Abstract
Statins, a class of drugs commercially available to treat atherosclerosis and various cardiovascular diseases that come under the same umbrella but these drugs have various side-effects, like low bioavailability and less solubility in human body, associated with them. Earlier, we synthesized herbal nano-statins from mushroom as an alternative solution to combat this problem. The present work demonstrates synthesis and characterization of nanoparticles of statins in two different solvents namely, acetonitrile (ACN) and dimethyl sulfoxide (DMSO). The cytotoxicity of the synthesized nanoparticles of statins was evaluated using HepG2 cell lines, MTT assay was performed and the results showed that nanoparticles of statins synthesized in DMSO showed significant reduction in cell viability of cancerous cells even at lower doses (50–60 µL) without any significant effect on normal cells. Thus, nanoparticles of statins synthesized in DMSO were proven to be effective to treat cancerous cells, whereas nanoparticles of statins synthesized in acetonitrile were comparatively less effective with only 30–35% cell death even at higher doses (100 µL). Also, it is found to be toxic for normal cells, hampering its overall potential to act as anti-atherosclerotic. This study provides us a platform to further evaluate nano-statins synthesized in DMSO for their anti-atherosclerotic and anti-inflammatory potential by evaluating their efficacy in endothelial cells.
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References
A. Boutayeb, The double burden of communicable and non-communicable diseases in developing countries. Trans. R. Soc. Trop. Med. Hyg. 100(3), 191–199 (2006)
P. Kotyla, The role of 3-hydroxy-3-methylglutaryl coenzyme a reductase inhibitors (statins) in modern rheumatology. Therapeutic Adv. Musculoskeletal Disease 2(5), 257–269 (2010)
C.M. Minder, M.J. Blaha, A. Horne, E.D. Michos, S. Kaul, R.S. Blumenthal, Evidence-based use of statins for primary prevention of cardiovascular disease. Am. J. Med. 125(5), 440–446 (2012)
N.C. Ward, G.F. Watts, R.H. Eckel, Statin toxicity: mechanistic insights and clinical implications. Circ. Res. 124(2), 328–350 (2019)
C.B. Newman, D. Preiss, J.A. Tobert, T.A. Jacobson, R.L. Page, L.B. Goldstein, C. Chin, L.R. Tannock, M. Miller, G. Raghuveer, Statin safety and associated adverse events: a scientific statement from the American Heart Association. Arterioscler. Thromb. Vasc. Biol. 39(2), e38–e81 (2019)
B. Romana, M. Batger, C. Prestidge, G. Colombo, F. Sonvico, Expanding the therapeutic potential of statins by means of nanotechnology enabled drug delivery systems. Curr. Top. Medicinal Chem. 14(9), 1182–1193 (2014)
A. Tachjian, V. Maria, A. Jahangir, Use of herbal products and potential interactions in patients with cardiovascular diseases. J. Am. Coll. Cardiol. 55(6), 515–525 (2010)
A. Fugh-Berman, Herbs and dietary supplements in the prevention and treatment of cardiovascular disease. Preventive Cardiol. 3(1), 24–32 (2000)
K. Chakraborty, A. Shivakumar, S. Ramachandran, Nano-technology in herbal medicines: a review. Int. J. Herbal Med. 4(3), 21–27 (2016)
A. Mehra, R. Narang, V. Jain, S. Nagpal, Preparation and characterization of nano statins using oyster mushroom (Pleurotus sajor-caju): a new strategy to reduce toxicity and enhance efficacy for the treatment of cardiovascular disease. Euro. J. Integrative Med. 33, 101014 (2020)
D.N. De Assis, V.C.F. Mosqueira, J.M.C. Vilela, M.S. Andrade, V.N. Cardoso, Release profiles and morphological characterization by atomic force microscopy and photon correlation spectroscopy of 99mTechnetium-fluconazole nanocapsules. Int. J. Pharm. 349(1–2), 152–160 (2008)
R. Bhargava, I.W. Levin, Fourier transform infrared imaging: theory and practice. Anal. Chem. 73(21), 5157–5167 (2001)
M. Piecha, M. Sarakha, P. Trebše, D. Kočar, Stability studies of cholesterol lowering statin drugs in aqueous samples using HPLC and LC–MS. Environ. Chem. Lett. 8(2), 185–191 (2010)
N.M. Badawi, M.H. Teaima, K.M. El-Say, D.A. Attia, M.A. El-Nabarawi, M.M. Elmazar, Pomegranate extract-loaded solid lipid nanoparticles: design, optimization, and in vitro cytotoxicity study. Int. J. Nanomed. 13, 1313 (2018)
Acknowledgements
This research was supported by Amity University, Noida, Uttar Pradesh. We would also like to show our gratitude to Dr. Ashok K. Chauhan, Founder President, Amity University for their continuous encouragement and support.
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Mehra, A., Chauhan, S., Narang, R., Jain, V.K., Nagpal, S. (2020). Nanoparticles of Statins Synthesized from Mushroom Using Two Different Solvents and Evaluating Their Cytotoxic Potential Using HepG2 Cell Lines. In: Jain, V.K., Rattan, S., Verma, A. (eds) Recent Trends in Materials and Devices. Springer Proceedings in Physics, vol 256. Springer, Singapore. https://doi.org/10.1007/978-981-15-8625-5_14
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DOI: https://doi.org/10.1007/978-981-15-8625-5_14
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