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Rapid Amplification of cDNA Ends for RNA Transcript Sequencing in Staphylococcus

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The Genetic Manipulation of Staphylococci

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1373))

Abstract

Rapid amplification of cDNA ends (RACE) is a technique that was developed to swiftly and efficiently amplify full-length RNA molecules in which the terminal ends have not been characterized. Current usage of this procedure has been more focused on sequencing and characterizing RNA 5′ and 3′ untranslated regions. Herein is described an adapted RACE protocol to amplify bacterial RNA transcripts.

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Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, School of Medicine, University of Nevada Reno, 1664 N. Virginia Street, Reno, NV, 89557, USA

    Eric Miller

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  1. Eric Miller
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Correspondence to Eric Miller .

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Editors and Affiliations

  1. University of Kansas Medical Center, Kansas City, Kansas, USA

    Jeffrey L. Bose

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© 2015 Springer Science+Business Media New York

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Miller, E. (2015). Rapid Amplification of cDNA Ends for RNA Transcript Sequencing in Staphylococcus. In: Bose, J. (eds) The Genetic Manipulation of Staphylococci. Methods in Molecular Biology, vol 1373. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_282

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  • DOI: https://doi.org/10.1007/7651_2015_282

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3157-6

  • Online ISBN: 978-1-4939-3158-3

  • eBook Packages: Springer Protocols

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