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Methylation Analysis in Distinct Immune Cell Subsets in Type 1 Diabetes

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Type-1 Diabetes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1433))

Abstract

Epigenetics provides a mechanism in which the environment can interact with the genotype to produce a variety of phenotypes. These epigenetic modifications have been associated with altered gene expression and silencing of repetitive elements, and these modifications can be inherited mitotically. DNA methylation is the best characterized epigenetic mark and earlier studies have examined DNA methylation profiles in peripheral blood mononuclear cells in disease. However, any disease-related signatures identified would just display differences in the relative abundance of individual cell types as each cell subset generates a unique methylation profile. Therefore is it important to identify cell- or tissue-specific changes in DNA methylation, particularly in autoimmune diseases such as type 1 diabetes.

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Acknowledgement

This work was supported by JDRF and BLUEPRINT EU-FP7.

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Authors and Affiliations

  1. Centre for Diabetes and Metabolic Medicine, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, 4 Newark Street, London, E1 2AT, UK

    Mary N. Dang, Claire M. Bradford, Paolo Pozzilli & R. David Leslie

  2. Department of Endocrinology and Diabetes, University Campus Bio-Medico of Rome, Via Alvaro del Portillo, 21, 00128, Rome, Italy

    Paolo Pozzilli

Authors
  1. Mary N. Dang
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  2. Claire M. Bradford
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  3. Paolo Pozzilli
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  4. R. David Leslie
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Corresponding author

Correspondence to Mary N. Dang .

Editor information

Editors and Affiliations

  1. Diabetes and Metabolism Unit, School of Clinical Sciences, Southmead Hospital, University of Bristol, Bristol, United Kingdom

    Kathleen M. Gillespie

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© 2015 Springer Science+Business Media New York

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Dang, M.N., Bradford, C.M., Pozzilli, P., Leslie, R.D. (2015). Methylation Analysis in Distinct Immune Cell Subsets in Type 1 Diabetes. In: Gillespie, K. (eds) Type-1 Diabetes. Methods in Molecular Biology, vol 1433. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_286

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  • DOI: https://doi.org/10.1007/7651_2015_286

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3641-0

  • Online ISBN: 978-1-4939-3643-4

  • eBook Packages: Springer Protocols

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