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Preparation of Primary Cultures of Mouse Epidermal Keratinocytes and the Measurement of Phospholipase D Activity

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Epidermal Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1195))

Abstract

In this chapter information is provided about the outer layer of the skin, the epidermis, and the predominant cells comprising this epithelium, the keratinocytes. The evidence supporting a possible role for the lipid-metabolizing enzyme phospholipase D in regulating keratinocyte differentiation is also discussed. A detailed protocol for the preparation of primary cultures of epidermal keratinocytes from neonatal mice is described, to allow other investigators to obtain data concerning these important cells involved in forming and maintaining the mechanical and water permeability of the skin. Finally, a complete protocol for monitoring phospholipase D activity in intact cells is supplied in the hope that additional research will result in a better understanding of the role of phospholipase D in controlling keratinocyte proliferation and differentiation.

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Acknowledgements

W.B.B. is supported by VA Merit Award #CX000590 and a VA Research Career Scientist Award. The contents of this article do not represent the views of the Department of Veterans Affairs or the United States Government.

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Authors and Affiliations

  1. Department of Physiology, Georgia Regents University, 1120 15th Street, Augusta, GA, 30912, USA

    Lakiea J. Bailey, Vivek Choudhary, Purnima Merai & Wendy B. Bollag

  2. Charlie Norwood VA Medical Center, One Freedom Way, Augusta, GA, 30904, USA

    Vivek Choudhary, Purnima Merai & Wendy B. Bollag

Authors
  1. Lakiea J. Bailey
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  2. Vivek Choudhary
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  3. Purnima Merai
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  4. Wendy B. Bollag
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Corresponding author

Correspondence to Wendy B. Bollag .

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Editors and Affiliations

  1. Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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Bailey, L.J., Choudhary, V., Merai, P., Bollag, W.B. (2014). Preparation of Primary Cultures of Mouse Epidermal Keratinocytes and the Measurement of Phospholipase D Activity. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology, vol 1195. Springer, New York, NY. https://doi.org/10.1007/7651_2014_80

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  • DOI: https://doi.org/10.1007/7651_2014_80

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-1223-0

  • Online ISBN: 978-1-4939-1224-7

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