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Genetics and genetic engineering of the industrial yeast Yarrowia lipolytica

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Applied Molecular Genetics

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 43))

Abstract

Y. lipolytica can be grown on hydrocarbons, and has been used to make single cell proteins and citric acid. The existence of sexuality allowed the performance of inbreeding programs leading to strains that can be utilized for classical genetic analysis. Physiological and genetic studies have been carried out on hydrocarbon utilization, lysine biosynthesis and catabolism, and protein secretion.

Due to the lack of plasmids, transformation of Y. lipolytica was first achieved by chromosomal integration, using either homologous or heterologous genes as markers. In a second step, the isolation of ars sequences allowed the building up autoreplicating vectors. A number of heterologous genes have been expressed in Y. lipolytica, such as those coding for calf prochymosin, S. cerevisiae invertase and porcine alpha-1-interferon. These proteins are secreted.

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  1. Chaire de Génétique Moléculaire et Cellulaire, Institut National Agronomique, 16 Rue Claude Bernard, 75231, Paris Cédex 05, France

    H. Heslot

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© 1990 Springer-Verlag

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Heslot, H. (1990). Genetics and genetic engineering of the industrial yeast Yarrowia lipolytica . In: Applied Molecular Genetics. Advances in Biochemical Engineering/Biotechnology, vol 43. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0009079

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  • DOI: https://doi.org/10.1007/BFb0009079

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  • Print ISBN: 978-3-540-52794-7

  • Online ISBN: 978-3-540-47151-6

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