cancer Biol Ther. 2014;15(11):1468–78. https://doi.org/10.4161/15384047.2014.955719.
Grobben B, De Deyn PP, Slegers H. Rat C6 glioma as experimental model system for the study of glioblastoma growth and invasion. Cell Tissue Res. 2002;310(3):257–70. https://doi.org/10.1007/s00441-002-0651-7.
Han S, Lv X, Wang Y, Gong H, Zhang C, Tong A, Zhang B, Yao H. Effect and mechanism of peroxisome proliferator-activated receptor-gamma on the drug resistance of the U-87 MG/CDDP human malignant glioma cell line. Mol Med Rep. 2015;12(2):2239–46. https://doi.org/10.3892/mmr.2015.3625.
He Q, Shi X, Zhang L, Yi C, Zhang X, Zhang X. De novo glutamine synthesis: importance for the proliferation of Glioma cells and potentials for its detection with 13N-ammonia. Mol Imaging. 2016;15 https://doi.org/10.1177/1536012116645440.
Hung KS, Howng SL. Prognostic significance of annexin VII expression in glioblastomas multiforme in humans. J Neurosurg. 2003;99(5):886–92. https://doi.org/10.3171/jns.2003.99.5.0886.
Iwadate Y, Matsutani T, Hirono S, Shinozaki N, Saeki N. Transforming growth factor-beta and stem cell markers are highly expressed around necrotic areas in glioblastoma. J Neuro-Oncol. 2016;129(1):101–7. https://doi.org/10.1007/s11060-016-2145-6.
Kore RA, Abraham EC. Inflammatory cytokines, interleukin-1 beta and tumor necrosis factor-alpha, upregulated in glioblastoma multiforme, raise the levels of CRYAB in exosomes secreted by U373 glioma cells. Biochem Biophys Res Commun. 2014;453(3):326–31. https://doi.org/10.1016/j.bbrc.2014.09.068.
Kros JM, Mustafa DM, Dekker LJ, Sillevis Smitt PA, Luider TM, Zheng PP. Circulating glioma biomarkers. Neuro-Oncology. 2015;17(3):343–60. https://doi.org/10.1093/neuonc/nou207.
Kumar DM, Thota B, Shinde SV, Prasanna KV, Hegde AS, Arivazhagan A, Chandramouli BA, Santosh V, Somasundaram K. Proteomic identification of haptoglobin alpha2 as a glioblastoma serum biomarker: implications in cancer cell migration and tumor growth. J Proteome Res. 2010;9(11):5557–67. https://doi.org/10.1021/pr1001737.
Lemke D, Pfenning PN, Sahm F, Klein AC, Kempf T, Warnken U, Schnolzer M, Tudoran R, Weller M, Platten M, Wick W. Costimulatory protein 4IgB7H3 drives the malignant phenotype of glioblastoma by mediating immune escape and invasiveness. Clin Cancer Res. 2012;18(1):105–17. https://doi.org/10.1158/1078-0432.CCR-11-0880.
Liu MF, Jin T, Shen JH, Shen ZY, Zheng ZC, Zhang ZL, Xu LY, Li EM, Xu HX. NGAL and NGALR are frequently overexpressed in human gliomas and are associated with clinical prognosis. J Neuro-Oncol. 2011;104(1):119–27. https://doi.org/10.1007/s11060-010-0486-0.
Liu MF, Hu YY, Jin T, Xu K, Wang SH, Du GZ, Wu BL, Li LY, Xu LY, Li EM, Xu HX. Matrix Metalloproteinase-9/neutrophil Gelatinase-associated Lipocalin complex activity in human Glioma samples predicts tumor presence and clinical prognosis. Dis Markers. 2015;2015:138974. https://doi.org/10.1155/2015/138974.
Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, Burger PC, Jouvet A, Scheithauer BW, Kleihues P. The 2007 WHO classification of tumours of the central nervous system. Acta Neuropathol. 2007;114(2):97–109. https://doi.org/10.1007/s00401-007-0243-4.
Lu J, Ksendzovsky A, Yang C, Mehta GU, Yong RL, Weil RJ, Park DM, Mushlin HM, Fang X, Balgley BM, Lee DH, Lee CS, Lonser RR, Zhuang Z. CNTF receptor subunit alpha as a marker for glioma tumor-initiating cells and tumor grade: laboratory investigation. J Neurosurg. 2012;117(6):1022–31. https://doi.org/10.3171/2012.9.JNS1212.
