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Genetic Engineering of Cell Cultures for Enhanced Production of Nutraceuticals

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Nutraceuticals Production from Plant Cell Factory

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Abstract

Driven by various health concerns, there is growing demand for plant-derived ingredients, used as pharmaceutical alternatives that claim certain physiological benefits for the human body. As such, nutraceuticals are defined as bioactive natural substances in the tissue of many plant species and mainly used to prevent human diseases, due to their anti-inflammatory and antioxidant properties. The emerging trend for manufacturing nutraceuticals in the form of dietary supplements or functional foods in a natural and sustainable manner has opened new avenues of plant cell culture technology over the past years. The advantages of this technology opposed to the wild harvesting or field cultivation are the controlled production according to the demand, preservation of the global biodiversity, as well as avoidance of associated ecological restrictions. Moreover, genetic engineering is a tool that provides a great opportunity for metabolic manipulation through overexpression, silencing, or disruption of one or more genes of the biosynthetic pathway of interest. In this chapter, we attempt to provide a framework of the cellular and molecular tools and their application regarding the enhanced production of nutraceuticals from plant cell cultures with special focus on flavonoids as ubiquitous metabolites in plant foods, including vegetables, fruits, nuts, and beverages possessing many beneficial effects over the human body.

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Acknowledgments

This research received funding from the European Union’s Horizon 2020 research and innovation programme project PlantaSYST (SGA no. 739582 FPA no. 664620), and the BG05M2OP001-1.003-001-C01 project, financed by the European Regional Development Fund through the “Science and Education for Smart Growth” Operational Programme.

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Authors and Affiliations

  1. Laboratory of Metabolomics, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Plovdiv, Bulgaria

    Andrey Marchev, Kristiana Amirova & Milen Georgiev

  2. Center of Plant Systems Biology and Biotechnology, Plovdiv, Bulgaria

    Andrey Marchev, Kristiana Amirova & Milen Georgiev

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  1. Andrey Marchev
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  2. Kristiana Amirova
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  3. Milen Georgiev
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Correspondence to Milen Georgiev .

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Editors and Affiliations

  1. Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China

    Tarun Belwal

  2. Institute of Microbiology, Bulgarian Academy of Sciences, Plovdiv, Bulgaria

    Milen I. Georgiev

  3. Agricultural Biotechnology, King Faisal University, Al-Hassa, Saudi Arabia

    Jameel M Al-Khayri

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Marchev, A., Amirova, K., Georgiev, M. (2022). Genetic Engineering of Cell Cultures for Enhanced Production of Nutraceuticals. In: Belwal, T., Georgiev, M.I., Al-Khayri, J.M. (eds) Nutraceuticals Production from Plant Cell Factory. Springer, Singapore. https://doi.org/10.1007/978-981-16-8858-4_16

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