Abstract
The computer vision domain has seen increasing attention in the design of automated tools for cellular biology researchers. In addition to quantitative analysis on whole populations of cells, identification of the cell division events is another important topic. In this research, a novel fully automated image-based cell-division-detection approach is proposed. Differing from most of the existing approaches that exploit training-based or image-based segmentation methods, the main idea of the proposed approach is detecting cell divisions using a motion based occlusion analysis process. Testing has been performed on different types of cellular datasets, including fluorescence images and phase-contrast data, and it has confirmed the effectiveness of the proposed method.
Thanks the National Biophotonics and Imaging Platform Ireland (NBIPI).
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Notes
- 1.
The rate of the correct automatically detected division number out of the total automatically detected number.
- 2.
The rate of the correct automatically detected division number out of the total manually annotated number.
References
Huh S, Chen M (2011) Detection of mitosis within a stem cell population of high cell confluence in phase-contrast microscopy images. In: IEEE computer vision and pattern recognition (CVPR’11), pp 1033–1040
Padfield DR, Rittscher J, Thomas N, Roysam B (2009) Spatio-temporal cell cycle phase analysis using level sets and fast marching methods. Med Image Anal 13(1):143–155
Bunyak F, Palaniappan K, Nath SK, Baskin TI, Dong G (2006) Quantitative cell motility for in vitro wound healing using level set-based active contour tracking. In: Proceedings of the 3rd IEEE international symposium biomedical imaging (ISBI), 1040–1043 April 2006
Li K, Miller ED, Chen M, Kanade T, Weiss LE, Campbell PG (2008) Cell population tracking and lineage construction with spatiotemporal context. Med Image Anal 12(5):546–566
Li F, Zhou X, Ma J, Wong ST (2010) Multiple nuclei tracking using integer programming for quantitative cancer cell cycle analysis. IEEE Trans Med Imaging 29(1):96–105
Kanade T, Yin Z, Bise R, Huh S, Eom SE, Sandbothe M, Chen M (2011) Cell image analysis: algorithms, system and applications. In: IEEE workshop on applications of computer vision (WACV)
Quelhas P, Mendona A, Campilho A (2010) Optical flow based Arabidopsis thaliana root meristem cell division detection. Lect Notes Comput Sci 6112:217–226
Horn BKP, Schunk BG (1981) Determining optical flow. Artif Intell 17:185–203
Brox T, Bruhn A, Papenberg N, Weickert J (2004) High accuracy optical flow estimation based on a theory for warping. In: Computer vision—proceedings of 8th European conference on computer vision, 2004
Lucas BD, Kanade T (1981) An iterative image registration technique with an application to stereo vision. In: IJCAI, vol 81, pp 674–679
Thirion JP (1998) Image matching as a diffusion process: an analogy with Maxwell’s demons. Med Image Anal 2(3):243–260
Alvarez L, Deriche R, Papadopoulo T, Sanchez J (2002) Symmetrical dense optical flow estimation with occlusion detection. In: European conference on computer vision. Springer, pp 721–735
Cheng Y (1995) Mean shift, mode seeking, and clustering. IEEE Trans Pattern Anal Mach Intell 17:790–799
Acknowledgments
This research was supported by the National Biophotonics Imaging Platform (NBIP) Ireland funded under the Higher Education Authority PRTLI Cycle 4, co-funded by the Irish Government and the European Union—Investing in your future.
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Yu, S., Molloy, D. (2014). Optic Flow Based Occlusion Analysis for Cell Division Detection. In: Mat Sakim, H., Mustaffa, M. (eds) The 8th International Conference on Robotic, Vision, Signal Processing & Power Applications. Lecture Notes in Electrical Engineering, vol 291. Springer, Singapore. https://doi.org/10.1007/978-981-4585-42-2_55
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DOI: https://doi.org/10.1007/978-981-4585-42-2_55
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