Abstract
Nanoparticles range from 1 to 100 nm in size, and though the size is in nanometers, its application is in broad areas such as biomedical, industry, food, and cosmetics. With increasing utilization, the toxicity of the nanoparticle has been a great concern to evaluate their potential. To use the nanoparticle effectively, it is necessary to know the toxicity of NP and different evaluation methods and characteristics. “Dosimetry: Too complicated to consider, too important to ignore” as stated by Dr. Philip Demokritou in the seventh International Nanotoxicology Congress; Dosimetry is one of the important factors besides the surface area and high reactivity to determine the toxicity nature of the NP. Every NP may not show the same toxicity; it varies with the material it is made up of, site of its action, and exposure routes. This chapter addresses the current knowledge of evaluation of nanosized particles toxicity using in vitro derived cell lines from different literature, as a primary step for screening their toxicological effects, which contributes to the further development and advancement of nanotechnology on a safe, unbiased level. The in vitro derived cell lines however does not ensure the same cell habitat as in the tissue, as nanoparticles interact with proteins and physiological barriers, immune response in the tissue has a more complex environment. Hence, these in vitro evaluation methods give us a base for further considering the nanoparticle potential and its toxicity.
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Pola, S., Konatala, A. (2020). Evaluation of Toxicity of Nanoparticles Using Cell Lines. In: Siddhardha, B., Dyavaiah, M., Kasinathan, K. (eds) Model Organisms to Study Biological Activities and Toxicity of Nanoparticles. Springer, Singapore. https://doi.org/10.1007/978-981-15-1702-0_15
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