Martelli C, Iavarone F, D'Angelo L, Arba M, Vincenzoni F, Inserra I, Delfino D, Rossetti DV, Caretto M, Massimi L, Tamburrini G, Di Rocco C, Caldarelli M, Messana I, Castagnola M, Sanna MT, Desiderio C. Integrated proteomic platforms for the comparative characterization of medulloblastoma and pilocytic astrocytoma pediatric brain tumors: a preliminary study. Mol BioSyst. 2015;11(6):1668–83. https://doi.org/10.1039/c5mb00076a.
Matsuura H, Nakazawa S. Prognostic significance of serum alpha 1-acid glycoprotein in patients with glioblastoma multiforme: a preliminary communication. J Neurol Neurosurg Psychiatry. 1985;48(8):835–7.
Monod L, Hamou MF, Ronco P, Verroust P, de Tribolet N. Expression of cALLa/NEP on gliomas: a possible marker of malignancy. Acta Neurochir. 1992;114(1–2):3–7.
Ni Y, Zhang F, An M, Yin W, Gao Y. Early candidate biomarkers found from urine of glioblastoma multiforme rat before changes in MRI. Sci China Life Sci. 2018;61(8):982–7. https://doi.org/10.1007/s11427-017-9201-0.
Nijaguna MB, Schroder C, Patil V, Shwetha SD, Hegde AS, Chandramouli BA, Arivazhagan A, Santosh V, Hoheisel JD, Somasundaram K. Definition of a serum marker panel for glioblastoma discrimination and identification of interleukin 1beta in the microglial secretome as a novel mediator of endothelial cell survival induced by C-reactive protein. J Proteome. 2015;128:251–61. https://doi.org/10.1016/j.jprot.2015.07.026.
Niu H, Wang K, Wang Y. Polymeric immunoglobulin receptor expression is predictive of poor prognosis in glioma patients. Int J Clin Exp Med. 2014;7(8):2185–90.
Ohgaki H, Kleihues P. Population-based studies on incidence, survival rates, and genetic alterations in astrocytic and oligodendroglial gliomas. J Neuropathol Exp Neurol. 2005;64(6):479–89.
Ohtaki S, Wanibuchi M, Kataoka-Sasaki Y, Sasaki M, Oka S, Noshiro S, Akiyama Y, Mikami T, Mikuni N, Kocsis JD, Honmou O. ACTC1 as an invasion and prognosis marker in glioma. J Neurosurg. 2017;126(2):467–75. https://doi.org/10.3171/2016.1.jns152075.
Pan SJ, Zhan SK, Ji WZ, Pan YX, Liu W, Li DY, Huang P, Zhang XX, Cao CY, Zhang J, Bian LG, Sun B, Sun QF. Ubiquitin-protein ligase E3C promotes glioma progression by mediating the ubiquitination and degrading of Annexin A7. Sci Rep. 2015;5:11066. https://doi.org/10.1038/srep11066.
Pei J, Moon KS, Pan S, Lee KH, Ryu HH, Jung TY, Kim IY, Jang WY, Jung CH, Jung S. Proteomic analysis between U87MG and U343MG-A cell lines: searching for candidate proteins for Glioma invasion. Brain Tumor Res Treat. 2014;2(1):22–8. https://doi.org/10.14791/btrt.2014.2.1.22.
Quick Q, Skalli O. Alpha-actinin 1 and alpha-actinin 4: contrasting roles in the survival, motility, and RhoA signaling of astrocytoma cells. Exp Cell Res. 2010;316(7):1137–47. https://doi.org/10.1016/j.yexcr.2010.02.011.
Ramirez-Exposito MJ, Mayas-Torres MD, Carrera-Gonzalez MP, Jimenez-Pulido SB, Illan-Cabeza NA, Sanchez-Sanchez P, Hueso-Urena F, Martinez-Martos JM, Moreno-Carretero MN. Silver(I)/6-hydroxyiminolumazine compounds differently modify renin-angiotensin system-regulating aminopeptidases a and N in human neuroblastoma and glioma cells. J Inorg Biochem. 2014;138:56–63. https://doi.org/10.1016/j.jinorgbio.2014.04.019.
Setti M, Osti D, Richichi C, Ortensi B, Del Bene M, Fornasari L, Beznoussenko G, Mironov A, Rappa G, Cuomo A, Faretta M, Bonaldi T, Lorico A, Pelicci G. Extracellular vesicle-mediated transfer of CLIC1 protein is a novel mechanism for the regulation of glioblastoma growth. Oncotarget. 2015;6(31):31413–27. https://doi.org/10.18632/oncotarget.5105.
Shao C, Li M, Li X, Wei L, Zhu L, Yang F, Jia L, Mu Y, Wang J, Guo Z, Zhang D, Yin J, Wang Z, Sun W, Zhang Z, Gao Y. A tool for biomarker discovery in the urinary proteome: a manually curated human and animal urine protein biomarker database. Mol Cell Proteomics. 2011;10(11):M111 010975. https://doi.org/10.1074/mcp.M111.010975.
Shimizu M, Tanaka M, Atomi Y. Small heat shock protein alphaB-Crystallin controls shape and adhesion of Glioma and myoblast cells in the absence of stress. PLoS One. 2016;11(12):e0168136. https://doi.org/10.1371/journal.pone.0168136.
Smith ER, Zurakowski D, Saad A, Scott RM, Moses MA. Urinary biomarkers predict brain tumor presence and response to therapy. Clin Cancer Res. 2008;14(8):2378–86. https://doi.org/10.1158/1078-0432.CCR-07-1253.
Stavrinou P, Mavrogiorgou MC, Polyzoidis K, Kreft-Kerekes V, Timmer M, Marselos M, Pappas P. Expression profile of genes related to drug metabolism in human brain Tumors. PLoS One. 2015;10(11):e0143285. https://doi.org/10.1371/journal.pone.0143285.
Tynninen O, Carpen O, Jaaskelainen J, Paavonen T, Paetau A. Ezrin expression in tissue microarray of primary and recurrent gliomas. Neuropathol Appl Neurobiol. 2004;30(5):472–7. https://doi.org/10.1111/j.1365-2990.2004.00562.x.
Wang JY, Bettegowda C. Genetics and immunotherapy: using the genetic landscape of gliomas to inform management strategies. J Neuro-Oncol. 2015;123(3):373–83. https://doi.org/10.1007/s11060-015-1730-4.
Wu J, Gao Y. Physiological conditions can be reflected in human urine proteome and metabolome. Expert Rev Proteomics. 2015;12(6):623–36. https://doi.org/10.1586/14789450.2015.1094380.
Wu J, Zhang J, Zhao Y, Gao Y. Candidate urine biomarker discovery from only five pairs of samples before and after tumor resection in glioma patients. bioRxiv; 2018. https://doi.org/10.1101/240861.
Xu G, Li W, Zhang P, Ding Z, Zhao H, Zhang J, Wang M. Silencing of carcinoembryonic antigen-related cell adhesion molecule 1 inhibits proliferation and induces apoptosis in human glioma SHG44 cells. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015;31(1):23–6.. 31
Yan H, Yang K, Xiao H, Zou YJ, Zhang WB, Liu HY. Over-expression of cofilin-1 and phosphoglycerate kinase 1 in astrocytomas involved in pathogenesis of radioresistance. CNS Neurosci Ther. 2012;18(9):729–36. https://doi.org/10.1111/j.1755-5949.2012.00353.x.
Zheng LT, Lee S, Yin GN, Mori K, Suk K. Down-regulation of lipocalin 2 contributes to chemoresistance in glioblastoma cells. J Neurochem. 2009;111(5):1238–51. https://doi.org/10.1111/j.1471-4159.2009.06410.x.
Zhou Z, Luther N, Ibrahim GM, Hawkins C, Vibhakar R, Handler MH, Souweidane MM. B7-H3, a potential therapeutic target, is expressed in diffuse intrinsic pontine glioma. J Neuro-Oncol. 2013;111(3):257–64. https://doi.org/10.1007/s11060-012-1021-2.
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Wu, J., Ni, Y. (2019). Candidate Urinary Biomarker Discovery in Gliomas. In: Gao, Y. (eds) Urine. Springer, Singapore. https://doi.org/10.1007/978-981-13-9109-5_10
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DOI: https://doi.org/10.1007/978-981-13-9109-5_10
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